Restriction map for
pFN21AE4396
(Linear) (Six-base) MAPSORT of: KIEE4396.seq Check: 7273 from: 1 to: 1251
KIEE4396.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 373 1251
Size: 373 878
AatII G_ACGT'C
Cuts at: 0 86 1251
Size: 86 1165
Acc65I G'GTAC_C
Cuts at: 0 764 1251
Size: 764 487
AhdI GACnn_n'nnGTC
Cuts at: 0 848 1251
Size: 848 403
AleI CACnn'nnGTG
Cuts at: 0 106 1251
Size: 106 1145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 962 994 1204 1236 1251
Size: 962 32 210 32 15
Fragments arranged by size:
962 210 32 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 601 614 699 891 1251
Size: 601 13 85 192 360
Fragments arranged by size:
601 360 192 85 13
ApaI G_GGCC'C
Cuts at: 0 250 1251
Size: 250 1001
ApoI r'AATT_y
Cuts at: 0 277 1251
Size: 277 974
AvaI C'yCGr_G
Cuts at: 0 5 1146 1251
Size: 5 1141 105
Fragments arranged by size:
1141 105 5
AvrII C'CTAG_G
Cuts at: 0 951 1251
Size: 951 300
BamHI G'GATC_C
Cuts at: 0 1227 1251
Size: 1227 24
BanI G'GyrC_C
Cuts at: 0 96 108 156 573 624 764 880 1251
Size: 96 12 48 417 51 140 116 371
Fragments arranged by size:
417 371 140 116 96 51 48 12
BanII G_rGCy'C
Cuts at: 0 250 821 960 1251
Size: 250 571 139 291
Fragments arranged by size:
571 291 250 139
BbeI G_GCGC'C
Cuts at: 0 100 884 1251
Size: 100 784 367
Fragments arranged by size:
784 367 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 1251
Size: 222 1029
BbvCI CC'TCA_GC
Cuts at: 0 322 446 692 1170 1251
Size: 322 124 246 478 81
Fragments arranged by size:
478 322 246 124 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 555 589 1251
Size: 555 34 662
Fragments arranged by size:
662 555 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 747 1251
Size: 747 504
Bme1580I G_kGCm'C
Cuts at: 0 113 161 250 485 629 1246 1251
Size: 113 48 89 235 144 617 5
Fragments arranged by size:
617 235 144 113 89 48 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 995 1251
Size: 304 691 256
Fragments arranged by size:
691 304 256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1233 1251
Size: 1233 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1251
Size: 491 760
Bpu10I CC'TnA_GC
Cuts at: 0 322 446 692 1170 1251
Size: 322 124 246 478 81
Fragments arranged by size:
478 322 246 124 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1251
Size: 615 636
BsaHI Gr'CG_yC
Cuts at: 0 83 97 881 1251
Size: 83 14 784 370
Fragments arranged by size:
784 370 83 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 464 494 1251
Size: 464 30 757
Fragments arranged by size:
757 464 30
BseYI C'CCAG_C
Cuts at: 0 250 884 1074 1251
Size: 250 634 190 177
Fragments arranged by size:
634 250 190 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1251
Size: 112 1139
BsiHKAI G_wGCw'C
Cuts at: 0 674 841 960 1010 1251
Size: 674 167 119 50 241
Fragments arranged by size:
674 241 167 119 50
BsmI GAATG_Cn'
Cuts at: 0 318 1251
Size: 318 933
BsmBI CGTCTCn'nnnn_
Cuts at: 0 504 1251
Size: 504 747
Bsp1286I G_dGCh'C
Cuts at: 0 113 161 250 485 629 674 821 841
Size: 113 48 89 235 144 45 147 20
Cuts at: 841 960 1010 1246 1251
Size: 119 50 236 5
Fragments arranged by size:
236 235 147 144 119 113 89 50
48 45 20 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 373 667 802 1251
Size: 373 294 135 449
Fragments arranged by size:
449 373 294 135
BssSI C'ACGA_G
Cuts at: 0 673 1251
Size: 673 578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 469 1251
Size: 273 196 782
Fragments arranged by size:
782 273 196
BstYI r'GATC_y
Cuts at: 0 1227 1251
Size: 1227 24
BtgI C'CryG_G
Cuts at: 0 11 485 845 1251
Size: 11 474 360 406
Fragments arranged by size:
474 406 360 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 812 1251
Size: 812 439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 620 655 1251
Size: 620 35 596
Fragments arranged by size:
620 596 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 180 1251
Size: 180 1071
EaeI y'GGCC_r
Cuts at: 0 14 25 61 224 771 1251
Size: 14 11 36 163 547 480
Fragments arranged by size:
547 480 163 36 14 11
EarI CTCTTCn'nnn_
Cuts at: 0 1224 1251
Size: 1224 27
EcoICRI GAG'CTC
Cuts at: 0 958 1251
Size: 958 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1251
Size: 491 760
EcoNI CCTnn'n_nnAGG
Cuts at: 0 321 1251
Size: 321 930
EcoO109I rG'GnC_Cy
Cuts at: 0 246 247 850 994 1119 1251
Size: 246 1 603 144 125 132
Fragments arranged by size:
603 246 144 132 125 1
EcoRI G'AATT_C
Cuts at: 0 277 1251
Size: 277 974
EcoRV GAT'ATC
Cuts at: 0 408 1251
Size: 408 843
HaeII r_GCGC'y
Cuts at: 0 46 100 884 1169 1251
Size: 46 54 784 285 82
Fragments arranged by size:
784 285 82 54 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 834 866 1251
Size: 834 32 385
Fragments arranged by size:
834 385 32
KasI G'GCGC_C
Cuts at: 0 96 880 1251
Size: 96 784 371
Fragments arranged by size:
784 371 96
KpnI G_GTAC'C
Cuts at: 0 768 1251
Size: 768 483
MscI TGG'CCA
Cuts at: 0 63 773 1251
Size: 63 710 478
Fragments arranged by size:
710 478 63
MslI CAynn'nnrTG
Cuts at: 0 106 169 844 1251
Size: 106 63 675 407
Fragments arranged by size:
675 407 106 63
MspA1I CmG'CkG
Cuts at: 0 30 189 445 494 696 1198 1251
Size: 30 159 256 49 202 502 53
Fragments arranged by size:
502 256 202 159 53 49 30
NarI GG'CG_CC
Cuts at: 0 97 881 1251
Size: 97 784 370
Fragments arranged by size:
784 370 97
NcoI C'CATG_G
Cuts at: 0 11 485 845 1251
Size: 11 474 360 406
Fragments arranged by size:
474 406 360 11
NspI r_CATG'y
Cuts at: 0 181 1020 1162 1186 1251
Size: 181 839 142 24 65
Fragments arranged by size:
839 181 142 65 24
PasI CC'CwG_GG
Cuts at: 0 876 1251
Size: 876 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 614 1251
Size: 614 637
PpuMI rG'GwC_Cy
Cuts at: 0 850 994 1119 1251
Size: 850 144 125 132
Fragments arranged by size:
850 144 132 125
PspOMI G'GGCC_C
Cuts at: 0 246 1251
Size: 246 1005
PspXI vC'TCGA_Gb
Cuts at: 0 1146 1251
Size: 1146 105
PvuII CAG'CTG
Cuts at: 0 189 445 696 1198 1251
Size: 189 256 251 502 53
Fragments arranged by size:
502 256 251 189 53
SacI G_AGCT'C
Cuts at: 0 960 1251
Size: 960 291
SanDI GG'GwC_CC
Cuts at: 0 850 1251
Size: 850 401
SapI GCTCTTCn'nnn_
Cuts at: 0 1224 1251
Size: 1224 27
SfcI C'TryA_G
Cuts at: 0 151 1251
Size: 151 1100
SfoI GGC'GCC
Cuts at: 0 98 882 1251
Size: 98 784 369
Fragments arranged by size:
784 369 98
SmlI C'TyrA_G
Cuts at: 0 630 1146 1251
Size: 630 516 105
Fragments arranged by size:
630 516 105
SphI G_CATG'C
Cuts at: 0 1162 1251
Size: 1162 89
StyI C'CwwG_G
Cuts at: 0 11 485 768 845 951 1251
Size: 11 474 283 77 106 300
Fragments arranged by size:
474 300 283 106 77 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 1251
Size: 118 1133
TatI w'GTAC_w
Cuts at: 0 719 1150 1251
Size: 719 431 101
Fragments arranged by size:
719 431 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 1251
Size: 464 32 755
Fragments arranged by size:
755 464 32
Tth111I GACn'n_nGTC
Cuts at: 0 85 1251
Size: 85 1166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1038 1251
Size: 1038 213
XhoI C'TCGA_G
Cuts at: 0 1146 1251
Size: 1146 105
ZraI GAC'GTC
Cuts at: 0 84 1251
Size: 84 1167
Enzymes that do cut:
AarI AatII Acc65I AhdI AleI AloI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BssSI BstXI BstYI BtgI BtgZI CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I KasI KpnI MscI MslI MspA1I
NarI NcoI NspI PasI PflMI PpuMI PspOMI PspXI
PvuII SacI SanDI SapI SfcI SfoI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AlfI
ApaLI AscI AseI AsiSI BaeI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaWI
BseRI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI
ClaI DraI DraIII EagI EciI FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XmaI XmnI