Restriction map for
pFN21AE4391
(Linear) (Six-base) MAPSORT of: KIEE4391.seq Check: 5082 from: 1 to: 1242
KIEE4391.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 852 1242
Size: 852 390
AccI GT'mk_AC
Cuts at: 0 911 1242
Size: 911 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 859 976 1093 1242
Size: 294 565 117 117 149
Fragments arranged by size:
565 294 149 117 117
AflIII A'CryG_T
Cuts at: 0 914 1242
Size: 914 328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 1242
Size: 619 623
AlwNI CAG_nnn'CTG
Cuts at: 0 429 1125 1242
Size: 429 696 117
Fragments arranged by size:
696 429 117
ApaLI G'TGCA_C
Cuts at: 0 1057 1242
Size: 1057 185
ApoI r'AATT_y
Cuts at: 0 504 1242
Size: 504 738
BanI G'GyrC_C
Cuts at: 0 265 711 943 1108 1242
Size: 265 446 232 165 134
Fragments arranged by size:
446 265 232 165 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 49 382 967 1242
Size: 49 333 585 275
Fragments arranged by size:
585 333 275 49
BglII A'GATC_T
Cuts at: 0 330 1242
Size: 330 912
BlpI GC'TnA_GC
Cuts at: 0 1067 1242
Size: 1067 175
Bme1580I G_kGCm'C
Cuts at: 0 270 716 946 1061 1242
Size: 270 446 230 115 181
Fragments arranged by size:
446 270 230 181 115
BmgBI CAC'GTC
Cuts at: 0 915 1242
Size: 915 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 861 1242
Size: 709 152 381
Fragments arranged by size:
709 381 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 249 1242
Size: 163 86 993
Fragments arranged by size:
993 163 86
BsaWI w'CCGG_w
Cuts at: 0 1111 1242
Size: 1111 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 914 944 1242
Size: 859 30 25 30 298
Fragments arranged by size:
859 298 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 1242
Size: 31 1211
BsiHKAI G_wGCw'C
Cuts at: 0 1061 1119 1208 1242
Size: 1061 58 89 34
Fragments arranged by size:
1061 89 58 34
Bsp1286I G_dGCh'C
Cuts at: 0 270 716 946 1061 1119 1208 1242
Size: 270 446 230 115 58 89 34
Fragments arranged by size:
446 270 230 115 89 58 34
BspHI T'CATG_A
Cuts at: 0 336 1242
Size: 336 906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 852 1242
Size: 852 390
BssSI C'ACGA_G
Cuts at: 0 361 1242
Size: 361 881
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 1242
Size: 30 1212
BstYI r'GATC_y
Cuts at: 0 330 1242
Size: 330 912
BtgI C'CryG_G
Cuts at: 0 555 1130 1242
Size: 555 575 112
Fragments arranged by size:
575 555 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1015 1242
Size: 1015 227
EaeI y'GGCC_r
Cuts at: 0 20 1242
Size: 20 1222
EarI CTCTTCn'nnn_
Cuts at: 0 1213 1242
Size: 1213 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 709 859 861 976 1093 1242
Size: 294 415 150 2 115 117 149
Fragments arranged by size:
415 294 150 149 117 115 2
EcoO109I rG'GnC_Cy
Cuts at: 0 384 1242
Size: 384 858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 87 207 1242
Size: 87 120 1035
Fragments arranged by size:
1035 120 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 859 891 1011 1043 1242
Size: 748 32 79 32 120 32 199
Fragments arranged by size:
748 199 120 79 32 32 32
HincII GTy'rAC
Cuts at: 0 1227 1242
Size: 1227 15
HindIII A'AGCT_T
Cuts at: 0 92 1242
Size: 92 1150
HpaI GTT'AAC
Cuts at: 0 1227 1242
Size: 1227 15
MscI TGG'CCA
Cuts at: 0 22 1242
Size: 22 1220
MslI CAynn'nnrTG
Cuts at: 0 601 1242
Size: 601 641
MspA1I CmG'CkG
Cuts at: 0 1105 1242
Size: 1105 137
NcoI C'CATG_G
Cuts at: 0 555 1130 1242
Size: 555 575 112
Fragments arranged by size:
575 555 112
PfoI T'CCnGG_A
Cuts at: 0 776 1242
Size: 776 466
PpuMI rG'GwC_Cy
Cuts at: 0 384 1242
Size: 384 858
PsiI TTA'TAA
Cuts at: 0 89 1242
Size: 89 1153
PstI C_TGCA'G
Cuts at: 0 434 1242
Size: 434 808
SapI GCTCTTCn'nnn_
Cuts at: 0 1213 1242
Size: 1213 29
SfcI C'TryA_G
Cuts at: 0 239 430 1242
Size: 239 191 812
Fragments arranged by size:
812 239 191
SmlI C'TyrA_G
Cuts at: 0 178 228 1242
Size: 178 50 1014
Fragments arranged by size:
1014 178 50
StuI AGG'CCT
Cuts at: 0 17 1242
Size: 17 1225
StyI C'CwwG_G
Cuts at: 0 79 108 134 555 1130 1242
Size: 79 29 26 421 575 112
Fragments arranged by size:
575 421 112 79 29 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 1242
Size: 253 989
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 848 880 1242
Size: 848 32 362
Fragments arranged by size:
848 362 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 258 1242
Size: 15 243 984
Fragments arranged by size:
984 243 15
XmnI GAAnn'nnTTC
Cuts at: 0 214 1242
Size: 214 1028
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaLI ApoI
BanI BbsI BglII BlpI Bme1580I BmgBI BpmI BpuEI
BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspHI BspMI BssSI
BstXI BstYI BtgI BtgZI EaeI EarI Eco57MI EcoO109I
FalI Hin4I HincII HindIII HpaI MscI MslI MspA1I
NcoI PfoI PpuMI PsiI PstI SapI SfcI SmlI
StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmaI ZraI