Restriction map for
pFN21AE4390
(Linear) (Six-base) MAPSORT of: KIEE4390.seq Check: 2074 from: 1 to: 1236
KIEE4390.orf
With 182 enzymes: SgfI *
March 22, 2012 17:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1236
Size: 1021 215
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1236
Size: 179 32 1025
Fragments arranged by size:
1025 179 32
AlwNI CAG_nnn'CTG
Cuts at: 0 493 837 1098 1197 1236
Size: 493 344 261 99 39
Fragments arranged by size:
493 344 261 99 39
ApaI G_GGCC'C
Cuts at: 0 34 377 1236
Size: 34 343 859
Fragments arranged by size:
859 343 34
AvaI C'yCGr_G
Cuts at: 0 20 27 103 253 509 1236
Size: 20 7 76 150 256 727
Fragments arranged by size:
727 256 150 76 20 7
AvrII C'CTAG_G
Cuts at: 0 55 1236
Size: 55 1181
BanI G'GyrC_C
Cuts at: 0 903 1177 1236
Size: 903 274 59
Fragments arranged by size:
903 274 59
BanII G_rGCy'C
Cuts at: 0 34 48 377 612 665 706 1236
Size: 34 14 329 235 53 41 530
Fragments arranged by size:
530 329 235 53 41 34 14
BbeI G_GCGC'C
Cuts at: 0 907 1236
Size: 907 329
BbsI GAAGACnn'nnnn_
Cuts at: 0 689 1236
Size: 689 547
BbvCI CC'TCA_GC
Cuts at: 0 460 1236
Size: 460 776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 804 838 879 913 1236
Size: 804 34 41 34 323
Fragments arranged by size:
804 323 41 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 571 818 832 901 1048 1060 1236
Size: 571 247 14 69 147 12 176
Fragments arranged by size:
571 247 176 147 69 14 12
BglII A'GATC_T
Cuts at: 0 1166 1236
Size: 1166 70
Bme1580I G_kGCm'C
Cuts at: 0 34 254 377 972 1180 1236
Size: 34 220 123 595 208 56
Fragments arranged by size:
595 220 208 123 56 34
BmgBI CAC'GTC
Cuts at: 0 249 1236
Size: 249 987
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 540 1236
Size: 540 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 904 982 1236
Size: 109 795 78 254
Fragments arranged by size:
795 254 109 78
Bpu10I CC'TnA_GC
Cuts at: 0 206 460 1219 1236
Size: 206 254 759 17
Fragments arranged by size:
759 254 206 17
BsaAI yAC'GTr
Cuts at: 0 1116 1236
Size: 1116 120
BsaHI Gr'CG_yC
Cuts at: 0 904 1151 1236
Size: 904 247 85
Fragments arranged by size:
904 247 85
BsaWI w'CCGG_w
Cuts at: 0 334 1229 1236
Size: 334 895 7
Fragments arranged by size:
895 334 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 322 1236
Size: 271 51 914
Fragments arranged by size:
914 271 51
BseYI C'CCAG_C
Cuts at: 0 198 690 1236
Size: 198 492 546
Fragments arranged by size:
546 492 198
BsiEI CG_ry'CG
Cuts at: 0 1070 1236
Size: 1070 166
BsiHKAI G_wGCw'C
Cuts at: 0 48 1236
Size: 48 1188
Bsp1286I G_dGCh'C
Cuts at: 0 34 48 254 377 612 665 706 972
Size: 34 14 206 123 235 53 41 266
Cuts at: 972 1180 1236
Size: 208 56
Fragments arranged by size:
266 235 208 206 123 56 53 41
34 14
BspHI T'CATG_A
Cuts at: 0 344 1236
Size: 344 892
BsrBI CCG'CTC
Cuts at: 0 1137 1236
Size: 1137 99
BsrDI GCAATG_nn'
Cuts at: 0 164 842 1236
Size: 164 678 394
Fragments arranged by size:
678 394 164
BsrFI r'CCGG_y
Cuts at: 0 1128 1236
Size: 1128 108
BstAPI GCAn_nnn'nTGC
Cuts at: 0 493 1236
Size: 493 743
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 1236
Size: 303 933
BstYI r'GATC_y
Cuts at: 0 1094 1166 1236
Size: 1094 72 70
Fragments arranged by size:
1094 72 70
BtgI C'CryG_G
Cuts at: 0 242 302 318 584 710 1037 1236
Size: 242 60 16 266 126 327 199
Fragments arranged by size:
327 266 242 199 126 60 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 282 807 1236
Size: 282 525 429
Fragments arranged by size:
525 429 282
BtsI GCAGTG_nn'
Cuts at: 0 486 1236
Size: 486 750
EaeI y'GGCC_r
Cuts at: 0 2 239 713 1040 1049 1236
Size: 2 237 474 327 9 187
Fragments arranged by size:
474 327 237 187 9 2
EarI CTCTTCn'nnn_
Cuts at: 0 199 1236
Size: 199 1037
EcoICRI GAG'CTC
Cuts at: 0 46 1236
Size: 46 1190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 904 982 1021 1236
Size: 109 795 78 39 215
Fragments arranged by size:
795 215 109 78 39
EcoO109I rG'GnC_Cy
Cuts at: 0 31 59 373 834 1236
Size: 31 28 314 461 402
Fragments arranged by size:
461 402 314 31 28
HaeII r_GCGC'y
Cuts at: 0 907 1236
Size: 907 329
HincII GTy'rAC
Cuts at: 0 354 1236
Size: 354 882
KasI G'GCGC_C
Cuts at: 0 903 1236
Size: 903 333
MscI TGG'CCA
Cuts at: 0 241 1042 1236
Size: 241 801 194
Fragments arranged by size:
801 241 194
MslI CAynn'nnrTG
Cuts at: 0 247 301 1236
Size: 247 54 935
Fragments arranged by size:
935 247 54
MspA1I CmG'CkG
Cuts at: 0 202 320 1194 1236
Size: 202 118 874 42
Fragments arranged by size:
874 202 118 42
NaeI GCC'GGC
Cuts at: 0 1130 1236
Size: 1130 106
NarI GG'CG_CC
Cuts at: 0 904 1236
Size: 904 332
NcoI C'CATG_G
Cuts at: 0 242 302 1037 1236
Size: 242 60 735 199
Fragments arranged by size:
735 242 199 60
NgoMIV G'CCGG_C
Cuts at: 0 1128 1236
Size: 1128 108
NspI r_CATG'y
Cuts at: 0 134 1236
Size: 134 1102
PasI CC'CwG_GG
Cuts at: 0 837 1236
Size: 837 399
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 551 566 658 837 1098 1197 1236
Size: 93 458 15 92 179 261 99 39
Fragments arranged by size:
458 261 179 99 93 92 39 15
PspOMI G'GGCC_C
Cuts at: 0 30 373 1236
Size: 30 343 863
Fragments arranged by size:
863 343 30
SacI G_AGCT'C
Cuts at: 0 48 1236
Size: 48 1188
SacII CC_GC'GG
Cuts at: 0 321 1236
Size: 321 915
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1048 1236
Size: 1048 188
SfoI GGC'GCC
Cuts at: 0 905 1236
Size: 905 331
SmaI CCC'GGG
Cuts at: 0 22 511 1236
Size: 22 489 725
Fragments arranged by size:
725 489 22
SrfI GCCC'GGGC
Cuts at: 0 22 511 1236
Size: 22 489 725
Fragments arranged by size:
725 489 22
StyI C'CwwG_G
Cuts at: 0 55 242 302 395 746 1037 1236
Size: 55 187 60 93 351 291 199
Fragments arranged by size:
351 291 199 187 93 60 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 1236
Size: 465 32 739
Fragments arranged by size:
739 465 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 1044 1095 1236
Size: 798 246 51 141
Fragments arranged by size:
798 246 141 51
XmaI C'CCGG_G
Cuts at: 0 20 509 1236
Size: 20 489 727
Fragments arranged by size:
727 489 20
XmnI GAAnn'nnTTC
Cuts at: 0 1018 1236
Size: 1018 218
Enzymes that do cut:
AcuI AloI AlwNI ApaI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI
BplI BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII HincII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PspOMI
SacI SacII SfiI SfoI SmaI SrfI StyI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BciVI BclI BfrBI BlpI BmrI
BmtI BpuEI BsaI BsaBI BsaXI BsgI BsiWI BsmI
BsmBI BspEI BspMI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
ZraI