Restriction map for
pFN21AE4387
(Linear) (Six-base) MAPSORT of: KIEE4387.seq Check: 3900 from: 1 to: 1224
KIEE4387.orf
With 182 enzymes: SgfI *
March 22, 2012 17:53 ..
AccI GT'mk_AC
Cuts at: 0 512 1224
Size: 512 712
Acc65I G'GTAC_C
Cuts at: 0 728 1224
Size: 728 496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 1070 1224
Size: 795 275 154
Fragments arranged by size:
795 275 154
AleI CACnn'nnGTG
Cuts at: 0 1040 1224
Size: 1040 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 838 1224
Size: 838 386
AlwNI CAG_nnn'CTG
Cuts at: 0 879 1164 1224
Size: 879 285 60
Fragments arranged by size:
879 285 60
ApaI G_GGCC'C
Cuts at: 0 1118 1224
Size: 1118 106
ApoI r'AATT_y
Cuts at: 0 280 1060 1224
Size: 280 780 164
Fragments arranged by size:
780 280 164
BanI G'GyrC_C
Cuts at: 0 298 365 450 728 988 1224
Size: 298 67 85 278 260 236
Fragments arranged by size:
298 278 260 236 85 67
BanII G_rGCy'C
Cuts at: 0 1118 1224
Size: 1118 106
BbeI G_GCGC'C
Cuts at: 0 369 1224
Size: 369 855
BbsI GAAGACnn'nnnn_
Cuts at: 0 612 1224
Size: 612 612
BbvCI CC'TCA_GC
Cuts at: 0 872 1224
Size: 872 352
BclI T'GATC_A
Cuts at: 0 35 179 657 1224
Size: 35 144 478 567
Fragments arranged by size:
567 478 144 35
Bme1580I G_kGCm'C
Cuts at: 0 303 455 991 1118 1224
Size: 303 152 536 127 106
Fragments arranged by size:
536 303 152 127 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1003 1224
Size: 961 42 221
Fragments arranged by size:
961 221 42
Bpu10I CC'TnA_GC
Cuts at: 0 872 1224
Size: 872 352
BsaHI Gr'CG_yC
Cuts at: 0 366 1224
Size: 366 858
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 389 419 1224
Size: 389 30 805
Fragments arranged by size:
805 389 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 1224
Size: 397 827
BseYI C'CCAG_C
Cuts at: 0 57 1224
Size: 57 1167
BsmI GAATG_Cn'
Cuts at: 0 265 1224
Size: 265 959
BsmBI CGTCTCn'nnnn_
Cuts at: 0 54 1224
Size: 54 1170
Bsp1286I G_dGCh'C
Cuts at: 0 303 455 991 1118 1224
Size: 303 152 536 127 106
Fragments arranged by size:
536 303 152 127 106
BspHI T'CATG_A
Cuts at: 0 680 1224
Size: 680 544
BsrDI GCAATG_nn'
Cuts at: 0 107 1138 1224
Size: 107 1031 86
Fragments arranged by size:
1031 107 86
BssHII G'CGCG_C
Cuts at: 0 115 1224
Size: 115 1109
BstYI r'GATC_y
Cuts at: 0 158 1160 1224
Size: 158 1002 64
Fragments arranged by size:
1002 158 64
BtgI C'CryG_G
Cuts at: 0 788 1224
Size: 788 436
BtsI GCAGTG_nn'
Cuts at: 0 262 536 865 1176 1224
Size: 262 274 329 311 48
Fragments arranged by size:
329 311 274 262 48
DraI TTT'AAA
Cuts at: 0 1001 1224
Size: 1001 223
EaeI y'GGCC_r
Cuts at: 0 68 585 1224
Size: 68 517 639
Fragments arranged by size:
639 517 68
EarI CTCTTCn'nnn_
Cuts at: 0 214 1216 1224
Size: 214 1002 8
Fragments arranged by size:
1002 214 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 454 1224
Size: 454 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 961 1003 1070 1224
Size: 795 166 42 67 154
Fragments arranged by size:
795 166 154 67 42
EcoO109I rG'GnC_Cy
Cuts at: 0 132 1224
Size: 132 1092
EcoRI G'AATT_C
Cuts at: 0 1060 1224
Size: 1060 164
EcoRV GAT'ATC
Cuts at: 0 1195 1224
Size: 1195 29
HaeII r_GCGC'y
Cuts at: 0 369 1224
Size: 369 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 908 940 1184 1216 1224
Size: 143 32 733 32 244 32 8
Fragments arranged by size:
733 244 143 32 32 32 8
KasI G'GCGC_C
Cuts at: 0 365 1224
Size: 365 859
KpnI G_GTAC'C
Cuts at: 0 732 1224
Size: 732 492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1224
Size: 617 607
MscI TGG'CCA
Cuts at: 0 70 587 1224
Size: 70 517 637
Fragments arranged by size:
637 517 70
MslI CAynn'nnrTG
Cuts at: 0 448 1040 1224
Size: 448 592 184
Fragments arranged by size:
592 448 184
MspA1I CmG'CkG
Cuts at: 0 30 185 391 1023 1224
Size: 30 155 206 632 201
Fragments arranged by size:
632 206 201 155 30
NarI GG'CG_CC
Cuts at: 0 366 1224
Size: 366 858
PasI CC'CwG_GG
Cuts at: 0 171 1224
Size: 171 1053
PpuMI rG'GwC_Cy
Cuts at: 0 132 1224
Size: 132 1092
PspOMI G'GGCC_C
Cuts at: 0 1114 1224
Size: 1114 110
PstI C_TGCA'G
Cuts at: 0 1160 1224
Size: 1160 64
PvuII CAG'CTG
Cuts at: 0 391 1023 1224
Size: 391 632 201
Fragments arranged by size:
632 391 201
SexAI A'CCwGG_T
Cuts at: 0 626 1224
Size: 626 598
SfcI C'TryA_G
Cuts at: 0 386 1156 1224
Size: 386 770 68
Fragments arranged by size:
770 386 68
SfoI GGC'GCC
Cuts at: 0 367 1224
Size: 367 857
SpeI A'CTAG_T
Cuts at: 0 594 1224
Size: 594 630
StyI C'CwwG_G
Cuts at: 0 1110 1224
Size: 1110 114
TatI w'GTAC_w
Cuts at: 0 348 503 1032 1224
Size: 348 155 529 192
Fragments arranged by size:
529 348 192 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 1224
Size: 389 32 803
Fragments arranged by size:
803 389 32
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI
BanI BanII BbeI BbsI BbvCI BclI Bme1580I BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I
BspHI BsrDI BssHII BstYI BtgI BtsI DraI EaeI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NarI PasI
PpuMI PspOMI PstI PvuII SexAI SfcI SfoI SpeI
StyI TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI
BsgI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI