Restriction map for
pFN21AE4385
(Linear) (Six-base) MAPSORT of: KIEE4385.seq Check: 4367 from: 1 to: 1218
KIEE4385.orf
With 182 enzymes: SgfI *
March 22, 2012 17:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 536 1218
Size: 536 682
AccI GT'mk_AC
Cuts at: 0 782 1218
Size: 782 436
Acc65I G'GTAC_C
Cuts at: 0 806 1218
Size: 806 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 598 1218
Size: 37 561 620
Fragments arranged by size:
620 561 37
AfeI AGC'GCT
Cuts at: 0 724 1218
Size: 724 494
AflIII A'CryG_T
Cuts at: 0 1052 1218
Size: 1052 166
AlwNI CAG_nnn'CTG
Cuts at: 0 35 1218
Size: 35 1183
ApoI r'AATT_y
Cuts at: 0 130 1128 1218
Size: 130 998 90
Fragments arranged by size:
998 130 90
AvrII C'CTAG_G
Cuts at: 0 319 1218
Size: 319 899
BanI G'GyrC_C
Cuts at: 0 639 806 1099 1218
Size: 639 167 293 119
Fragments arranged by size:
639 293 167 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 585 619 1218
Size: 585 34 599
Fragments arranged by size:
599 585 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 198 459 1218
Size: 198 261 759
Fragments arranged by size:
759 261 198
BclI T'GATC_A
Cuts at: 0 609 1218
Size: 609 609
BglII A'GATC_T
Cuts at: 0 332 1218
Size: 332 886
Bme1580I G_kGCm'C
Cuts at: 0 642 673 1156 1218
Size: 642 31 483 62
Fragments arranged by size:
642 483 62 31
BmgBI CAC'GTC
Cuts at: 0 1053 1218
Size: 1053 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 1218
Size: 174 1044
Bpu10I CC'TnA_GC
Cuts at: 0 349 1218
Size: 349 869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 871 1218
Size: 177 694 347
Fragments arranged by size:
694 347 177
BsaWI w'CCGG_w
Cuts at: 0 816 1202 1218
Size: 816 386 16
Fragments arranged by size:
816 386 16
BseYI C'CCAG_C
Cuts at: 0 532 1218
Size: 532 686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 844 1074 1218
Size: 428 416 230 144
Fragments arranged by size:
428 416 230 144
Bsp1286I G_dGCh'C
Cuts at: 0 642 673 1156 1218
Size: 642 31 483 62
Fragments arranged by size:
642 483 62 31
BspEI T'CCGG_A
Cuts at: 0 816 1202 1218
Size: 816 386 16
Fragments arranged by size:
816 386 16
BspHI T'CATG_A
Cuts at: 0 612 989 1218
Size: 612 377 229
Fragments arranged by size:
612 377 229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1181 1218
Size: 536 645 37
Fragments arranged by size:
645 536 37
BsrGI T'GTAC_A
Cuts at: 0 743 1218
Size: 743 475
BstEII G'GTnAC_C
Cuts at: 0 708 1218
Size: 708 510
BstYI r'GATC_y
Cuts at: 0 332 571 1218
Size: 332 239 647
Fragments arranged by size:
647 332 239
BstZ17I GTA'TAC
Cuts at: 0 783 1218
Size: 783 435
Bsu36I CC'TnA_GG
Cuts at: 0 625 1218
Size: 625 593
BtgI C'CryG_G
Cuts at: 0 164 452 1218
Size: 164 288 766
Fragments arranged by size:
766 288 164
BtsI GCAGTG_nn'
Cuts at: 0 1189 1212 1218
Size: 1189 23 6
Fragments arranged by size:
1189 23 6
ClaI AT'CG_AT
Cuts at: 0 605 1218
Size: 605 613
DraI TTT'AAA
Cuts at: 0 114 1218
Size: 114 1104
EaeI y'GGCC_r
Cuts at: 0 161 1218
Size: 161 1057
EarI CTCTTCn'nnn_
Cuts at: 0 306 935 1218
Size: 306 629 283
Fragments arranged by size:
629 306 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 598 1218
Size: 37 561 620
Fragments arranged by size:
620 561 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 1218
Size: 253 965
EcoO109I rG'GnC_Cy
Cuts at: 0 207 414 771 1218
Size: 207 207 357 447
Fragments arranged by size:
447 357 207 207
EcoRI G'AATT_C
Cuts at: 0 130 1218
Size: 130 1088
EcoRV GAT'ATC
Cuts at: 0 795 1218
Size: 795 423
HaeII r_GCGC'y
Cuts at: 0 726 1218
Size: 726 492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 380 412 684 716 1218
Size: 166 32 182 32 272 32 502
Fragments arranged by size:
502 272 182 166 32 32 32
KpnI G_GTAC'C
Cuts at: 0 810 1218
Size: 810 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 1218
Size: 681 537
MscI TGG'CCA
Cuts at: 0 163 1218
Size: 163 1055
MslI CAynn'nnrTG
Cuts at: 0 70 443 988 991 1218
Size: 70 373 545 3 227
Fragments arranged by size:
545 373 227 70 3
MspA1I CmG'CkG
Cuts at: 0 32 568 1113 1218
Size: 32 536 545 105
Fragments arranged by size:
545 536 105 32
NcoI C'CATG_G
Cuts at: 0 164 452 1218
Size: 164 288 766
Fragments arranged by size:
766 288 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 967 1218
Size: 967 251
PfoI T'CCnGG_A
Cuts at: 0 431 1218
Size: 431 787
PpuMI rG'GwC_Cy
Cuts at: 0 207 771 1218
Size: 207 564 447
Fragments arranged by size:
564 447 207
PsiI TTA'TAA
Cuts at: 0 824 1218
Size: 824 394
PvuII CAG'CTG
Cuts at: 0 32 1218
Size: 32 1186
SanDI GG'GwC_CC
Cuts at: 0 771 1218
Size: 771 447
SapI GCTCTTCn'nnn_
Cuts at: 0 306 1218
Size: 306 912
SexAI A'CCwGG_T
Cuts at: 0 1007 1218
Size: 1007 211
SmlI C'TyrA_G
Cuts at: 0 192 886 1218
Size: 192 694 332
Fragments arranged by size:
694 332 192
StuI AGG'CCT
Cuts at: 0 248 271 1218
Size: 248 23 947
Fragments arranged by size:
947 248 23
StyI C'CwwG_G
Cuts at: 0 125 164 254 319 452 509 527 538
Size: 125 39 90 65 133 57 18 11
Cuts at: 538 901 1218
Size: 363 317
Fragments arranged by size:
363 317 133 125 90 65 57 39
18 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 217 657 1087 1218
Size: 217 440 430 131
Fragments arranged by size:
440 430 217 131
TatI w'GTAC_w
Cuts at: 0 362 743 1218
Size: 362 381 475
Fragments arranged by size:
475 381 362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 456 488 1218
Size: 456 32 730
Fragments arranged by size:
730 456 32
Tth111I GACn'n_nGTC
Cuts at: 0 619 1218
Size: 619 599
XmnI GAAnn'nnTTC
Cuts at: 0 25 724 944 1075 1218
Size: 25 699 220 131 143
Fragments arranged by size:
699 220 143 131 25
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlwNI ApoI
AvrII BanI BcgI BciVI BclI BglII Bme1580I BmgBI
BmrI Bpu10I BpuEI BsaWI BseYI BsgI Bsp1286I BspEI
BspHI BspMI BsrGI BstEII BstYI BstZ17I Bsu36I BtgI
BtsI ClaI DraI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I KpnI MmeI MscI MslI
MspA1I NcoI PflMI PfoI PpuMI PsiI PvuII SanDI
SapI SexAI SmlI StuI StyI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI BaeI BamHI
BanII BbeI BbsI BbvCI BfrBI BglI BlpI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BsiEI BsiHKAI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BtgZI CspCI DraIII
DrdI EagI EciI EcoICRI FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI
XmaI ZraI