Restriction map for
pFN21AE4383
(Linear) (Six-base) MAPSORT of: KIEE4383.seq Check: 536 from: 1 to: 1212
KIEE4383.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AccI GT'mk_AC
Cuts at: 0 185 1212
Size: 185 1027
Acc65I G'GTAC_C
Cuts at: 0 1142 1212
Size: 1142 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1212
Size: 737 475
AlwNI CAG_nnn'CTG
Cuts at: 0 523 1212
Size: 523 689
ApaLI G'TGCA_C
Cuts at: 0 365 1212
Size: 365 847
ApoI r'AATT_y
Cuts at: 0 289 412 736 854 926 1051 1189 1212
Size: 289 123 324 118 72 125 138 23
Fragments arranged by size:
324 289 138 125 123 118 72 23
AseI AT'TA_AT
Cuts at: 0 260 757 1212
Size: 260 497 455
Fragments arranged by size:
497 455 260
BamHI G'GATC_C
Cuts at: 0 583 1212
Size: 583 629
BanI G'GyrC_C
Cuts at: 0 1142 1212
Size: 1142 70
BclI T'GATC_A
Cuts at: 0 11 627 1212
Size: 11 616 585
Fragments arranged by size:
616 585 11
Bme1580I G_kGCm'C
Cuts at: 0 369 1212
Size: 369 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1212
Size: 926 286
BsaI GGTCTCn'nnnn_
Cuts at: 0 976 1103 1212
Size: 976 127 109
Fragments arranged by size:
976 127 109
BsaWI w'CCGG_w
Cuts at: 0 985 1212
Size: 985 227
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 461 934 1193 1212
Size: 461 473 259 19
Fragments arranged by size:
473 461 259 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 550 1212
Size: 391 159 662
Fragments arranged by size:
662 391 159
BsiHKAI G_wGCw'C
Cuts at: 0 369 1212
Size: 369 843
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1175 1212
Size: 1175 37
Bsp1286I G_dGCh'C
Cuts at: 0 369 1212
Size: 369 843
BsrDI GCAATG_nn'
Cuts at: 0 900 1212
Size: 900 312
BsrGI T'GTAC_A
Cuts at: 0 132 1212
Size: 132 1080
BssSI C'ACGA_G
Cuts at: 0 38 92 1212
Size: 38 54 1120
Fragments arranged by size:
1120 54 38
BstAPI GCAn_nnn'nTGC
Cuts at: 0 523 1212
Size: 523 689
BstYI r'GATC_y
Cuts at: 0 423 583 1212
Size: 423 160 629
Fragments arranged by size:
629 423 160
BstZ17I GTA'TAC
Cuts at: 0 186 1212
Size: 186 1026
DraI TTT'AAA
Cuts at: 0 597 653 1212
Size: 597 56 559
Fragments arranged by size:
597 559 56
EarI CTCTTCn'nnn_
Cuts at: 0 911 1212
Size: 911 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 926 1212
Size: 737 189 286
Fragments arranged by size:
737 286 189
EcoRV GAT'ATC
Cuts at: 0 5 1212
Size: 5 1207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 1212
Size: 3 32 1177
Fragments arranged by size:
1177 32 3
HincII GTy'rAC
Cuts at: 0 231 1212
Size: 231 981
KpnI G_GTAC'C
Cuts at: 0 1146 1212
Size: 1146 66
MfeI C'AATT_G
Cuts at: 0 197 1212
Size: 197 1015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1004 1046 1212
Size: 752 252 42 166
Fragments arranged by size:
752 252 166 42
MslI CAynn'nnrTG
Cuts at: 0 500 1212
Size: 500 712
MspA1I CmG'CkG
Cuts at: 0 534 589 1212
Size: 534 55 623
Fragments arranged by size:
623 534 55
NspI r_CATG'y
Cuts at: 0 1017 1212
Size: 1017 195
PflMI CCAn_nnn'nTGG
Cuts at: 0 1164 1212
Size: 1164 48
PsiI TTA'TAA
Cuts at: 0 155 1212
Size: 155 1057
PvuII CAG'CTG
Cuts at: 0 534 1212
Size: 534 678
ScaI AGT'ACT
Cuts at: 0 802 1212
Size: 802 410
SfcI C'TryA_G
Cuts at: 0 848 1212
Size: 848 364
SphI G_CATG'C
Cuts at: 0 1017 1212
Size: 1017 195
StyI C'CwwG_G
Cuts at: 0 384 906 1173 1212
Size: 384 522 267 39
Fragments arranged by size:
522 384 267 39
TatI w'GTAC_w
Cuts at: 0 132 800 1212
Size: 132 668 412
Fragments arranged by size:
668 412 132
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaLI ApoI AseI BamHI
BanI BclI Bme1580I BpmI BsaI BsaWI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BsrDI BsrGI BssSI BstAPI BstYI
BstZ17I DraI EarI Eco57MI EcoRV Hin4I HincII KpnI
MfeI MmeI MslI MspA1I NspI PflMI PsiI PvuII
ScaI SfcI SphI StyI TatI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrFI BssHII BstBI
BstEII BstXI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI