Restriction map for
pFN21AE4381
(Linear) (Six-base) MAPSORT of: KIEE4381.seq Check: 7159 from: 1 to: 1203
KIEE4381.orf
With 182 enzymes: SgfI *
March 22, 2012 17:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 922 1203
Size: 407 515 281
Fragments arranged by size:
515 407 281
AflIII A'CryG_T
Cuts at: 0 624 1137 1203
Size: 624 513 66
Fragments arranged by size:
624 513 66
AgeI A'CCGG_T
Cuts at: 0 729 1203
Size: 729 474
AleI CACnn'nnGTG
Cuts at: 0 903 1203
Size: 903 300
AlwNI CAG_nnn'CTG
Cuts at: 0 540 1203
Size: 540 663
ApoI r'AATT_y
Cuts at: 0 115 290 1203
Size: 115 175 913
Fragments arranged by size:
913 175 115
AvaI C'yCGr_G
Cuts at: 0 107 547 663 1203
Size: 107 440 116 540
Fragments arranged by size:
540 440 116 107
BanI G'GyrC_C
Cuts at: 0 658 876 1031 1105 1203
Size: 658 218 155 74 98
Fragments arranged by size:
658 218 155 98 74
BanII G_rGCy'C
Cuts at: 0 79 529 797 1203
Size: 79 450 268 406
Fragments arranged by size:
450 406 268 79
BbvCI CC'TCA_GC
Cuts at: 0 164 1013 1203
Size: 164 849 190
Fragments arranged by size:
849 190 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 386 1203
Size: 352 34 817
Fragments arranged by size:
817 352 34
Bme1580I G_kGCm'C
Cuts at: 0 661 1203
Size: 661 542
BmrI ACTGGGnnnn_n'
Cuts at: 0 471 663 1203
Size: 471 192 540
Fragments arranged by size:
540 471 192
BmtI G_CTAG'C
Cuts at: 0 1021 1203
Size: 1021 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 517 1203
Size: 444 73 686
Fragments arranged by size:
686 444 73
Bpu10I CC'TnA_GC
Cuts at: 0 164 1013 1203
Size: 164 849 190
Fragments arranged by size:
849 190 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 836 1203
Size: 836 367
BsaBI GATnn'nnATC
Cuts at: 0 322 1203
Size: 322 881
BsaWI w'CCGG_w
Cuts at: 0 579 729 1203
Size: 579 150 474
Fragments arranged by size:
579 474 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 420 450 816 846 1203
Size: 420 30 366 30 357
Fragments arranged by size:
420 366 357 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 441 1203
Size: 441 762
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1203
Size: 3 1200
BsiEI CG_ry'CG
Cuts at: 0 128 580 1203
Size: 128 452 623
Fragments arranged by size:
623 452 128
BsiHKAI G_wGCw'C
Cuts at: 0 29 79 797 1064 1203
Size: 29 50 718 267 139
Fragments arranged by size:
718 267 139 50 29
Bsp1286I G_dGCh'C
Cuts at: 0 29 79 529 661 797 1064 1203
Size: 29 50 450 132 136 267 139
Fragments arranged by size:
450 267 139 136 132 50 29
BsrDI GCAATG_nn'
Cuts at: 0 827 1203
Size: 827 376
BsrFI r'CCGG_y
Cuts at: 0 729 1203
Size: 729 474
BssHII G'CGCG_C
Cuts at: 0 139 1203
Size: 139 1064
BtgI C'CryG_G
Cuts at: 0 596 1203
Size: 596 607
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 309 661 1203
Size: 120 189 352 542
Fragments arranged by size:
542 352 189 120
EarI CTCTTCn'nnn_
Cuts at: 0 31 1203
Size: 31 1172
EcoICRI GAG'CTC
Cuts at: 0 77 795 1203
Size: 77 718 408
Fragments arranged by size:
718 408 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 444 517 922 1203
Size: 407 37 73 405 281
Fragments arranged by size:
407 405 281 73 37
FspI TGC'GCA
Cuts at: 0 1168 1203
Size: 1168 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 816 848 1183 1203
Size: 420 32 364 32 335 20
Fragments arranged by size:
420 364 335 32 32 20
HincII GTy'rAC
Cuts at: 0 345 1203
Size: 345 858
HpaI GTT'AAC
Cuts at: 0 345 1203
Size: 345 858
MfeI C'AATT_G
Cuts at: 0 983 1203
Size: 983 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1203
Size: 22 1181
MslI CAynn'nnrTG
Cuts at: 0 595 646 903 1203
Size: 595 51 257 300
Fragments arranged by size:
595 300 257 51
MspA1I CmG'CkG
Cuts at: 0 786 1203
Size: 786 417
NcoI C'CATG_G
Cuts at: 0 596 1203
Size: 596 607
NheI G'CTAG_C
Cuts at: 0 1017 1203
Size: 1017 186
NspI r_CATG'y
Cuts at: 0 628 1141 1203
Size: 628 513 62
Fragments arranged by size:
628 513 62
PciI A'CATG_T
Cuts at: 0 624 1137 1203
Size: 624 513 66
Fragments arranged by size:
624 513 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1203
Size: 642 561
PspXI vC'TCGA_Gb
Cuts at: 0 547 1203
Size: 547 656
PstI C_TGCA'G
Cuts at: 0 545 1203
Size: 545 658
PvuII CAG'CTG
Cuts at: 0 786 1203
Size: 786 417
SacI G_AGCT'C
Cuts at: 0 79 797 1203
Size: 79 718 406
Fragments arranged by size:
718 406 79
SfcI C'TryA_G
Cuts at: 0 541 701 1179 1194 1203
Size: 541 160 478 15 9
Fragments arranged by size:
541 478 160 15 9
SmaI CCC'GGG
Cuts at: 0 109 1203
Size: 109 1094
SmlI C'TyrA_G
Cuts at: 0 547 663 1203
Size: 547 116 540
Fragments arranged by size:
547 540 116
SrfI GCCC'GGGC
Cuts at: 0 109 1203
Size: 109 1094
StyI C'CwwG_G
Cuts at: 0 596 636 1101 1203
Size: 596 40 465 102
Fragments arranged by size:
596 465 102 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 71 261 1203
Size: 71 190 942
Fragments arranged by size:
942 190 71
TatI w'GTAC_w
Cuts at: 0 650 1203
Size: 650 553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 860 892 1203
Size: 257 32 571 32 311
Fragments arranged by size:
571 311 257 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 828 1203
Size: 828 375
XhoI C'TCGA_G
Cuts at: 0 547 663 1203
Size: 547 116 540
Fragments arranged by size:
547 540 116
XmaI C'CCGG_G
Cuts at: 0 107 1203
Size: 107 1096
XmnI GAAnn'nnTTC
Cuts at: 0 392 1203
Size: 392 811
Enzymes that do cut:
AcuI AflIII AgeI AleI AlwNI ApoI AvaI BanI
BanII BbvCI BcgI Bme1580I BmrI BmtI BpmI Bpu10I
BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
Bsp1286I BsrDI BsrFI BssHII BtgI BtgZI EarI EcoICRI
Eco57MI FspI Hin4I HincII HpaI MfeI MmeI MslI
MspA1I NcoI NheI NspI PciI PflMI PspXI PstI
PvuII SacI SfcI SmaI SmlI SrfI StyI TaqII
TatI TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BplI BpuEI BsaAI BsaHI BseYI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI
HaeII HindIII KasI KpnI MluI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI XbaI XcmI ZraI