Restriction map for
pFN21AE4380
(Linear) (Six-base) MAPSORT of: KIEE4380.seq Check: 4109 from: 1 to: 1200
KIEE4380.orf
With 182 enzymes: SgfI *
March 22, 2012 17:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 819 1200
Size: 819 381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1200
Size: 211 989
AleI CACnn'nnGTG
Cuts at: 0 652 1200
Size: 652 548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1016 1200
Size: 1016 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 1200
Size: 433 32 735
Fragments arranged by size:
735 433 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1029 1075 1200
Size: 1029 46 125
Fragments arranged by size:
1029 125 46
ApaI G_GGCC'C
Cuts at: 0 949 1200
Size: 949 251
ApoI r'AATT_y
Cuts at: 0 1161 1200
Size: 1161 39
BanI G'GyrC_C
Cuts at: 0 178 654 1200
Size: 178 476 546
Fragments arranged by size:
546 476 178
BanII G_rGCy'C
Cuts at: 0 106 949 1200
Size: 106 843 251
Fragments arranged by size:
843 251 106
BbeI G_GCGC'C
Cuts at: 0 182 1200
Size: 182 1018
BbsI GAAGACnn'nnnn_
Cuts at: 0 845 1200
Size: 845 355
BbvCI CC'TCA_GC
Cuts at: 0 614 1200
Size: 614 586
BclI T'GATC_A
Cuts at: 0 1170 1200
Size: 1170 30
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1200
Size: 61 1139
Bme1580I G_kGCm'C
Cuts at: 0 949 1200
Size: 949 251
BmrI ACTGGGnnnn_n'
Cuts at: 0 704 892 1200
Size: 704 188 308
Fragments arranged by size:
704 308 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 238 292 397 460 1200
Size: 106 132 54 105 63 740
Fragments arranged by size:
740 132 106 105 63 54
Bpu10I CC'TnA_GC
Cuts at: 0 463 614 950 1200
Size: 463 151 336 250
Fragments arranged by size:
463 336 250 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 554 950 1200
Size: 554 396 250
Fragments arranged by size:
554 396 250
BsaHI Gr'CG_yC
Cuts at: 0 18 179 1200
Size: 18 161 1021
Fragments arranged by size:
1021 161 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 578 608 1200
Size: 433 30 115 30 592
Fragments arranged by size:
592 433 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 799 1000 1003 1049 1200
Size: 523 276 201 3 46 151
Fragments arranged by size:
523 276 201 151 46 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1200
Size: 922 278
BsiHKAI G_wGCw'C
Cuts at: 0 106 1200
Size: 106 1094
Bsp1286I G_dGCh'C
Cuts at: 0 106 949 1200
Size: 106 843 251
Fragments arranged by size:
843 251 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 819 1200
Size: 819 381
BssSI C'ACGA_G
Cuts at: 0 43 1200
Size: 43 1157
BstEII G'GTnAC_C
Cuts at: 0 525 1200
Size: 525 675
BstYI r'GATC_y
Cuts at: 0 767 1200
Size: 767 433
BtgI C'CryG_G
Cuts at: 0 182 1200
Size: 182 1018
DrdI GACnn_nn'nnGTC
Cuts at: 0 964 1200
Size: 964 236
EaeI y'GGCC_r
Cuts at: 0 50 1200
Size: 50 1150
EarI CTCTTCn'nnn_
Cuts at: 0 515 852 1200
Size: 515 337 348
Fragments arranged by size:
515 348 337
EcoICRI GAG'CTC
Cuts at: 0 104 1200
Size: 104 1096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 211 238 292 397 460 1200
Size: 106 105 27 54 105 63 740
Fragments arranged by size:
740 106 105 105 63 54 27
EcoO109I rG'GnC_Cy
Cuts at: 0 945 946 1200
Size: 945 1 254
Fragments arranged by size:
945 254 1
HaeII r_GCGC'y
Cuts at: 0 182 1200
Size: 182 1018
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 578 610 1200
Size: 578 32 590
Fragments arranged by size:
590 578 32
HincII GTy'rAC
Cuts at: 0 282 1200
Size: 282 918
KasI G'GCGC_C
Cuts at: 0 178 1200
Size: 178 1022
MslI CAynn'nnrTG
Cuts at: 0 349 652 1200
Size: 349 303 548
Fragments arranged by size:
548 349 303
MspA1I CmG'CkG
Cuts at: 0 228 270 1200
Size: 228 42 930
Fragments arranged by size:
930 228 42
NarI GG'CG_CC
Cuts at: 0 179 1200
Size: 179 1021
PfoI T'CCnGG_A
Cuts at: 0 106 1200
Size: 106 1094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1200
Size: 433 32 735
Fragments arranged by size:
735 433 32
PspOMI G'GGCC_C
Cuts at: 0 945 1200
Size: 945 255
PstI C_TGCA'G
Cuts at: 0 171 380 401 629 1200
Size: 171 209 21 228 571
Fragments arranged by size:
571 228 209 171 21
PvuII CAG'CTG
Cuts at: 0 270 1200
Size: 270 930
SacI G_AGCT'C
Cuts at: 0 106 1200
Size: 106 1094
SfcI C'TryA_G
Cuts at: 0 167 376 397 548 625 848 1200
Size: 167 209 21 151 77 223 352
Fragments arranged by size:
352 223 209 167 151 77 21
SfoI GGC'GCC
Cuts at: 0 180 1200
Size: 180 1020
StuI AGG'CCT
Cuts at: 0 638 1200
Size: 638 562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 1200
Size: 717 483
XmnI GAAnn'nnTTC
Cuts at: 0 202 1200
Size: 202 998
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaI ApoI
BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I
BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BssSI BstEII BstYI BtgI DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII
KasI MslI MspA1I NarI PfoI PpiI PspOMI PstI
PvuII SacI SfcI SfoI StuI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI ZraI