Restriction map for
pFN21AE4373
(Linear) (Six-base) MAPSORT of: KIEE4373.seq Check: 1876 from: 1 to: 1173
KIEE4373.orf
With 182 enzymes: SgfI *
March 22, 2012 17:51 ..
AccI GT'mk_AC
Cuts at: 0 276 1173
Size: 276 897
Acc65I G'GTAC_C
Cuts at: 0 1067 1173
Size: 1067 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1173
Size: 485 688
ApaI G_GGCC'C
Cuts at: 0 899 1173
Size: 899 274
ApoI r'AATT_y
Cuts at: 0 408 1173
Size: 408 765
BanI G'GyrC_C
Cuts at: 0 1067 1173
Size: 1067 106
BanII G_rGCy'C
Cuts at: 0 543 899 1173
Size: 543 356 274
Fragments arranged by size:
543 356 274
BglII A'GATC_T
Cuts at: 0 490 1173
Size: 490 683
Bme1580I G_kGCm'C
Cuts at: 0 899 1173
Size: 899 274
BmgBI CAC'GTC
Cuts at: 0 280 1173
Size: 280 893
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 1173
Size: 436 737
Bpu10I CC'TnA_GC
Cuts at: 0 185 1173
Size: 185 988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 990 1173
Size: 966 24 183
Fragments arranged by size:
966 183 24
BsaAI yAC'GTr
Cuts at: 0 553 754 874 949 1081 1173
Size: 553 201 120 75 132 92
Fragments arranged by size:
553 201 132 120 92 75
BsaBI GATnn'nnATC
Cuts at: 0 761 1173
Size: 761 412
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 353 470 1064 1173
Size: 353 117 594 109
Fragments arranged by size:
594 353 117 109
BseYI C'CCAG_C
Cuts at: 0 174 1173
Size: 174 999
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1173
Size: 479 694
BsiHKAI G_wGCw'C
Cuts at: 0 543 882 1113 1173
Size: 543 339 231 60
Fragments arranged by size:
543 339 231 60
Bsp1286I G_dGCh'C
Cuts at: 0 543 882 899 1113 1173
Size: 543 339 17 214 60
Fragments arranged by size:
543 339 214 60 17
BssSI C'ACGA_G
Cuts at: 0 683 713 890 1173
Size: 683 30 177 283
Fragments arranged by size:
683 283 177 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 1173
Size: 240 933
BstYI r'GATC_y
Cuts at: 0 490 1173
Size: 490 683
BtgI C'CryG_G
Cuts at: 0 1104 1128 1173
Size: 1104 24 45
Fragments arranged by size:
1104 45 24
BtsI GCAGTG_nn'
Cuts at: 0 528 1173
Size: 528 645
DraIII CAC_nnn'GTG
Cuts at: 0 877 1173
Size: 877 296
EaeI y'GGCC_r
Cuts at: 0 1125 1173
Size: 1125 48
EarI CTCTTCn'nnn_
Cuts at: 0 34 330 531 552 1173
Size: 34 296 201 21 621
Fragments arranged by size:
621 296 201 34 21
EcoICRI GAG'CTC
Cuts at: 0 541 1173
Size: 541 632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1173
Size: 485 688
EcoNI CCTnn'n_nnAGG
Cuts at: 0 317 890 1173
Size: 317 573 283
Fragments arranged by size:
573 317 283
EcoO109I rG'GnC_Cy
Cuts at: 0 310 361 475 895 896 1173
Size: 310 51 114 420 1 277
Fragments arranged by size:
420 310 277 114 51 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 964 988 996 1020 1173
Size: 751 32 181 24 8 24 153
Fragments arranged by size:
751 181 153 32 24 24 8
KpnI G_GTAC'C
Cuts at: 0 1071 1173
Size: 1071 102
MslI CAynn'nnrTG
Cuts at: 0 238 386 827 1173
Size: 238 148 441 346
Fragments arranged by size:
441 346 238 148
MspA1I CmG'CkG
Cuts at: 0 594 1106 1173
Size: 594 512 67
Fragments arranged by size:
594 512 67
NdeI CA'TA_TG
Cuts at: 0 852 1173
Size: 852 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 469 910 1120 1173
Size: 469 441 210 53
Fragments arranged by size:
469 441 210 53
PmlI CAC'GTG
Cuts at: 0 553 754 949 1081 1173
Size: 553 201 195 132 92
Fragments arranged by size:
553 201 195 132 92
PpuMI rG'GwC_Cy
Cuts at: 0 361 475 1173
Size: 361 114 698
Fragments arranged by size:
698 361 114
PspOMI G'GGCC_C
Cuts at: 0 895 1173
Size: 895 278
SacI G_AGCT'C
Cuts at: 0 543 1173
Size: 543 630
SacII CC_GC'GG
Cuts at: 0 1107 1173
Size: 1107 66
SapI GCTCTTCn'nnn_
Cuts at: 0 34 531 1173
Size: 34 497 642
Fragments arranged by size:
642 497 34
ScaI AGT'ACT
Cuts at: 0 110 1173
Size: 110 1063
SfcI C'TryA_G
Cuts at: 0 840 1089 1173
Size: 840 249 84
Fragments arranged by size:
840 249 84
SmlI C'TyrA_G
Cuts at: 0 981 1005 1173
Size: 981 24 168
Fragments arranged by size:
981 168 24
TatI w'GTAC_w
Cuts at: 0 108 1173
Size: 108 1065
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 1173
Size: 86 32 1055
Fragments arranged by size:
1055 86 32
Enzymes that do cut:
AccI Acc65I AcuI ApaI ApoI BanI BanII BglII
Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI BsaBI BseRI
BseYI BsgI BsiHKAI Bsp1286I BssSI BstXI BstYI BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I KpnI MslI MspA1I NdeI PflMI PmlI PpuMI
PspOMI SacI SacII SapI ScaI SfcI SmlI TatI
TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmtI BplI BpmI
BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI