Restriction map for
pFN21AE4371
(Linear) (Six-base) MAPSORT of: KIEE4371.seq Check: 4183 from: 1 to: 1170
KIEE4371.orf
With 182 enzymes: SgfI *
March 22, 2012 17:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 302 1170
Size: 302 868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 577 1170
Size: 131 446 593
Fragments arranged by size:
593 446 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 547 1170
Size: 181 366 623
Fragments arranged by size:
623 366 181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 490 522 1170
Size: 490 32 648
Fragments arranged by size:
648 490 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1099 1170
Size: 1099 71
ApaI G_GGCC'C
Cuts at: 0 13 198 404 1170
Size: 13 185 206 766
Fragments arranged by size:
766 206 185 13
AvaI C'yCGr_G
Cuts at: 0 13 1170
Size: 13 1157
BamHI G'GATC_C
Cuts at: 0 849 1170
Size: 849 321
BanI G'GyrC_C
Cuts at: 0 111 261 864 1170
Size: 111 150 603 306
Fragments arranged by size:
603 306 150 111
BanII G_rGCy'C
Cuts at: 0 13 184 198 404 622 808 1170
Size: 13 171 14 206 218 186 362
Fragments arranged by size:
362 218 206 186 171 14 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 1170
Size: 219 951
BbvCI CC'TCA_GC
Cuts at: 0 1161 1170
Size: 1161 9
BglI GCCn_nnn'nGGC
Cuts at: 0 55 359 1170
Size: 55 304 811
Fragments arranged by size:
811 304 55
Bme1580I G_kGCm'C
Cuts at: 0 13 116 198 266 404 698 1064 1170
Size: 13 103 82 68 138 294 366 106
Fragments arranged by size:
366 294 138 106 103 82 68 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 105 1170
Size: 105 1065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 697 1018 1170
Size: 613 84 321 152
Fragments arranged by size:
613 321 152 84
Bpu10I CC'TnA_GC
Cuts at: 0 634 1161 1170
Size: 634 527 9
Fragments arranged by size:
634 527 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 980 1170
Size: 980 190
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 763 1089 1170
Size: 286 477 326 81
Fragments arranged by size:
477 326 286 81
BseYI C'CCAG_C
Cuts at: 0 197 365 1010 1170
Size: 197 168 645 160
Fragments arranged by size:
645 197 168 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 1170
Size: 4 1166
BsiEI CG_ry'CG
Cuts at: 0 363 1170
Size: 363 807
BsiHKAI G_wGCw'C
Cuts at: 0 808 1170
Size: 808 362
Bsp1286I G_dGCh'C
Cuts at: 0 13 116 184 198 266 404 622 698
Size: 13 103 68 14 68 138 218 76
Cuts at: 698 808 1064 1170
Size: 110 256 106
Fragments arranged by size:
256 218 138 110 106 103 76 68
68 14 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 680 1170
Size: 302 378 490
Fragments arranged by size:
490 378 302
BsrBI CCG'CTC
Cuts at: 0 584 743 1170
Size: 584 159 427
Fragments arranged by size:
584 427 159
BsrFI r'CCGG_y
Cuts at: 0 353 473 551 818 1170
Size: 353 120 78 267 352
Fragments arranged by size:
353 352 267 120 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 1170
Size: 705 465
BstYI r'GATC_y
Cuts at: 0 542 560 849 1170
Size: 542 18 289 321
Fragments arranged by size:
542 321 289 18
Bsu36I CC'TnA_GG
Cuts at: 0 5 107 920 1019 1170
Size: 5 102 813 99 151
Fragments arranged by size:
813 151 102 99 5
BtgI C'CryG_G
Cuts at: 0 153 396 1170
Size: 153 243 774
Fragments arranged by size:
774 243 153
BtsI GCAGTG_nn'
Cuts at: 0 132 1104 1170
Size: 132 972 66
Fragments arranged by size:
972 132 66
DraIII CAC_nnn'GTG
Cuts at: 0 693 1170
Size: 693 477
EaeI y'GGCC_r
Cuts at: 0 26 360 825 1101 1170
Size: 26 334 465 276 69
Fragments arranged by size:
465 334 276 69 26
EagI C'GGCC_G
Cuts at: 0 360 1170
Size: 360 810
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 340 1170
Size: 340 830
EcoICRI GAG'CTC
Cuts at: 0 806 1170
Size: 806 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 577 613 697 1018 1170
Size: 131 446 36 84 321 152
Fragments arranged by size:
446 321 152 131 84 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1036 1170
Size: 1036 134
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 194 369 401 489 597 957
Size: 9 1 184 175 32 88 108 360
Cuts at: 957 1041 1157 1170
Size: 84 116 13
Fragments arranged by size:
360 184 175 116 108 88 84 32
13 9 1
HaeII r_GCGC'y
Cuts at: 0 61 1170
Size: 61 1109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1170
Size: 1123 32 15
Fragments arranged by size:
1123 32 15
MscI TGG'CCA
Cuts at: 0 28 1103 1170
Size: 28 1075 67
Fragments arranged by size:
1075 67 28
MspA1I CmG'CkG
Cuts at: 0 155 258 365 398 912 1014 1170
Size: 155 103 107 33 514 102 156
Fragments arranged by size:
514 156 155 107 103 102 33
NspI r_CATG'y
Cuts at: 0 243 795 1170
Size: 243 552 375
Fragments arranged by size:
552 375 243
PfoI T'CCnGG_A
Cuts at: 0 494 590 852 964 1170
Size: 494 96 262 112 206
Fragments arranged by size:
494 262 206 112 96
PpuMI rG'GwC_Cy
Cuts at: 0 369 1041 1157 1170
Size: 369 672 116 13
Fragments arranged by size:
672 369 116 13
PspOMI G'GGCC_C
Cuts at: 0 9 194 400 1170
Size: 9 185 206 770
Fragments arranged by size:
770 206 185 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 412 444 1170
Size: 412 32 726
Fragments arranged by size:
726 412 32
PstI C_TGCA'G
Cuts at: 0 55 605 662 815 1111 1170
Size: 55 550 57 153 296 59
Fragments arranged by size:
550 296 153 59 57 55
PvuII CAG'CTG
Cuts at: 0 258 912 1170
Size: 258 654 258
Fragments arranged by size:
654 258 258
SacI G_AGCT'C
Cuts at: 0 808 1170
Size: 808 362
SacII CC_GC'GG
Cuts at: 0 156 399 1170
Size: 156 243 771
Fragments arranged by size:
771 243 156
SbfI CC_TGCA'GG
Cuts at: 0 55 815 1170
Size: 55 760 355
Fragments arranged by size:
760 355 55
SfcI C'TryA_G
Cuts at: 0 51 601 658 811 1107 1170
Size: 51 550 57 153 296 63
Fragments arranged by size:
550 296 153 63 57 51
SphI G_CATG'C
Cuts at: 0 795 1170
Size: 795 375
StuI AGG'CCT
Cuts at: 0 49 466 1170
Size: 49 417 704
Fragments arranged by size:
704 417 49
StyI C'CwwG_G
Cuts at: 0 372 698 899 1142 1170
Size: 372 326 201 243 28
Fragments arranged by size:
372 326 243 201 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 576 882 1170
Size: 576 306 288
Fragments arranged by size:
576 306 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 850 1170
Size: 850 320
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BbvCI BglI Bme1580I BplI BpmI
Bpu10I BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MscI MspA1I NspI PfoI PpuMI PspOMI
PsrI PstI PvuII SacI SacII SbfI SfcI SphI
StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EarI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI