Restriction map for
pFN21AE4368
(Linear) (Six-base) MAPSORT of: KIEE4368.seq Check: 2819 from: 1 to: 1158
KIEE4368.orf
With 182 enzymes: SgfI *
March 22, 2012 17:50 ..
AccI GT'mk_AC
Cuts at: 0 611 1158
Size: 611 547
AflII C'TTAA_G
Cuts at: 0 907 1158
Size: 907 251
AflIII A'CryG_T
Cuts at: 0 800 1158
Size: 800 358
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 677 709 992 1024 1064 1096 1158
Size: 677 32 283 32 40 32 62
Fragments arranged by size:
677 283 62 40 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 973 1158
Size: 973 185
ApoI r'AATT_y
Cuts at: 0 277 1158
Size: 277 881
AvrII C'CTAG_G
Cuts at: 0 852 1158
Size: 852 306
BamHI G'GATC_C
Cuts at: 0 992 1158
Size: 992 166
BanI G'GyrC_C
Cuts at: 0 71 1158
Size: 71 1087
BanII G_rGCy'C
Cuts at: 0 131 1158
Size: 131 1027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 11 45 1158
Size: 11 34 1113
Fragments arranged by size:
1113 34 11
Bme1580I G_kGCm'C
Cuts at: 0 74 1158
Size: 74 1084
BmrI ACTGGGnnnn_n'
Cuts at: 0 786 1158
Size: 786 372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 648 1158
Size: 592 56 510
Fragments arranged by size:
592 510 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1090 1091 1158
Size: 26 1064 1 67
Fragments arranged by size:
1064 67 26 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1158
Size: 328 830
BsaI GGTCTCn'nnnn_
Cuts at: 0 636 1158
Size: 636 522
BsaBI GATnn'nnATC
Cuts at: 0 754 1158
Size: 754 404
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1064 1094 1158
Size: 1064 30 64
Fragments arranged by size:
1064 64 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 1158
Size: 194 964
BseYI C'CCAG_C
Cuts at: 0 765 1158
Size: 765 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1158
Size: 51 1107
Bsp1286I G_dGCh'C
Cuts at: 0 74 131 1158
Size: 74 57 1027
Fragments arranged by size:
1027 74 57
BspHI T'CATG_A
Cuts at: 0 321 1121 1158
Size: 321 800 37
Fragments arranged by size:
800 321 37
BsrDI GCAATG_nn'
Cuts at: 0 770 1158
Size: 770 388
BsrFI r'CCGG_y
Cuts at: 0 374 1158
Size: 374 784
BstXI CCAn_nnnn'nTGG
Cuts at: 0 82 1158
Size: 82 1076
BstYI r'GATC_y
Cuts at: 0 488 992 1158
Size: 488 504 166
Fragments arranged by size:
504 488 166
Bsu36I CC'TnA_GG
Cuts at: 0 206 1158
Size: 206 952
BtgI C'CryG_G
Cuts at: 0 1089 1158
Size: 1089 69
BtsI GCAGTG_nn'
Cuts at: 0 1146 1158
Size: 1146 12
EaeI y'GGCC_r
Cuts at: 0 950 1158
Size: 950 208
EarI CTCTTCn'nnn_
Cuts at: 0 87 604 646 677 1139 1158
Size: 87 517 42 31 462 19
Fragments arranged by size:
517 462 87 42 31 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1090 1091 1158
Size: 26 1064 1 67
Fragments arranged by size:
1064 67 26 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 564 1158
Size: 564 594
MscI TGG'CCA
Cuts at: 0 952 1158
Size: 952 206
MslI CAynn'nnrTG
Cuts at: 0 760 1088 1158
Size: 760 328 70
Fragments arranged by size:
760 328 70
MspA1I CmG'CkG
Cuts at: 0 1047 1158
Size: 1047 111
NcoI C'CATG_G
Cuts at: 0 1089 1158
Size: 1089 69
NspI r_CATG'y
Cuts at: 0 234 804 1158
Size: 234 570 354
Fragments arranged by size:
570 354 234
PciI A'CATG_T
Cuts at: 0 800 1158
Size: 800 358
PfoI T'CCnGG_A
Cuts at: 0 1106 1158
Size: 1106 52
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1064 1096 1158
Size: 1064 32 62
Fragments arranged by size:
1064 62 32
PstI C_TGCA'G
Cuts at: 0 1141 1158
Size: 1141 17
PvuII CAG'CTG
Cuts at: 0 1047 1158
Size: 1047 111
SapI GCTCTTCn'nnn_
Cuts at: 0 604 646 677 1158
Size: 604 42 31 481
Fragments arranged by size:
604 481 42 31
SfcI C'TryA_G
Cuts at: 0 1137 1158
Size: 1137 21
SmlI C'TyrA_G
Cuts at: 0 343 907 1158
Size: 343 564 251
Fragments arranged by size:
564 343 251
StuI AGG'CCT
Cuts at: 0 857 1158
Size: 857 301
StyI C'CwwG_G
Cuts at: 0 114 697 852 1089 1158
Size: 114 583 155 237 69
Fragments arranged by size:
583 237 155 114 69
TatI w'GTAC_w
Cuts at: 0 327 1158
Size: 327 831
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 1158
Size: 289 32 837
Fragments arranged by size:
837 289 32
Enzymes that do cut:
AccI AflII AflIII AloI AlwNI ApoI AvrII BamHI
BanI BanII BcgI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaBI BsaXI BseRI BseYI BsgI Bsp1286I BspHI
BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI Eco57MI FalI MscI MslI MspA1I NcoI NspI
PciI PfoI PpiI PstI PvuII SapI SfcI SmlI
StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI