Restriction map for
pFN21AE4366
(Linear) (Six-base) MAPSORT of: KIEE4366.seq Check: 4996 from: 1 to: 1149
KIEE4366.orf
With 182 enzymes: SgfI *
March 22, 2012 17:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1042 1149
Size: 289 753 107
Fragments arranged by size:
753 289 107
AflIII A'CryG_T
Cuts at: 0 728 1149
Size: 728 421
AlwNI CAG_nnn'CTG
Cuts at: 0 516 1020 1101 1149
Size: 516 504 81 48
Fragments arranged by size:
516 504 81 48
ApaI G_GGCC'C
Cuts at: 0 1059 1149
Size: 1059 90
ApoI r'AATT_y
Cuts at: 0 478 845 1149
Size: 478 367 304
Fragments arranged by size:
478 367 304
BanI G'GyrC_C
Cuts at: 0 1042 1149
Size: 1042 107
BanII G_rGCy'C
Cuts at: 0 747 828 1059 1084 1149
Size: 747 81 231 25 65
Fragments arranged by size:
747 231 81 65 25
BbeI G_GCGC'C
Cuts at: 0 1046 1149
Size: 1046 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 1149
Size: 277 872
BciVI GTATCCnnnnn_n'
Cuts at: 0 973 1149
Size: 973 176
BglII A'GATC_T
Cuts at: 0 893 1149
Size: 893 256
BlpI GC'TnA_GC
Cuts at: 0 520 1149
Size: 520 629
Bme1580I G_kGCm'C
Cuts at: 0 1059 1149
Size: 1059 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 1149
Size: 90 1059
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 812 1149
Size: 812 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1149
Size: 31 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1149
Size: 232 917
BsaI GGTCTCn'nnnn_
Cuts at: 0 311 1149
Size: 311 838
BsaBI GATnn'nnATC
Cuts at: 0 769 1149
Size: 769 380
BsaHI Gr'CG_yC
Cuts at: 0 13 1043 1149
Size: 13 1030 106
Fragments arranged by size:
1030 106 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 905 935 1149
Size: 905 30 214
Fragments arranged by size:
905 214 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 801 1149
Size: 801 348
BseYI C'CCAG_C
Cuts at: 0 494 738 1149
Size: 494 244 411
Fragments arranged by size:
494 411 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1149
Size: 1033 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 20 1149
Size: 20 1129
Bsp1286I G_dGCh'C
Cuts at: 0 747 828 1059 1084 1149
Size: 747 81 231 25 65
Fragments arranged by size:
747 231 81 65 25
BspHI T'CATG_A
Cuts at: 0 608 1149
Size: 608 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 933 1149
Size: 933 216
BsrDI GCAATG_nn'
Cuts at: 0 569 1149
Size: 569 580
BsrFI r'CCGG_y
Cuts at: 0 854 1149
Size: 854 295
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 535 1141 1149
Size: 516 19 606 8
Fragments arranged by size:
606 516 19 8
BstEII G'GTnAC_C
Cuts at: 0 546 1149
Size: 546 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 759 1149
Size: 759 390
BstYI r'GATC_y
Cuts at: 0 893 1098 1149
Size: 893 205 51
Fragments arranged by size:
893 205 51
BtgI C'CryG_G
Cuts at: 0 387 909 1046 1149
Size: 387 522 137 103
Fragments arranged by size:
522 387 137 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 125 1149
Size: 125 1024
DrdI GACnn_nn'nnGTC
Cuts at: 0 279 1149
Size: 279 870
EaeI y'GGCC_r
Cuts at: 0 936 1092 1149
Size: 936 156 57
Fragments arranged by size:
936 156 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 289 1042 1149
Size: 31 258 753 107
Fragments arranged by size:
753 258 107 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 460 783 1149
Size: 460 323 366
Fragments arranged by size:
460 366 323
EcoO109I rG'GnC_Cy
Cuts at: 0 875 945 1056 1149
Size: 875 70 111 93
Fragments arranged by size:
875 111 93 70
EcoRI G'AATT_C
Cuts at: 0 845 1149
Size: 845 304
EcoRV GAT'ATC
Cuts at: 0 581 1149
Size: 581 568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 1149
Size: 294 855
FspI TGC'GCA
Cuts at: 0 382 1149
Size: 382 767
HaeII r_GCGC'y
Cuts at: 0 1046 1149
Size: 1046 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 815 847 905 937 1149
Size: 815 32 58 32 212
Fragments arranged by size:
815 212 58 32 32
KasI G'GCGC_C
Cuts at: 0 1042 1149
Size: 1042 107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 356 801 1149
Size: 267 89 445 348
Fragments arranged by size:
445 348 267 89
MspA1I CmG'CkG
Cuts at: 0 89 911 1035 1149
Size: 89 822 124 114
Fragments arranged by size:
822 124 114 89
NarI GG'CG_CC
Cuts at: 0 1043 1149
Size: 1043 106
NcoI C'CATG_G
Cuts at: 0 387 1046 1149
Size: 387 659 103
Fragments arranged by size:
659 387 103
NspI r_CATG'y
Cuts at: 0 237 732 1149
Size: 237 495 417
Fragments arranged by size:
495 417 237
PciI A'CATG_T
Cuts at: 0 728 1149
Size: 728 421
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 1052 1149
Size: 591 461 97
Fragments arranged by size:
591 461 97
PpuMI rG'GwC_Cy
Cuts at: 0 875 945 1149
Size: 875 70 204
Fragments arranged by size:
875 204 70
PsiI TTA'TAA
Cuts at: 0 484 1149
Size: 484 665
PspOMI G'GGCC_C
Cuts at: 0 1055 1149
Size: 1055 94
PvuII CAG'CTG
Cuts at: 0 89 1035 1149
Size: 89 946 114
Fragments arranged by size:
946 114 89
SacII CC_GC'GG
Cuts at: 0 912 1149
Size: 912 237
SanDI GG'GwC_CC
Cuts at: 0 875 1149
Size: 875 274
SfcI C'TryA_G
Cuts at: 0 165 1149
Size: 165 984
SfoI GGC'GCC
Cuts at: 0 1044 1149
Size: 1044 105
SmlI C'TyrA_G
Cuts at: 0 247 1149
Size: 247 902
SphI G_CATG'C
Cuts at: 0 237 1149
Size: 237 912
StyI C'CwwG_G
Cuts at: 0 387 863 959 1046 1149
Size: 387 476 96 87 103
Fragments arranged by size:
476 387 103 96 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 400 1149
Size: 352 48 749
Fragments arranged by size:
749 352 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 394 1149
Size: 394 755
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI BanI BanII BbeI
BbsI BciVI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII
BstXI BstYI BtgI BtgZI DrdI EaeI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I KasI
MmeI MspA1I NarI NcoI NspI PciI PflMI PpuMI
PsiI PspOMI PvuII SacII SanDI SfcI SfoI SmlI
SphI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI
BglI BmgBI BmtI Bpu10I BsaAI BsaWI BsiEI BsiHKAI
BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI
EarI EciI EcoICRI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PstI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI