Restriction map for
pFN21AE4363
(Linear) (Six-base) MAPSORT of: KIEE4363.seq Check: 3911 from: 1 to: 1143
KIEE4363.orf
With 182 enzymes: SgfI *
March 22, 2012 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 23 1084 1143
Size: 23 1061 59
Fragments arranged by size:
1061 59 23
Acc65I G'GTAC_C
Cuts at: 0 711 1143
Size: 711 432
AfeI AGC'GCT
Cuts at: 0 754 846 1143
Size: 754 92 297
Fragments arranged by size:
754 297 92
AflIII A'CryG_T
Cuts at: 0 183 1143
Size: 183 960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 159 358 427 1143
Size: 159 199 69 716
Fragments arranged by size:
716 199 159 69
AlwNI CAG_nnn'CTG
Cuts at: 0 27 64 744 1038 1058 1143
Size: 27 37 680 294 20 85
Fragments arranged by size:
680 294 85 37 27 20
ApaI G_GGCC'C
Cuts at: 0 106 552 1143
Size: 106 446 591
Fragments arranged by size:
591 446 106
AvaI C'yCGr_G
Cuts at: 0 544 1087 1143
Size: 544 543 56
Fragments arranged by size:
544 543 56
AvrII C'CTAG_G
Cuts at: 0 153 1143
Size: 153 990
BamHI G'GATC_C
Cuts at: 0 650 1143
Size: 650 493
BanI G'GyrC_C
Cuts at: 0 116 711 1047 1143
Size: 116 595 336 96
Fragments arranged by size:
595 336 116 96
BanII G_rGCy'C
Cuts at: 0 106 153 552 639 1143
Size: 106 47 399 87 504
Fragments arranged by size:
504 399 106 87 47
BciVI GTATCCnnnnn_n'
Cuts at: 0 1135 1143
Size: 1135 8
BclI T'GATC_A
Cuts at: 0 641 1143
Size: 641 502
BglI GCCn_nnn'nGGC
Cuts at: 0 253 556 1143
Size: 253 303 587
Fragments arranged by size:
587 303 253
BlpI GC'TnA_GC
Cuts at: 0 749 1143
Size: 749 394
Bme1580I G_kGCm'C
Cuts at: 0 99 106 552 1052 1143
Size: 99 7 446 500 91
Fragments arranged by size:
500 446 99 91 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 1143
Size: 324 819
BmtI G_CTAG'C
Cuts at: 0 682 1143
Size: 682 461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 774 1143
Size: 774 369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 307 538 949 1102 1143
Size: 148 159 231 411 153 41
Fragments arranged by size:
411 231 159 153 148 41
BsaWI w'CCGG_w
Cuts at: 0 824 1143
Size: 824 319
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 1143
Size: 280 30 833
Fragments arranged by size:
833 280 30
BseYI C'CCAG_C
Cuts at: 0 783 800 1031 1058 1143
Size: 783 17 231 27 85
Fragments arranged by size:
783 231 85 27 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 1143
Size: 165 978
BsiHKAI G_wGCw'C
Cuts at: 0 832 1143
Size: 832 311
Bsp1286I G_dGCh'C
Cuts at: 0 99 106 153 552 639 832 1052 1143
Size: 99 7 47 399 87 193 220 91
Fragments arranged by size:
399 220 193 99 91 87 47 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 1084 1143
Size: 23 1061 59
Fragments arranged by size:
1061 59 23
BsrDI GCAATG_nn'
Cuts at: 0 136 159 965 1143
Size: 136 23 806 178
Fragments arranged by size:
806 178 136 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 138 1143
Size: 27 111 1005
Fragments arranged by size:
1005 111 27
BstYI r'GATC_y
Cuts at: 0 582 650 1143
Size: 582 68 493
Fragments arranged by size:
582 493 68
BtgI C'CryG_G
Cuts at: 0 833 1143
Size: 833 310
BtsI GCAGTG_nn'
Cuts at: 0 28 62 148 695 1143
Size: 28 34 86 547 448
Fragments arranged by size:
547 448 86 34 28
DrdI GACnn_nn'nnGTC
Cuts at: 0 526 1143
Size: 526 617
EaeI y'GGCC_r
Cuts at: 0 245 1143
Size: 245 898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 307 538 949 1102 1143
Size: 148 159 231 411 153 41
Fragments arranged by size:
411 231 159 153 148 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 1143
Size: 289 854
EcoO109I rG'GnC_Cy
Cuts at: 0 102 103 495 548 549 1083 1143
Size: 102 1 392 53 1 534 60
Fragments arranged by size:
534 392 102 60 53 1 1
HaeII r_GCGC'y
Cuts at: 0 756 848 1143
Size: 756 92 295
Fragments arranged by size:
756 295 92
KpnI G_GTAC'C
Cuts at: 0 715 1143
Size: 715 428
MscI TGG'CCA
Cuts at: 0 247 1143
Size: 247 896
MslI CAynn'nnrTG
Cuts at: 0 141 409 910 1143
Size: 141 268 501 233
Fragments arranged by size:
501 268 233 141
MspA1I CmG'CkG
Cuts at: 0 804 1035 1143
Size: 804 231 108
Fragments arranged by size:
804 231 108
NcoI C'CATG_G
Cuts at: 0 833 1143
Size: 833 310
NheI G'CTAG_C
Cuts at: 0 678 1143
Size: 678 465
NspI r_CATG'y
Cuts at: 0 187 795 909 1143
Size: 187 608 114 234
Fragments arranged by size:
608 234 187 114
PasI CC'CwG_GG
Cuts at: 0 99 1052 1143
Size: 99 953 91
Fragments arranged by size:
953 99 91
PciI A'CATG_T
Cuts at: 0 183 1143
Size: 183 960
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 744 1058 1143
Size: 126 618 314 85
Fragments arranged by size:
618 314 126 85
PfoI T'CCnGG_A
Cuts at: 0 263 284 1143
Size: 263 21 859
Fragments arranged by size:
859 263 21
PpuMI rG'GwC_Cy
Cuts at: 0 495 1143
Size: 495 648
PspOMI G'GGCC_C
Cuts at: 0 102 548 1143
Size: 102 446 595
Fragments arranged by size:
595 446 102
PstI C_TGCA'G
Cuts at: 0 23 634 1143
Size: 23 611 509
Fragments arranged by size:
611 509 23
PvuII CAG'CTG
Cuts at: 0 804 1035 1143
Size: 804 231 108
Fragments arranged by size:
804 231 108
SanDI GG'GwC_CC
Cuts at: 0 495 1143
Size: 495 648
SfcI C'TryA_G
Cuts at: 0 19 166 630 1143
Size: 19 147 464 513
Fragments arranged by size:
513 464 147 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 253 1143
Size: 253 890
SmaI CCC'GGG
Cuts at: 0 546 1143
Size: 546 597
SphI G_CATG'C
Cuts at: 0 795 1143
Size: 795 348
StuI AGG'CCT
Cuts at: 0 564 970 1143
Size: 564 406 173
Fragments arranged by size:
564 406 173
StyI C'CwwG_G
Cuts at: 0 86 153 248 668 833 853 1143
Size: 86 67 95 420 165 20 290
Fragments arranged by size:
420 290 165 95 86 67 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 934 1143
Size: 934 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 1143
Size: 280 32 831
Fragments arranged by size:
831 280 32
Tth111I GACn'n_nGTC
Cuts at: 0 223 1104 1143
Size: 223 881 39
Fragments arranged by size:
881 223 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 562 973 1143
Size: 84 478 411 170
Fragments arranged by size:
478 411 170 84
XmaI C'CCGG_G
Cuts at: 0 544 1143
Size: 544 599
Enzymes that do cut:
AarI Acc65I AfeI AflIII AlfI AlwNI ApaI AvaI
AvrII BamHI BanI BanII BciVI BclI BglI BlpI
Bme1580I BmrI BmtI BplI BpmI BsaWI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI
BtsI DrdI EaeI Eco57MI EcoNI EcoO109I HaeII KpnI
MscI MslI MspA1I NcoI NheI NspI PasI PciI
PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SfcI
SfiI SmaI SphI StuI StyI TaqII TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BbeI
BbsI BbvCI BcgI BfrBI BglII BmgBI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI SwaI TatI XbaI XhoI
XmnI ZraI