Restriction map for
pFN21AE4361
(Linear) (Six-base) MAPSORT of: KIEE4361.seq Check: 6455 from: 1 to: 1140
KIEE4361.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AatII G_ACGT'C
Cuts at: 0 378 1112 1140
Size: 378 734 28
Fragments arranged by size:
734 378 28
AccI GT'mk_AC
Cuts at: 0 1097 1112 1140
Size: 1097 15 28
Fragments arranged by size:
1097 28 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1140
Size: 115 1025
AlwNI CAG_nnn'CTG
Cuts at: 0 472 887 1140
Size: 472 415 253
Fragments arranged by size:
472 415 253
ApaI G_GGCC'C
Cuts at: 0 466 1140
Size: 466 674
ApoI r'AATT_y
Cuts at: 0 52 1140
Size: 52 1088
AvaI C'yCGr_G
Cuts at: 0 295 1140
Size: 295 845
AvrII C'CTAG_G
Cuts at: 0 772 1140
Size: 772 368
BanI G'GyrC_C
Cuts at: 0 523 1140
Size: 523 617
BanII G_rGCy'C
Cuts at: 0 466 771 944 1039 1140
Size: 466 305 173 95 101
Fragments arranged by size:
466 305 173 101 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 404 812 1140
Size: 404 408 328
Fragments arranged by size:
408 404 328
BbvCI CC'TCA_GC
Cuts at: 0 534 1140
Size: 534 606
BglI GCCn_nnn'nGGC
Cuts at: 0 232 1140
Size: 232 908
BlpI GC'TnA_GC
Cuts at: 0 938 1140
Size: 938 202
Bme1580I G_kGCm'C
Cuts at: 0 466 528 1140
Size: 466 62 612
Fragments arranged by size:
612 466 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 510 1140
Size: 510 630
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 407 1140
Size: 407 733
Bpu10I CC'TnA_GC
Cuts at: 0 534 1140
Size: 534 606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 295 1140
Size: 49 246 845
Fragments arranged by size:
845 246 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 587 611 671 707 713 1140
Size: 232 355 24 60 36 6 427
Fragments arranged by size:
427 355 232 60 36 24 6
BsaBI GATnn'nnATC
Cuts at: 0 659 665 671 1140
Size: 659 6 6 469
Fragments arranged by size:
659 469 6 6
BsaHI Gr'CG_yC
Cuts at: 0 375 1109 1140
Size: 375 734 31
Fragments arranged by size:
734 375 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 348 522 1140
Size: 49 299 174 618
Fragments arranged by size:
618 299 174 49
BsiHKAI G_wGCw'C
Cuts at: 0 944 1140
Size: 944 196
Bsp1286I G_dGCh'C
Cuts at: 0 466 528 771 944 1039 1140
Size: 466 62 243 173 95 101
Fragments arranged by size:
466 243 173 101 95 62
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 365 731 1140
Size: 365 366 409
Fragments arranged by size:
409 366 365
BsrBI CCG'CTC
Cuts at: 0 609 645 705 1140
Size: 609 36 60 435
Fragments arranged by size:
609 435 60 36
BsrDI GCAATG_nn'
Cuts at: 0 82 1140
Size: 82 1058
BsrGI T'GTAC_A
Cuts at: 0 966 1140
Size: 966 174
Bsu36I CC'TnA_GG
Cuts at: 0 1061 1140
Size: 1061 79
BtgI C'CryG_G
Cuts at: 0 828 869 1140
Size: 828 41 271
Fragments arranged by size:
828 271 41
BtsI GCAGTG_nn'
Cuts at: 0 525 1140
Size: 525 615
EarI CTCTTCn'nnn_
Cuts at: 0 26 446 1140
Size: 26 420 694
Fragments arranged by size:
694 420 26
EcoICRI GAG'CTC
Cuts at: 0 942 1140
Size: 942 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1140
Size: 115 1025
EcoNI CCTnn'n_nnAGG
Cuts at: 0 776 1140
Size: 776 364
EcoO109I rG'GnC_Cy
Cuts at: 0 291 462 730 833 950 1085 1140
Size: 291 171 268 103 117 135 55
Fragments arranged by size:
291 268 171 135 117 103 55
HincII GTy'rAC
Cuts at: 0 981 1140
Size: 981 159
HindIII A'AGCT_T
Cuts at: 0 492 1140
Size: 492 648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1140
Size: 725 415
MspA1I CmG'CkG
Cuts at: 0 201 1140
Size: 201 939
NcoI C'CATG_G
Cuts at: 0 869 1140
Size: 869 271
PasI CC'CwG_GG
Cuts at: 0 466 1140
Size: 466 674
PflMI CCAn_nnn'nTGG
Cuts at: 0 472 1140
Size: 472 668
PpuMI rG'GwC_Cy
Cuts at: 0 291 730 950 1085 1140
Size: 291 439 220 135 55
Fragments arranged by size:
439 291 220 135 55
PspOMI G'GGCC_C
Cuts at: 0 462 1140
Size: 462 678
PstI C_TGCA'G
Cuts at: 0 47 1140
Size: 47 1093
PvuII CAG'CTG
Cuts at: 0 201 1140
Size: 201 939
SacI G_AGCT'C
Cuts at: 0 944 1140
Size: 944 196
SanDI GG'GwC_CC
Cuts at: 0 950 1140
Size: 950 190
SfcI C'TryA_G
Cuts at: 0 43 1098 1140
Size: 43 1055 42
Fragments arranged by size:
1055 43 42
SmlI C'TyrA_G
Cuts at: 0 64 274 1140
Size: 64 210 866
Fragments arranged by size:
866 210 64
StyI C'CwwG_G
Cuts at: 0 126 772 869 1140
Size: 126 646 97 271
Fragments arranged by size:
646 271 126 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 611 731 737 831 894 1140
Size: 611 120 6 94 63 246
Fragments arranged by size:
611 246 120 94 63 6
TatI w'GTAC_w
Cuts at: 0 966 1140
Size: 966 174
XmnI GAAnn'nnTTC
Cuts at: 0 52 1140
Size: 52 1088
ZraI GAC'GTC
Cuts at: 0 376 1110 1140
Size: 376 734 30
Fragments arranged by size:
734 376 30
Enzymes that do cut:
AatII AccI AcuI AlwNI ApaI ApoI AvaI AvrII
BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BseRI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrGI Bsu36I BtgI BtsI
EarI EcoICRI Eco57MI EcoNI EcoO109I HincII HindIII MmeI
MspA1I NcoI PasI PflMI PpuMI PspOMI PstI PvuII
SacI SanDI SfcI SmlI StyI TaqII TatI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BpmI BsaAI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI