Restriction map for
pFN21AE4360
(Linear) (Six-base) MAPSORT of: KIEE4360.seq Check: 3485 from: 1 to: 1137
KIEE4360.orf
With 182 enzymes: SgfI *
March 22, 2012 17:49 ..
AatII G_ACGT'C
Cuts at: 0 877 1137
Size: 877 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1137
Size: 205 932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 329 598 1137
Size: 329 269 539
Fragments arranged by size:
539 329 269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 680 712 1137
Size: 680 32 425
Fragments arranged by size:
680 425 32
AlwNI CAG_nnn'CTG
Cuts at: 0 183 759 925 1137
Size: 183 576 166 212
Fragments arranged by size:
576 212 183 166
ApoI r'AATT_y
Cuts at: 0 1000 1137
Size: 1000 137
AvaI C'yCGr_G
Cuts at: 0 365 1137
Size: 365 772
BanI G'GyrC_C
Cuts at: 0 484 523 1137
Size: 484 39 614
Fragments arranged by size:
614 484 39
BanII G_rGCy'C
Cuts at: 0 176 647 909 1137
Size: 176 471 262 228
Fragments arranged by size:
471 262 228 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 1137
Size: 193 944
BbvCI CC'TCA_GC
Cuts at: 0 23 1137
Size: 23 1114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 1040 1137
Size: 1006 34 97
Fragments arranged by size:
1006 97 34
BclI T'GATC_A
Cuts at: 0 336 1137
Size: 336 801
BfrBI ATG'CAT
Cuts at: 0 457 1137
Size: 457 680
BglI GCCn_nnn'nGGC
Cuts at: 0 774 1137
Size: 774 363
BglII A'GATC_T
Cuts at: 0 506 1137
Size: 506 631
BlpI GC'TnA_GC
Cuts at: 0 809 1137
Size: 809 328
Bme1580I G_kGCm'C
Cuts at: 0 197 489 1080 1137
Size: 197 292 591 57
Fragments arranged by size:
591 292 197 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 314 326 483 945 1137
Size: 255 59 12 157 462 192
Fragments arranged by size:
462 255 192 157 59 12
Bpu10I CC'TnA_GC
Cuts at: 0 23 794 1028 1052 1137
Size: 23 771 234 24 85
Fragments arranged by size:
771 234 85 24 23
BsaHI Gr'CG_yC
Cuts at: 0 40 53 874 1137
Size: 40 13 821 263
Fragments arranged by size:
821 263 40 13
BsaWI w'CCGG_w
Cuts at: 0 995 1137
Size: 995 142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 490 619 1137
Size: 383 107 129 518
Fragments arranged by size:
518 383 129 107
BseYI C'CCAG_C
Cuts at: 0 176 297 480 596 925 1137
Size: 176 121 183 116 329 212
Fragments arranged by size:
329 212 183 176 121 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 937 1137
Size: 523 414 200
Fragments arranged by size:
523 414 200
BsiHKAI G_wGCw'C
Cuts at: 0 713 1137
Size: 713 424
BsmBI CGTCTCn'nnnn_
Cuts at: 0 865 1137
Size: 865 272
Bsp1286I G_dGCh'C
Cuts at: 0 176 197 489 647 713 909 1080 1137
Size: 176 21 292 158 66 196 171 57
Fragments arranged by size:
292 196 176 171 158 66 57 21
BspEI T'CCGG_A
Cuts at: 0 995 1137
Size: 995 142
BspHI T'CATG_A
Cuts at: 0 833 1137
Size: 833 304
BsrBI CCG'CTC
Cuts at: 0 975 1137
Size: 975 162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 361 1137
Size: 361 776
BstYI r'GATC_y
Cuts at: 0 437 506 1137
Size: 437 69 631
Fragments arranged by size:
631 437 69
Bsu36I CC'TnA_GG
Cuts at: 0 1085 1137
Size: 1085 52
BtgI C'CryG_G
Cuts at: 0 890 1137
Size: 890 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1116 1137
Size: 1116 21
BtsI GCAGTG_nn'
Cuts at: 0 15 555 757 1137
Size: 15 540 202 380
Fragments arranged by size:
540 380 202 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 1137
Size: 64 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1137
Size: 205 932
EcoO109I rG'GnC_Cy
Cuts at: 0 328 1041 1137
Size: 328 713 96
Fragments arranged by size:
713 328 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 861 1137
Size: 861 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 811 843 1137
Size: 595 32 184 32 294
Fragments arranged by size:
595 294 184 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1137
Size: 383 754
MspA1I CmG'CkG
Cuts at: 0 108 951 1137
Size: 108 843 186
Fragments arranged by size:
843 186 108
NcoI C'CATG_G
Cuts at: 0 890 1137
Size: 890 247
NsiI A_TGCA'T
Cuts at: 0 459 1137
Size: 459 678
NspI r_CATG'y
Cuts at: 0 781 1137
Size: 781 356
PflMI CCAn_nnn'nTGG
Cuts at: 0 896 1137
Size: 896 241
PfoI T'CCnGG_A
Cuts at: 0 324 1137
Size: 324 813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 1137
Size: 6 32 1099
Fragments arranged by size:
1099 32 6
PpuMI rG'GwC_Cy
Cuts at: 0 328 1041 1137
Size: 328 713 96
Fragments arranged by size:
713 328 96
PspXI vC'TCGA_Gb
Cuts at: 0 365 1137
Size: 365 772
PstI C_TGCA'G
Cuts at: 0 113 308 1065 1137
Size: 113 195 757 72
Fragments arranged by size:
757 195 113 72
PvuII CAG'CTG
Cuts at: 0 951 1137
Size: 951 186
ScaI AGT'ACT
Cuts at: 0 722 1137
Size: 722 415
SfcI C'TryA_G
Cuts at: 0 109 304 1061 1137
Size: 109 195 757 76
Fragments arranged by size:
757 195 109 76
SmlI C'TyrA_G
Cuts at: 0 365 1137
Size: 365 772
StuI AGG'CCT
Cuts at: 0 622 1137
Size: 622 515
StyI C'CwwG_G
Cuts at: 0 496 890 1137
Size: 496 394 247
Fragments arranged by size:
496 394 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 417 472 489 1137
Size: 111 306 55 17 648
Fragments arranged by size:
648 306 111 55 17
TatI w'GTAC_w
Cuts at: 0 659 720 1137
Size: 659 61 417
Fragments arranged by size:
659 417 61
XhoI C'TCGA_G
Cuts at: 0 365 1137
Size: 365 772
ZraI GAC'GTC
Cuts at: 0 875 1137
Size: 875 262
Enzymes that do cut:
AatII AcuI AlfI AloI AlwNI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI Bpu10I BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BstAPI
BstYI Bsu36I BtgI BtgZI BtsI EciI Eco57MI EcoO109I
FalI Hin4I MmeI MspA1I NcoI NsiI NspI PflMI
PfoI PpiI PpuMI PspXI PstI PvuII ScaI SfcI
SmlI StuI StyI TaqII TatI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BmgBI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XcmI XmaI XmnI