Restriction map for
pFN21AE4354
(Linear) (Six-base) MAPSORT of: KIEE4354.seq Check: 3505 from: 1 to: 1113
KIEE4354.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 117 889 1113
Size: 117 772 224
Fragments arranged by size:
772 224 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1113
Size: 977 136
AflIII A'CryG_T
Cuts at: 0 409 1113
Size: 409 704
AleI CACnn'nnGTG
Cuts at: 0 1081 1113
Size: 1081 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 410 1113
Size: 410 703
ApaLI G'TGCA_C
Cuts at: 0 210 1113
Size: 210 903
ApoI r'AATT_y
Cuts at: 0 691 1113
Size: 691 422
AvaI C'yCGr_G
Cuts at: 0 817 1113
Size: 817 296
BamHI G'GATC_C
Cuts at: 0 1015 1113
Size: 1015 98
BanI G'GyrC_C
Cuts at: 0 248 1113
Size: 248 865
BanII G_rGCy'C
Cuts at: 0 463 824 932 1113
Size: 463 361 108 181
Fragments arranged by size:
463 361 181 108
Bme1580I G_kGCm'C
Cuts at: 0 214 1113
Size: 214 899
BmgBI CAC'GTC
Cuts at: 0 410 1113
Size: 410 703
BmrI ACTGGGnnnn_n'
Cuts at: 0 350 1113
Size: 350 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 211 1006 1113
Size: 138 73 795 107
Fragments arranged by size:
795 138 107 73
BsaAI yAC'GTr
Cuts at: 0 372 1104 1113
Size: 372 732 9
Fragments arranged by size:
732 372 9
BsaBI GATnn'nnATC
Cuts at: 0 97 1113
Size: 97 1016
BsaWI w'CCGG_w
Cuts at: 0 455 1113
Size: 455 658
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1113
Size: 17 30 1066
Fragments arranged by size:
1066 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 427 727 940 1009 1113
Size: 86 341 300 213 69 104
Fragments arranged by size:
341 300 213 104 86 69
BseYI C'CCAG_C
Cuts at: 0 42 787 1113
Size: 42 745 326
Fragments arranged by size:
745 326 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 674 923 1113
Size: 145 529 249 190
Fragments arranged by size:
529 249 190 145
BsiHKAI G_wGCw'C
Cuts at: 0 214 1113
Size: 214 899
BsmI GAATG_Cn'
Cuts at: 0 144 1113
Size: 144 969
BsmBI CGTCTCn'nnnn_
Cuts at: 0 266 1113
Size: 266 847
Bsp1286I G_dGCh'C
Cuts at: 0 214 463 824 932 1113
Size: 214 249 361 108 181
Fragments arranged by size:
361 249 214 181 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 508 889 1113
Size: 117 391 381 224
Fragments arranged by size:
391 381 224 117
BsrBI CCG'CTC
Cuts at: 0 1097 1113
Size: 1097 16
BstXI CCAn_nnnn'nTGG
Cuts at: 0 605 978 1113
Size: 605 373 135
Fragments arranged by size:
605 373 135
BstYI r'GATC_y
Cuts at: 0 432 1015 1113
Size: 432 583 98
Fragments arranged by size:
583 432 98
Bsu36I CC'TnA_GG
Cuts at: 0 923 1113
Size: 923 190
BtsI GCAGTG_nn'
Cuts at: 0 404 1113
Size: 404 709
DraIII CAC_nnn'GTG
Cuts at: 0 476 1113
Size: 476 637
EaeI y'GGCC_r
Cuts at: 0 713 947 1113
Size: 713 234 166
Fragments arranged by size:
713 234 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 211 977 1006 1113
Size: 138 73 766 29 107
Fragments arranged by size:
766 138 107 73 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 442 1113
Size: 226 216 671
Fragments arranged by size:
671 226 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 466 498 717 749 790 822
Size: 17 32 417 32 219 32 41 32
Cuts at: 822 1113
Size: 291
Fragments arranged by size:
417 291 219 41 32 32 32 32
17
MscI TGG'CCA
Cuts at: 0 715 949 1113
Size: 715 234 164
Fragments arranged by size:
715 234 164
MslI CAynn'nnrTG
Cuts at: 0 392 1081 1113
Size: 392 689 32
Fragments arranged by size:
689 392 32
NspI r_CATG'y
Cuts at: 0 623 1113
Size: 623 490
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 894 1113
Size: 876 18 219
Fragments arranged by size:
876 219 18
PfoI T'CCnGG_A
Cuts at: 0 154 227 848 1113
Size: 154 73 621 265
Fragments arranged by size:
621 265 154 73
PmlI CAC'GTG
Cuts at: 0 372 1113
Size: 372 741
SfcI C'TryA_G
Cuts at: 0 177 1113
Size: 177 936
SmaI CCC'GGG
Cuts at: 0 819 1113
Size: 819 294
StuI AGG'CCT
Cuts at: 0 199 1006 1113
Size: 199 807 107
Fragments arranged by size:
807 199 107
StyI C'CwwG_G
Cuts at: 0 837 1044 1113
Size: 837 207 69
Fragments arranged by size:
837 207 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 883 1006 1113
Size: 883 123 107
Fragments arranged by size:
883 123 107
TatI w'GTAC_w
Cuts at: 0 87 1113
Size: 87 1026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 519 723 1113
Size: 519 204 390
Fragments arranged by size:
519 390 204
XmaI C'CCGG_G
Cuts at: 0 817 1113
Size: 817 296
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI ApaLI ApoI AvaI
BamHI BanI BanII Bme1580I BmgBI BmrI BpmI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BstXI BstYI Bsu36I BtsI
DraIII EaeI Eco57MI EcoNI Hin4I MscI MslI NspI
PflMI PfoI PmlI SfcI SmaI StuI StyI TaqII
TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaHI
BsiEI BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmnI
ZraI