(Linear) (Six-base) MAPSORT of: KIEE4351.seq  Check: 8085  from: 1  to: 1095



KIEE4351.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1017    1095 
   Size:       1017      78
 
AclI AA'CG_TT

Cuts at:      0     683    1095 
   Size:        683     412
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     407     473    1095 
   Size:         95     312      66     622

  Fragments arranged by size: 

                 622     312      95      66
 
AfeI AGC'GCT

Cuts at:      0     752    1095 
   Size:        752     343
 
AlwNI CAG_nnn'CTG

Cuts at:      0     669    1095 
   Size:        669     426
 
ApoI r'AATT_y

Cuts at:      0     124     233     324    1095 
   Size:        124     109      91     771

  Fragments arranged by size: 

                 771     124     109      91
 
BanII G_rGCy'C

Cuts at:      0     658    1095 
   Size:        658     437
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     149     977    1095 
   Size:        149     828     118

  Fragments arranged by size: 

                 828     149     118
 
BclI T'GATC_A

Cuts at:      0     660    1095 
   Size:        660     435
 
BfrBI ATG'CAT

Cuts at:      0     307    1095 
   Size:        307     788
 
BglII A'GATC_T

Cuts at:      0     255    1095 
   Size:        255     840
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1016    1095 
   Size:       1016      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245    1095 
   Size:        245     850
 
Bpu10I CC'TnA_GC

Cuts at:      0    1013    1095 
   Size:       1013      82
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     977    1095 
   Size:        977     118
 
BsiHKAI G_wGCw'C

Cuts at:      0      26    1095 
   Size:         26    1069
 
BsiWI C'GTAC_G

Cuts at:      0     859    1095 
   Size:        859     236
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     658    1095 
   Size:         26     632     437

  Fragments arranged by size: 

                 632     437      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1017    1095 
   Size:       1017      78
 
BsrGI T'GTAC_A

Cuts at:      0     634    1095 
   Size:        634     461
 
BstYI r'GATC_y

Cuts at:      0     255     448    1095 
   Size:        255     193     647

  Fragments arranged by size: 

                 647     255     193
 
BtsI GCAGTG_nn'

Cuts at:      0     866    1095 
   Size:        866     229
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     612    1095 
   Size:         71     541     483

  Fragments arranged by size: 

                 541     483      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     245     407     473    1095 
   Size:         95     150     162      66     622

  Fragments arranged by size: 

                 622     162     150      95      66
 
EcoRV GAT'ATC

Cuts at:      0     176     828    1095 
   Size:        176     652     267

  Fragments arranged by size: 

                 652     267     176
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     520    1095 
   Size:        520     575
 
HaeII r_GCGC'y

Cuts at:      0       7     754    1095 
   Size:          7     747     341

  Fragments arranged by size: 

                 747     341       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     273     280     305     312    1095 
   Size:        273       7      25       7     783

  Fragments arranged by size: 

                 783     273      25       7       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     947    1021    1095 
   Size:        947      74      74

  Fragments arranged by size: 

                 947      74      74
 
MslI CAynn'nnrTG

Cuts at:      0     354    1095 
   Size:        354     741
 
MspA1I CmG'CkG

Cuts at:      0     156     184     632    1095 
   Size:        156      28     448     463

  Fragments arranged by size: 

                 463     448     156      28
 
NsiI A_TGCA'T

Cuts at:      0     309    1095 
   Size:        309     786
 
PasI CC'CwG_GG

Cuts at:      0     903    1095 
   Size:        903     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     560    1095 
   Size:        560     535
 
PvuII CAG'CTG

Cuts at:      0     184     632    1095 
   Size:        184     448     463

  Fragments arranged by size: 

                 463     448     184
 
ScaI AGT'ACT

Cuts at:      0     616    1095 
   Size:        616     479
 
SfcI C'TryA_G

Cuts at:      0    1088    1095 
   Size:       1088       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1033    1095 
   Size:       1033      62
 
TatI w'GTAC_w

Cuts at:      0     270     614     634    1095 
   Size:        270     344      20     461

  Fragments arranged by size: 

                 461     344     270      20
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     151     183    1095 
   Size:        151      32     912

  Fragments arranged by size: 

                 912     151      32
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AfeI    AlwNI     ApoI    BanII     BbsI
     BclI    BfrBI    BglII     BplI     BpmI   Bpu10I    BseRI  BsiHKAI
    BsiWI Bsp1286I    BspMI    BsrGI    BstYI     BtsI     EarI  Eco57MI
    EcoRV     FalI    HaeII    Hin4I     MmeI     MslI   MspA1I     NsiI
     PasI    PflMI    PvuII     ScaI     SfcI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI