Restriction map for
pFN21AE4349
(Linear) (Six-base) MAPSORT of: KIEE4349.seq Check: 6808 from: 1 to: 1092
KIEE4349.orf
With 182 enzymes: SgfI *
March 22, 2012 17:47 ..
AccI GT'mk_AC
Cuts at: 0 858 1092
Size: 858 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1092
Size: 791 301
AflII C'TTAA_G
Cuts at: 0 21 1092
Size: 21 1071
AleI CACnn'nnGTG
Cuts at: 0 113 1092
Size: 113 979
AlwNI CAG_nnn'CTG
Cuts at: 0 49 1092
Size: 49 1043
BbsI GAAGACnn'nnnn_
Cuts at: 0 797 1058 1092
Size: 797 261 34
Fragments arranged by size:
797 261 34
BbvCI CC'TCA_GC
Cuts at: 0 695 1092
Size: 695 397
BfrBI ATG'CAT
Cuts at: 0 3 1092
Size: 3 1089
BglI GCCn_nnn'nGGC
Cuts at: 0 176 1092
Size: 176 916
BmgBI CAC'GTC
Cuts at: 0 204 1092
Size: 204 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1092
Size: 914 178
Bpu10I CC'TnA_GC
Cuts at: 0 695 1092
Size: 695 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 378 408 1092
Size: 378 30 684
Fragments arranged by size:
684 378 30
BsiHKAI G_wGCw'C
Cuts at: 0 326 442 1092
Size: 326 116 650
Fragments arranged by size:
650 326 116
BsmI GAATG_Cn'
Cuts at: 0 512 584 1092
Size: 512 72 508
Fragments arranged by size:
512 508 72
Bsp1286I G_dGCh'C
Cuts at: 0 326 442 1092
Size: 326 116 650
Fragments arranged by size:
650 326 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 73 1092
Size: 73 1019
BsrDI GCAATG_nn'
Cuts at: 0 941 1092
Size: 941 151
BstBI TT'CG_AA
Cuts at: 0 432 1092
Size: 432 660
BstXI CCAn_nnnn'nTGG
Cuts at: 0 115 1092
Size: 115 977
BtgI C'CryG_G
Cuts at: 0 327 1085 1092
Size: 327 758 7
Fragments arranged by size:
758 327 7
BtsI GCAGTG_nn'
Cuts at: 0 721 1092
Size: 721 371
EaeI y'GGCC_r
Cuts at: 0 866 1092
Size: 866 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 914 1092
Size: 791 123 178
Fragments arranged by size:
791 178 123
EcoO109I rG'GnC_Cy
Cuts at: 0 243 1092
Size: 243 849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 975 1092
Size: 975 117
HindIII A'AGCT_T
Cuts at: 0 1013 1092
Size: 1013 79
MfeI C'AATT_G
Cuts at: 0 398 1092
Size: 398 694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 475 508 523 541 595 613 1092
Size: 439 36 33 15 18 54 18 479
Fragments arranged by size:
479 439 54 36 33 18 18 15
MslI CAynn'nnrTG
Cuts at: 0 113 130 132 1092
Size: 113 17 2 960
Fragments arranged by size:
960 113 17 2
MspA1I CmG'CkG
Cuts at: 0 286 1092
Size: 286 806
NsiI A_TGCA'T
Cuts at: 0 5 1092
Size: 5 1087
NspI r_CATG'y
Cuts at: 0 170 1092
Size: 170 922
PflMI CCAn_nnn'nTGG
Cuts at: 0 137 1092
Size: 137 955
PpuMI rG'GwC_Cy
Cuts at: 0 243 1092
Size: 243 849
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 842 874 1092
Size: 842 32 218
Fragments arranged by size:
842 218 32
PstI C_TGCA'G
Cuts at: 0 728 1092
Size: 728 364
PvuII CAG'CTG
Cuts at: 0 286 1092
Size: 286 806
SfcI C'TryA_G
Cuts at: 0 50 111 724 1092
Size: 50 61 613 368
Fragments arranged by size:
613 368 61 50
SmlI C'TyrA_G
Cuts at: 0 8 21 1092
Size: 8 13 1071
Fragments arranged by size:
1071 13 8
StyI C'CwwG_G
Cuts at: 0 380 1092
Size: 380 712
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 320 393 922 1092
Size: 320 73 529 170
Fragments arranged by size:
529 320 170 73
TatI w'GTAC_w
Cuts at: 0 764 1092
Size: 764 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 378 396 410 428 432 464 1092
Size: 378 18 14 18 4 32 628
Fragments arranged by size:
628 378 32 18 18 14 4
Tth111I GACn'n_nGTC
Cuts at: 0 801 1092
Size: 801 291
XbaI T'CTAG_A
Cuts at: 0 846 1092
Size: 846 246
Enzymes that do cut:
AccI AcuI AflII AleI AlwNI BbsI BbvCI BfrBI
BglI BmgBI BpmI Bpu10I BsaXI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstBI BstXI BtgI BtsI EaeI Eco57MI
EcoO109I FalI HindIII MfeI MmeI MslI MspA1I NsiI
NspI PflMI PpuMI PsrI PstI PvuII SfcI SmlI
StyI TaqII TatI TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XcmI XhoI XmaI XmnI ZraI