Restriction map for
pFN21AE4345
(Linear) (Six-base) MAPSORT of: KIEE4345.seq Check: 4852 from: 1 to: 1083
KIEE4345.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
Acc65I G'GTAC_C
Cuts at: 0 70 1083
Size: 70 1013
AlwNI CAG_nnn'CTG
Cuts at: 0 615 1083
Size: 615 468
ApaI G_GGCC'C
Cuts at: 0 980 1083
Size: 980 103
ApoI r'AATT_y
Cuts at: 0 382 1083
Size: 382 701
AvaI C'yCGr_G
Cuts at: 0 721 1083
Size: 721 362
BanI G'GyrC_C
Cuts at: 0 70 764 1083
Size: 70 694 319
Fragments arranged by size:
694 319 70
BanII G_rGCy'C
Cuts at: 0 980 1083
Size: 980 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 400 1083
Size: 400 683
BfrBI ATG'CAT
Cuts at: 0 632 1083
Size: 632 451
BlpI GC'TnA_GC
Cuts at: 0 1027 1083
Size: 1027 56
Bme1580I G_kGCm'C
Cuts at: 0 980 1083
Size: 980 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 975 1083
Size: 975 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 502 857 867 1083
Size: 274 228 355 10 216
Fragments arranged by size:
355 274 228 216 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 871 1083
Size: 871 212
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 854 1083
Size: 854 229
BseYI C'CCAG_C
Cuts at: 0 455 908 1083
Size: 455 453 175
Fragments arranged by size:
455 453 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 601 697 1083
Size: 182 419 96 386
Fragments arranged by size:
419 386 182 96
BsmI GAATG_Cn'
Cuts at: 0 634 1083
Size: 634 449
Bsp1286I G_dGCh'C
Cuts at: 0 980 1083
Size: 980 103
BspHI T'CATG_A
Cuts at: 0 357 1083
Size: 357 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 1083
Size: 305 778
BsrDI GCAATG_nn'
Cuts at: 0 21 158 1083
Size: 21 137 925
Fragments arranged by size:
925 137 21
BssSI C'ACGA_G
Cuts at: 0 971 1083
Size: 971 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 615 1083
Size: 615 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 1083
Size: 561 522
BstYI r'GATC_y
Cuts at: 0 611 696 1083
Size: 611 85 387
Fragments arranged by size:
611 387 85
Bsu36I CC'TnA_GG
Cuts at: 0 623 760 926 1083
Size: 623 137 166 157
Fragments arranged by size:
623 166 157 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 726 1083
Size: 726 357
BtsI GCAGTG_nn'
Cuts at: 0 789 953 1083
Size: 789 164 130
Fragments arranged by size:
789 164 130
EaeI y'GGCC_r
Cuts at: 0 1013 1083
Size: 1013 70
EarI CTCTTCn'nnn_
Cuts at: 0 412 441 1083
Size: 412 29 642
Fragments arranged by size:
642 412 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 1083
Size: 46 1037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 502 857 867 1083
Size: 274 228 355 10 216
Fragments arranged by size:
355 274 228 216 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 759 1083
Size: 759 324
EcoO109I rG'GnC_Cy
Cuts at: 0 294 653 752 976 977 1083
Size: 294 359 99 224 1 106
Fragments arranged by size:
359 294 224 106 99 1
KpnI G_GTAC'C
Cuts at: 0 74 1083
Size: 74 1009
MscI TGG'CCA
Cuts at: 0 1015 1083
Size: 1015 68
MslI CAynn'nnrTG
Cuts at: 0 163 917 1083
Size: 163 754 166
Fragments arranged by size:
754 166 163
MspA1I CmG'CkG
Cuts at: 0 459 571 1083
Size: 459 112 512
Fragments arranged by size:
512 459 112
NsiI A_TGCA'T
Cuts at: 0 634 1083
Size: 634 449
NspI r_CATG'y
Cuts at: 0 250 636 1083
Size: 250 386 447
Fragments arranged by size:
447 386 250
PflMI CCAn_nnn'nTGG
Cuts at: 0 999 1083
Size: 999 84
PfoI T'CCnGG_A
Cuts at: 0 1049 1083
Size: 1049 34
PpuMI rG'GwC_Cy
Cuts at: 0 294 1083
Size: 294 789
PspOMI G'GGCC_C
Cuts at: 0 976 1083
Size: 976 107
PstI C_TGCA'G
Cuts at: 0 149 464 581 784 1083
Size: 149 315 117 203 299
Fragments arranged by size:
315 299 203 149 117
PvuII CAG'CTG
Cuts at: 0 459 571 1083
Size: 459 112 512
Fragments arranged by size:
512 459 112
SapI GCTCTTCn'nnn_
Cuts at: 0 441 1083
Size: 441 642
SexAI A'CCwGG_T
Cuts at: 0 272 1083
Size: 272 811
SfcI C'TryA_G
Cuts at: 0 145 460 577 780 1083
Size: 145 315 117 203 303
Fragments arranged by size:
315 303 203 145 117
StyI C'CwwG_G
Cuts at: 0 233 656 677 1083
Size: 233 423 21 406
Fragments arranged by size:
423 406 233 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 392 694 1083
Size: 392 302 389
Fragments arranged by size:
392 389 302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 294 326 1083
Size: 294 32 757
Fragments arranged by size:
757 294 32
XbaI T'CTAG_A
Cuts at: 0 852 1083
Size: 852 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 916 1047 1083
Size: 916 131 36
Fragments arranged by size:
916 131 36
Enzymes that do cut:
Acc65I AlwNI ApaI ApoI AvaI BanI BanII BbsI
BfrBI BlpI Bme1580I BmrI BpmI BsaI BseRI BseYI
BsgI BsmI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI
BstXI BstYI Bsu36I BtgZI BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I KpnI MscI MslI MspA1I NsiI
NspI PflMI PfoI PpuMI PspOMI PstI PvuII SapI
SexAI SfcI StyI TaqII TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XhoI XmaI XmnI ZraI