Restriction map for
pFN21AE4344
(Linear) (Six-base) MAPSORT of: KIEE4344.seq Check: 5354 from: 1 to: 1080
KIEE4344.orf
With 182 enzymes: SgfI *
July 3, 2012 18:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1080
Size: 863 217
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1080
Size: 135 945
ApaLI G'TGCA_C
Cuts at: 0 500 1080
Size: 500 580
ApoI r'AATT_y
Cuts at: 0 766 1080
Size: 766 314
BamHI G'GATC_C
Cuts at: 0 325 1080
Size: 325 755
BanII G_rGCy'C
Cuts at: 0 892 1080
Size: 892 188
BbsI GAAGACnn'nnnn_
Cuts at: 0 610 880 1080
Size: 610 270 200
Fragments arranged by size:
610 270 200
BglI GCCn_nnn'nGGC
Cuts at: 0 250 1080
Size: 250 830
Bme1580I G_kGCm'C
Cuts at: 0 504 1080
Size: 504 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 471 1080
Size: 471 609
BmtI G_CTAG'C
Cuts at: 0 491 1080
Size: 491 589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 679 902 927 1080
Size: 376 303 223 25 153
Fragments arranged by size:
376 303 223 153 25
Bpu10I CC'TnA_GC
Cuts at: 0 779 1080
Size: 779 301
BsaI GGTCTCn'nnnn_
Cuts at: 0 655 1080
Size: 655 425
BsaHI Gr'CG_yC
Cuts at: 0 242 1080
Size: 242 838
BsaWI w'CCGG_w
Cuts at: 0 1013 1080
Size: 1013 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 925 955 1080
Size: 925 30 125
Fragments arranged by size:
925 125 30
BsiHKAI G_wGCw'C
Cuts at: 0 504 1080
Size: 504 576
BsmI GAATG_Cn'
Cuts at: 0 814 1080
Size: 814 266
Bsp1286I G_dGCh'C
Cuts at: 0 504 892 1080
Size: 504 388 188
Fragments arranged by size:
504 388 188
BspEI T'CCGG_A
Cuts at: 0 1013 1080
Size: 1013 67
BspHI T'CATG_A
Cuts at: 0 818 1080
Size: 818 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 1043 1080
Size: 296 747 37
Fragments arranged by size:
747 296 37
BsrDI GCAATG_nn'
Cuts at: 0 183 1080
Size: 183 897
BstEII G'GTnAC_C
Cuts at: 0 543 1080
Size: 543 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 657 1080
Size: 657 423
BstYI r'GATC_y
Cuts at: 0 325 1080
Size: 325 755
Bsu36I CC'TnA_GG
Cuts at: 0 293 1080
Size: 293 787
BtsI GCAGTG_nn'
Cuts at: 0 564 1080
Size: 564 516
DraI TTT'AAA
Cuts at: 0 399 1080
Size: 399 681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 679 863 902 927 1080
Size: 376 303 184 39 25 153
Fragments arranged by size:
376 303 184 153 39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 1080
Size: 292 788
EcoO109I rG'GnC_Cy
Cuts at: 0 1026 1080
Size: 1026 54
EcoRV GAT'ATC
Cuts at: 0 639 1080
Size: 639 441
HaeII r_GCGC'y
Cuts at: 0 98 1080
Size: 98 982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 252 284 879 911 1045 1077
Size: 25 32 195 32 595 32 134 32
Cuts at: 1077 1080
Size: 3
Fragments arranged by size:
595 195 134 32 32 32 32 25
3
HincII GTy'rAC
Cuts at: 0 444 1080
Size: 444 636
MspA1I CmG'CkG
Cuts at: 0 844 922 1080
Size: 844 78 158
Fragments arranged by size:
844 158 78
NheI G'CTAG_C
Cuts at: 0 487 1080
Size: 487 593
NspI r_CATG'y
Cuts at: 0 54 1080
Size: 54 1026
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1080
Size: 483 597
PfoI T'CCnGG_A
Cuts at: 0 43 61 328 1080
Size: 43 18 267 752
Fragments arranged by size:
752 267 43 18
PpuMI rG'GwC_Cy
Cuts at: 0 1026 1080
Size: 1026 54
PvuII CAG'CTG
Cuts at: 0 844 922 1080
Size: 844 78 158
Fragments arranged by size:
844 158 78
SfcI C'TryA_G
Cuts at: 0 102 1080
Size: 102 978
StyI C'CwwG_G
Cuts at: 0 482 1046 1080
Size: 482 564 34
Fragments arranged by size:
564 482 34
TatI w'GTAC_w
Cuts at: 0 410 1080
Size: 410 670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 724 1080
Size: 724 356
XmnI GAAnn'nnTTC
Cuts at: 0 767 1080
Size: 767 313
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI BamHI BanII BbsI BglI
Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BstEII BstXI BstYI Bsu36I BtsI DraI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I HincII MspA1I NheI NspI
PflMI PfoI PpuMI PvuII SfcI StyI TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BplI BpuEI
BsaAI BsaBI BseRI BseYI BsgI BsiEI BsiWI BsmBI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRI FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI ZraI