Restriction map for
pFN21AE4343
(Linear) (Six-base) MAPSORT of: KIEE4343.seq Check: 339 from: 1 to: 1062
KIEE4343.orf
With 182 enzymes: SgfI *
March 22, 2012 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 365 1062
Size: 365 697
AccI GT'mk_AC
Cuts at: 0 854 1062
Size: 854 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 1062
Size: 543 519
AflIII A'CryG_T
Cuts at: 0 573 1062
Size: 573 489
AlwNI CAG_nnn'CTG
Cuts at: 0 750 1062
Size: 750 312
ApoI r'AATT_y
Cuts at: 0 731 1062
Size: 731 331
AvaI C'yCGr_G
Cuts at: 0 220 606 1062
Size: 220 386 456
Fragments arranged by size:
456 386 220
BanII G_rGCy'C
Cuts at: 0 532 1062
Size: 532 530
BclI T'GATC_A
Cuts at: 0 809 1062
Size: 809 253
BglII A'GATC_T
Cuts at: 0 554 1062
Size: 554 508
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 372 1062
Size: 70 302 690
Fragments arranged by size:
690 302 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 546 1062
Size: 546 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 310 1062
Size: 178 132 752
Fragments arranged by size:
752 178 132
BsaHI Gr'CG_yC
Cuts at: 0 203 467 1062
Size: 203 264 595
Fragments arranged by size:
595 264 203
BsaWI w'CCGG_w
Cuts at: 0 633 1062
Size: 633 429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 163 337 343 415 759 1062
Size: 1 162 174 6 72 344 303
Fragments arranged by size:
344 303 174 162 72 6 1
BsiEI CG_ry'CG
Cuts at: 0 29 1062
Size: 29 1033
BsiHKAI G_wGCw'C
Cuts at: 0 35 398 704 1062
Size: 35 363 306 358
Fragments arranged by size:
363 358 306 35
BsmI GAATG_Cn'
Cuts at: 0 598 935 1062
Size: 598 337 127
Fragments arranged by size:
598 337 127
Bsp1286I G_dGCh'C
Cuts at: 0 35 398 532 704 1062
Size: 35 363 134 172 358
Fragments arranged by size:
363 358 172 134 35
BspEI T'CCGG_A
Cuts at: 0 633 1062
Size: 633 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 305 365 816 1062
Size: 185 120 60 451 246
Fragments arranged by size:
451 246 185 120 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1041 1062
Size: 1041 21
BstYI r'GATC_y
Cuts at: 0 554 1062
Size: 554 508
BstZ17I GTA'TAC
Cuts at: 0 855 1062
Size: 855 207
BtsI GCAGTG_nn'
Cuts at: 0 473 1062
Size: 473 589
EaeI y'GGCC_r
Cuts at: 0 26 276 1062
Size: 26 250 786
Fragments arranged by size:
786 250 26
EagI C'GGCC_G
Cuts at: 0 26 1062
Size: 26 1036
EarI CTCTTCn'nnn_
Cuts at: 0 617 982 1062
Size: 617 365 80
Fragments arranged by size:
617 365 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 310 543 1062
Size: 178 132 233 519
Fragments arranged by size:
519 233 178 132
EcoO109I rG'GnC_Cy
Cuts at: 0 153 321 668 1062
Size: 153 168 347 394
Fragments arranged by size:
394 347 168 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 646 1062
Size: 646 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 546 578 794 826 1062
Size: 546 32 216 32 236
Fragments arranged by size:
546 236 216 32 32
MscI TGG'CCA
Cuts at: 0 278 1062
Size: 278 784
MslI CAynn'nnrTG
Cuts at: 0 523 781 1039 1062
Size: 523 258 258 23
Fragments arranged by size:
523 258 258 23
MspA1I CmG'CkG
Cuts at: 0 351 407 646 747 1062
Size: 351 56 239 101 315
Fragments arranged by size:
351 315 239 101 56
NspI r_CATG'y
Cuts at: 0 312 577 1062
Size: 312 265 485
Fragments arranged by size:
485 312 265
PciI A'CATG_T
Cuts at: 0 573 1062
Size: 573 489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1062
Size: 286 32 744
Fragments arranged by size:
744 286 32
PpuMI rG'GwC_Cy
Cuts at: 0 668 1062
Size: 668 394
PsiI TTA'TAA
Cuts at: 0 446 1062
Size: 446 616
PspXI vC'TCGA_Gb
Cuts at: 0 220 1062
Size: 220 842
PstI C_TGCA'G
Cuts at: 0 164 1062
Size: 164 898
PvuII CAG'CTG
Cuts at: 0 351 747 1062
Size: 351 396 315
Fragments arranged by size:
396 351 315
SanDI GG'GwC_CC
Cuts at: 0 668 1062
Size: 668 394
SapI GCTCTTCn'nnn_
Cuts at: 0 617 1062
Size: 617 445
SfcI C'TryA_G
Cuts at: 0 160 336 1062
Size: 160 176 726
Fragments arranged by size:
726 176 160
SmaI CCC'GGG
Cuts at: 0 608 1062
Size: 608 454
SmlI C'TyrA_G
Cuts at: 0 220 1062
Size: 220 842
SspI AAT'ATT
Cuts at: 0 652 1062
Size: 652 410
StuI AGG'CCT
Cuts at: 0 497 1062
Size: 497 565
StyI C'CwwG_G
Cuts at: 0 228 1062
Size: 228 834
TatI w'GTAC_w
Cuts at: 0 581 770 883 1062
Size: 581 189 113 179
Fragments arranged by size:
581 189 179 113
Tth111I GACn'n_nGTC
Cuts at: 0 899 1062
Size: 899 163
XhoI C'TCGA_G
Cuts at: 0 220 1062
Size: 220 842
XmaI C'CCGG_G
Cuts at: 0 606 1062
Size: 606 456
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AvaI BanII
BclI BglII BmrI BplI BpmI BsaHI BsaWI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BstXI BstYI
BstZ17I BtsI EaeI EagI EarI Eco57MI EcoO109I FalI
Hin4I MscI MslI MspA1I NspI PciI PpiI PpuMI
PsiI PspXI PstI PvuII SanDI SapI SfcI SmaI
SmlI SspI StuI StyI TatI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiWI BsmBI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PshAI PspOMI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI TaqII TstI
XbaI XcmI XmnI ZraI