Restriction map for
pFN21AE4340
(Linear) (Six-base) MAPSORT of: KIEE4340.seq Check: 7131 from: 1 to: 1053
KIEE4340.orf
With 182 enzymes: SgfI *
March 22, 2012 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 447 1053
Size: 447 606
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1053
Size: 88 965
AfeI AGC'GCT
Cuts at: 0 358 1053
Size: 358 695
AleI CACnn'nnGTG
Cuts at: 0 935 1053
Size: 935 118
AvaI C'yCGr_G
Cuts at: 0 767 1053
Size: 767 286
BanII G_rGCy'C
Cuts at: 0 374 449 1053
Size: 374 75 604
Fragments arranged by size:
604 374 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 933 1053
Size: 933 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 748 782 958 992 1053
Size: 748 34 176 34 61
Fragments arranged by size:
748 176 61 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 181 1053
Size: 181 872
BlpI GC'TnA_GC
Cuts at: 0 82 368 443 1053
Size: 82 286 75 610
Fragments arranged by size:
610 286 82 75
Bme1580I G_kGCm'C
Cuts at: 0 380 628 1053
Size: 380 248 425
Fragments arranged by size:
425 380 248
BmrI ACTGGGnnnn_n'
Cuts at: 0 607 1053
Size: 607 446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 655 875 889 1053
Size: 589 66 220 14 164
Fragments arranged by size:
589 220 164 66 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 946 1053
Size: 205 741 107
Fragments arranged by size:
741 205 107
BsaBI GATnn'nnATC
Cuts at: 0 529 1053
Size: 529 524
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 958 988 1053
Size: 343 30 585 30 65
Fragments arranged by size:
585 343 65 30 30
BseYI C'CCAG_C
Cuts at: 0 1010 1053
Size: 1010 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 334 573 860 1053
Size: 130 204 239 287 193
Fragments arranged by size:
287 239 204 193 130
BsiHKAI G_wGCw'C
Cuts at: 0 83 154 374 449 890 1053
Size: 83 71 220 75 441 163
Fragments arranged by size:
441 220 163 83 75 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 1053
Size: 112 941
Bsp1286I G_dGCh'C
Cuts at: 0 83 154 374 380 449 628 890 1053
Size: 83 71 220 6 69 179 262 163
Fragments arranged by size:
262 220 179 163 83 71 69 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 832 1053
Size: 447 385 221
Fragments arranged by size:
447 385 221
BsrDI GCAATG_nn'
Cuts at: 0 425 1053
Size: 425 628
BsrFI r'CCGG_y
Cuts at: 0 215 1053
Size: 215 838
BssSI C'ACGA_G
Cuts at: 0 373 889 1053
Size: 373 516 164
Fragments arranged by size:
516 373 164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 1053
Size: 465 588
Bsu36I CC'TnA_GG
Cuts at: 0 746 1053
Size: 746 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 348 1053
Size: 348 705
BtsI GCAGTG_nn'
Cuts at: 0 118 1053
Size: 118 935
ClaI AT'CG_AT
Cuts at: 0 209 1053
Size: 209 844
DraIII CAC_nnn'GTG
Cuts at: 0 730 1053
Size: 730 323
EaeI y'GGCC_r
Cuts at: 0 173 800 1053
Size: 173 627 253
Fragments arranged by size:
627 253 173
EarI CTCTTCn'nnn_
Cuts at: 0 835 1053
Size: 835 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 235 1053
Size: 235 818
EcoICRI GAG'CTC
Cuts at: 0 372 447 1053
Size: 372 75 606
Fragments arranged by size:
606 372 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 589 655 875 889 1053
Size: 88 501 66 220 14 164
Fragments arranged by size:
501 220 164 88 66 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 1053
Size: 556 497
EcoO109I rG'GnC_Cy
Cuts at: 0 513 750 1053
Size: 513 237 303
Fragments arranged by size:
513 303 237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 930 1053
Size: 846 84 123
Fragments arranged by size:
846 123 84
HaeII r_GCGC'y
Cuts at: 0 360 1017 1053
Size: 360 657 36
Fragments arranged by size:
657 360 36
MscI TGG'CCA
Cuts at: 0 175 1053
Size: 175 878
MslI CAynn'nnrTG
Cuts at: 0 266 463 935 1053
Size: 266 197 472 118
Fragments arranged by size:
472 266 197 118
MspA1I CmG'CkG
Cuts at: 0 762 1053
Size: 762 291
PflMI CCAn_nnn'nTGG
Cuts at: 0 730 1053
Size: 730 323
PfoI T'CCnGG_A
Cuts at: 0 11 491 632 1053
Size: 11 480 141 421
Fragments arranged by size:
480 421 141 11
PpuMI rG'GwC_Cy
Cuts at: 0 513 1053
Size: 513 540
PstI C_TGCA'G
Cuts at: 0 149 1053
Size: 149 904
PvuII CAG'CTG
Cuts at: 0 762 1053
Size: 762 291
SacI G_AGCT'C
Cuts at: 0 374 449 1053
Size: 374 75 604
Fragments arranged by size:
604 374 75
SapI GCTCTTCn'nnn_
Cuts at: 0 835 1053
Size: 835 218
SexAI A'CCwGG_T
Cuts at: 0 104 1053
Size: 104 949
SfcI C'TryA_G
Cuts at: 0 145 1053
Size: 145 908
SmlI C'TyrA_G
Cuts at: 0 220 961 1053
Size: 220 741 92
Fragments arranged by size:
741 220 92
StuI AGG'CCT
Cuts at: 0 562 1053
Size: 562 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 890 1053
Size: 404 486 163
Fragments arranged by size:
486 404 163
TatI w'GTAC_w
Cuts at: 0 818 1053
Size: 818 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 958 990 1053
Size: 343 32 583 32 63
Fragments arranged by size:
583 343 63 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 198 1053
Size: 198 855
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 931 1053
Size: 931 122
XmnI GAAnn'nnTTC
Cuts at: 0 925 1053
Size: 925 128
Enzymes that do cut:
AarI AcuI AfeI AleI AvaI BanII BbsI BcgI
BglI BlpI Bme1580I BmrI BpmI BpuEI BsaBI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstXI Bsu36I BtgZI BtsI ClaI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
MscI MslI MspA1I PflMI PfoI PpuMI PstI PvuII
SacI SapI SexAI SfcI SmlI StuI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstYI
BstZ17I BtgI CspCI DraI DrdI EagI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI XbaI XhoI XmaI ZraI