Restriction map for
pFN21AE4337
(Linear) (Six-base) MAPSORT of: KIEE4337.seq Check: 9948 from: 1 to: 1044
KIEE4337.orf
With 182 enzymes: SgfI *
March 22, 2012 17:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1044
Size: 1007 37
AflII C'TTAA_G
Cuts at: 0 762 1044
Size: 762 282
AlwNI CAG_nnn'CTG
Cuts at: 0 162 345 400 423 1044
Size: 162 183 55 23 621
Fragments arranged by size:
621 183 162 55 23
BamHI G'GATC_C
Cuts at: 0 597 1044
Size: 597 447
BanI G'GyrC_C
Cuts at: 0 568 1044
Size: 568 476
BanII G_rGCy'C
Cuts at: 0 130 216 272 1044
Size: 130 86 56 772
Fragments arranged by size:
772 130 86 56
BlpI GC'TnA_GC
Cuts at: 0 393 1044
Size: 393 651
Bme1580I G_kGCm'C
Cuts at: 0 573 811 817 1044
Size: 573 238 6 227
Fragments arranged by size:
573 238 227 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 411 1044
Size: 411 633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 876 1044
Size: 876 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 88 367 1044
Size: 79 9 279 677
Fragments arranged by size:
677 279 79 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1044
Size: 256 788
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 940 970 1044
Size: 940 30 74
Fragments arranged by size:
940 74 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 961 1044
Size: 202 759 83
Fragments arranged by size:
759 202 83
BseYI C'CCAG_C
Cuts at: 0 931 951 1044
Size: 931 20 93
Fragments arranged by size:
931 93 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1044
Size: 313 731
BsiEI CG_ry'CG
Cuts at: 0 650 1044
Size: 650 394
BsmBI CGTCTCn'nnnn_
Cuts at: 0 229 1044
Size: 229 815
Bsp1286I G_dGCh'C
Cuts at: 0 130 216 272 573 811 817 1044
Size: 130 86 56 301 238 6 227
Fragments arranged by size:
301 238 227 130 86 56 6
BspHI T'CATG_A
Cuts at: 0 353 1044
Size: 353 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 431 1044
Size: 431 613
BsrBI CCG'CTC
Cuts at: 0 173 1044
Size: 173 871
BsrDI GCAATG_nn'
Cuts at: 0 687 752 1044
Size: 687 65 292
Fragments arranged by size:
687 292 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 580 1044
Size: 580 464
BstYI r'GATC_y
Cuts at: 0 597 735 794 872 993 1044
Size: 597 138 59 78 121 51
Fragments arranged by size:
597 138 121 78 59 51
Bsu36I CC'TnA_GG
Cuts at: 0 969 1044
Size: 969 75
BtgI C'CryG_G
Cuts at: 0 117 278 363 446 1044
Size: 117 161 85 83 598
Fragments arranged by size:
598 161 117 85 83
BtsI GCAGTG_nn'
Cuts at: 0 545 1044
Size: 545 499
ClaI AT'CG_AT
Cuts at: 0 95 1044
Size: 95 949
DraIII CAC_nnn'GTG
Cuts at: 0 54 369 1044
Size: 54 315 675
Fragments arranged by size:
675 315 54
EaeI y'GGCC_r
Cuts at: 0 740 1044
Size: 740 304
EarI CTCTTCn'nnn_
Cuts at: 0 232 1044
Size: 232 812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 88 367 1007 1044
Size: 79 9 279 640 37
Fragments arranged by size:
640 279 79 37 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 514 1044
Size: 433 81 530
Fragments arranged by size:
530 433 81
EcoO109I rG'GnC_Cy
Cuts at: 0 507 663 1044
Size: 507 156 381
Fragments arranged by size:
507 381 156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 1044
Size: 258 786
HaeII r_GCGC'y
Cuts at: 0 1005 1044
Size: 1005 39
HincII GTy'rAC
Cuts at: 0 321 1044
Size: 321 723
HpaI GTT'AAC
Cuts at: 0 321 1044
Size: 321 723
MscI TGG'CCA
Cuts at: 0 742 1044
Size: 742 302
PasI CC'CwG_GG
Cuts at: 0 603 1044
Size: 603 441
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 732 849 1044
Size: 345 387 117 195
Fragments arranged by size:
387 345 195 117
PfoI T'CCnGG_A
Cuts at: 0 155 503 713 1044
Size: 155 348 210 331
Fragments arranged by size:
348 331 210 155
PpuMI rG'GwC_Cy
Cuts at: 0 507 1044
Size: 507 537
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 403 435 1044
Size: 403 32 609
Fragments arranged by size:
609 403 32
PstI C_TGCA'G
Cuts at: 0 836 1044
Size: 836 208
PvuI CG_AT'CG
Cuts at: 0 650 1044
Size: 650 394
SexAI A'CCwGG_T
Cuts at: 0 377 1044
Size: 377 667
SfcI C'TryA_G
Cuts at: 0 217 832 1044
Size: 217 615 212
Fragments arranged by size:
615 217 212
SmlI C'TyrA_G
Cuts at: 0 271 762 1044
Size: 271 491 282
Fragments arranged by size:
491 282 271
StuI AGG'CCT
Cuts at: 0 859 1044
Size: 859 185
StyI C'CwwG_G
Cuts at: 0 743 1044
Size: 743 301
TatI w'GTAC_w
Cuts at: 0 236 1044
Size: 236 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 940 970 972 1002 1044
Size: 940 30 2 30 42
Fragments arranged by size:
940 42 30 30 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 448 933 1044
Size: 342 106 485 111
Fragments arranged by size:
485 342 111 106
Enzymes that do cut:
AcuI AflII AlwNI BamHI BanI BanII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaXI BseRI BseYI BsgI
BsiEI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstXI
BstYI Bsu36I BtgI BtsI ClaI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I FalI HaeII HincII HpaI MscI
PasI PflMI PfoI PpuMI PsrI PstI PvuI SexAI
SfcI SmlI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI
BsmI BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI CspCI DraI DrdI EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII
KasI KpnI MfeI MluI MmeI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI