Restriction map for
pFN21AE4331
(Linear) (Six-base) MAPSORT of: KIEE4331.seq Check: 9984 from: 1 to: 1014
KIEE4331.orf
With 182 enzymes: SgfI *
July 3, 2012 18:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 151 571 988 1014
Size: 33 118 420 417 26
Fragments arranged by size:
420 417 118 33 26
AfeI AGC'GCT
Cuts at: 0 30 1014
Size: 30 984
AleI CACnn'nnGTG
Cuts at: 0 358 706 1014
Size: 358 348 308
Fragments arranged by size:
358 348 308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 1014
Size: 322 32 660
Fragments arranged by size:
660 322 32
AlwNI CAG_nnn'CTG
Cuts at: 0 736 1014
Size: 736 278
ApaLI G'TGCA_C
Cuts at: 0 633 1014
Size: 633 381
AvaI C'yCGr_G
Cuts at: 0 326 569 961 1014
Size: 326 243 392 53
Fragments arranged by size:
392 326 243 53
BamHI G'GATC_C
Cuts at: 0 561 686 1014
Size: 561 125 328
Fragments arranged by size:
561 328 125
BanI G'GyrC_C
Cuts at: 0 763 1014
Size: 763 251
BbeI G_GCGC'C
Cuts at: 0 767 1014
Size: 767 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 613 768 1009 1014
Size: 613 155 241 5
Fragments arranged by size:
613 241 155 5
BglII A'GATC_T
Cuts at: 0 198 1014
Size: 198 816
Bme1580I G_kGCm'C
Cuts at: 0 387 637 1014
Size: 387 250 377
Fragments arranged by size:
387 377 250
BmrI ACTGGGnnnn_n'
Cuts at: 0 689 1014
Size: 689 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 320 1014
Size: 119 201 694
Fragments arranged by size:
694 201 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 1014
Size: 208 806
BsaHI Gr'CG_yC
Cuts at: 0 764 1014
Size: 764 250
BsaWI w'CCGG_w
Cuts at: 0 426 1014
Size: 426 588
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 915 949 1014
Size: 915 34 65
Fragments arranged by size:
915 65 34
BseYI C'CCAG_C
Cuts at: 0 386 601 1014
Size: 386 215 413
Fragments arranged by size:
413 386 215
BsiHKAI G_wGCw'C
Cuts at: 0 637 1014
Size: 637 377
Bsp1286I G_dGCh'C
Cuts at: 0 387 637 1014
Size: 387 250 377
Fragments arranged by size:
387 377 250
BsrDI GCAATG_nn'
Cuts at: 0 416 800 1014
Size: 416 384 214
Fragments arranged by size:
416 384 214
BsrFI r'CCGG_y
Cuts at: 0 697 760 1014
Size: 697 63 254
Fragments arranged by size:
697 254 63
BsrGI T'GTAC_A
Cuts at: 0 479 1014
Size: 479 535
BstAPI GCAn_nnn'nTGC
Cuts at: 0 736 1014
Size: 736 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1014
Size: 360 654
BstYI r'GATC_y
Cuts at: 0 198 561 686 1014
Size: 198 363 125 328
Fragments arranged by size:
363 328 198 125
BtgI C'CryG_G
Cuts at: 0 749 1014
Size: 749 265
BtsI GCAGTG_nn'
Cuts at: 0 390 1014
Size: 390 624
EaeI y'GGCC_r
Cuts at: 0 191 362 594 1014
Size: 191 171 232 420
Fragments arranged by size:
420 232 191 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 699 1014
Size: 28 671 315
Fragments arranged by size:
671 315 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 119 151 320 571 988 1014
Size: 33 86 32 169 251 417 26
Fragments arranged by size:
417 251 169 86 33 32 26
EcoO109I rG'GnC_Cy
Cuts at: 0 92 1014
Size: 92 922
EcoRV GAT'ATC
Cuts at: 0 171 1014
Size: 171 843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 1014
Size: 666 348
HaeII r_GCGC'y
Cuts at: 0 32 767 1014
Size: 32 735 247
Fragments arranged by size:
735 247 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 1014
Size: 563 32 419
Fragments arranged by size:
563 419 32
HincII GTy'rAC
Cuts at: 0 576 1014
Size: 576 438
KasI G'GCGC_C
Cuts at: 0 763 1014
Size: 763 251
MscI TGG'CCA
Cuts at: 0 364 596 1014
Size: 364 232 418
Fragments arranged by size:
418 364 232
MslI CAynn'nnrTG
Cuts at: 0 358 706 1014
Size: 358 348 308
Fragments arranged by size:
358 348 308
MspA1I CmG'CkG
Cuts at: 0 226 255 376 666 873 1014
Size: 226 29 121 290 207 141
Fragments arranged by size:
290 226 207 141 121 29
NaeI GCC'GGC
Cuts at: 0 699 762 1014
Size: 699 63 252
Fragments arranged by size:
699 252 63
NarI GG'CG_CC
Cuts at: 0 764 1014
Size: 764 250
NcoI C'CATG_G
Cuts at: 0 749 1014
Size: 749 265
NgoMIV G'CCGG_C
Cuts at: 0 697 760 1014
Size: 697 63 254
Fragments arranged by size:
697 254 63
PfoI T'CCnGG_A
Cuts at: 0 689 1014
Size: 689 325
PspXI vC'TCGA_Gb
Cuts at: 0 569 1014
Size: 569 445
PvuII CAG'CTG
Cuts at: 0 255 666 1014
Size: 255 411 348
Fragments arranged by size:
411 348 255
SfoI GGC'GCC
Cuts at: 0 765 1014
Size: 765 249
SmaI CCC'GGG
Cuts at: 0 328 1014
Size: 328 686
SmlI C'TyrA_G
Cuts at: 0 569 1014
Size: 569 445
StuI AGG'CCT
Cuts at: 0 973 1014
Size: 973 41
StyI C'CwwG_G
Cuts at: 0 143 641 749 1014
Size: 143 498 108 265
Fragments arranged by size:
498 265 143 108
TatI w'GTAC_w
Cuts at: 0 479 1014
Size: 479 535
Tth111I GACn'n_nGTC
Cuts at: 0 520 1014
Size: 520 494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 747 1014
Size: 747 267
XhoI C'TCGA_G
Cuts at: 0 569 1014
Size: 569 445
XmaI C'CCGG_G
Cuts at: 0 326 1014
Size: 326 688
Enzymes that do cut:
AcuI AfeI AleI AloI AlwNI ApaLI AvaI BamHI
BanI BbeI BbsI BglII Bme1580I BmrI BpmI BsaI
BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I BsrDI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtsI EaeI EciI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PfoI
PspXI PvuII SfoI SmaI SmlI StuI StyI TatI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI XbaI XmnI ZraI