Restriction map for
pFN21AE4328
(Linear) (Six-base) MAPSORT of: KIEE4328.seq Check: 6009 from: 1 to: 1002
KIEE4328.orf
With 182 enzymes: SgfI *
March 22, 2012 17:44 ..
Acc65I G'GTAC_C
Cuts at: 0 247 371 1002
Size: 247 124 631
Fragments arranged by size:
631 247 124
AflII C'TTAA_G
Cuts at: 0 774 1002
Size: 774 228
AflIII A'CryG_T
Cuts at: 0 241 1002
Size: 241 761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1002
Size: 34 32 936
Fragments arranged by size:
936 34 32
AlwNI CAG_nnn'CTG
Cuts at: 0 646 1002
Size: 646 356
ApoI r'AATT_y
Cuts at: 0 345 384 456 533 623 745 835 1002
Size: 345 39 72 77 90 122 90 167
Fragments arranged by size:
345 167 122 90 90 77 72 39
BanI G'GyrC_C
Cuts at: 0 247 371 1002
Size: 247 124 631
Fragments arranged by size:
631 247 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 244 813 1002
Size: 244 569 189
Fragments arranged by size:
569 244 189
BclI T'GATC_A
Cuts at: 0 800 1002
Size: 800 202
BglII A'GATC_T
Cuts at: 0 109 1002
Size: 109 893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 719 1002
Size: 61 658 283
Fragments arranged by size:
658 283 61
BsaWI w'CCGG_w
Cuts at: 0 380 1002
Size: 380 622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 1002
Size: 34 30 938
Fragments arranged by size:
938 34 30
BseYI C'CCAG_C
Cuts at: 0 555 1002
Size: 555 447
BsmBI CGTCTCn'nnnn_
Cuts at: 0 216 1002
Size: 216 786
BspHI T'CATG_A
Cuts at: 0 350 1002
Size: 350 652
BsrDI GCAATG_nn'
Cuts at: 0 148 1002
Size: 148 854
BssSI C'ACGA_G
Cuts at: 0 611 1002
Size: 611 391
BstBI TT'CG_AA
Cuts at: 0 20 1002
Size: 20 982
BstXI CCAn_nnnn'nTGG
Cuts at: 0 85 1002
Size: 85 917
BstYI r'GATC_y
Cuts at: 0 82 90 109 1002
Size: 82 8 19 893
Fragments arranged by size:
893 82 19 8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 657 692 834 869 1002
Size: 657 35 142 35 133
Fragments arranged by size:
657 142 133 35 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 719 1002
Size: 61 658 283
Fragments arranged by size:
658 283 61
EcoRI G'AATT_C
Cuts at: 0 456 623 1002
Size: 456 167 379
Fragments arranged by size:
456 379 167
HindIII A'AGCT_T
Cuts at: 0 182 286 324 1002
Size: 182 104 38 678
Fragments arranged by size:
678 182 104 38
KpnI G_GTAC'C
Cuts at: 0 251 375 1002
Size: 251 124 627
Fragments arranged by size:
627 251 124
MslI CAynn'nnrTG
Cuts at: 0 157 1002
Size: 157 845
NspI r_CATG'y
Cuts at: 0 210 245 1002
Size: 210 35 757
Fragments arranged by size:
757 210 35
PciI A'CATG_T
Cuts at: 0 241 1002
Size: 241 761
PfoI T'CCnGG_A
Cuts at: 0 389 1002
Size: 389 613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1002
Size: 34 32 936
Fragments arranged by size:
936 34 32
SexAI A'CCwGG_T
Cuts at: 0 639 1002
Size: 639 363
SmlI C'TyrA_G
Cuts at: 0 774 1002
Size: 774 228
StyI C'CwwG_G
Cuts at: 0 78 366 527 1002
Size: 78 288 161 475
Fragments arranged by size:
475 288 161 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 1002
Size: 43 959
TatI w'GTAC_w
Cuts at: 0 189 1002
Size: 189 813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1002
Size: 824 32 146
Fragments arranged by size:
824 146 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 113 1002
Size: 113 889
Enzymes that do cut:
Acc65I AflII AflIII AloI AlwNI ApoI BanI BbsI
BclI BglII BpmI BsaWI BsaXI BseYI BsmBI BspHI
BsrDI BssSI BstBI BstXI BstYI CspCI Eco57MI EcoRI
HindIII KpnI MslI NspI PciI PfoI PpiI SexAI
SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI