Restriction map for
pFN21AE4327
(Linear) (Six-base) MAPSORT of: KIEE4327.seq Check: 2729 from: 1 to: 996
KIEE4327.orf
With 182 enzymes: SgfI *
March 22, 2012 17:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 283 996
Size: 283 713
AleI CACnn'nnGTG
Cuts at: 0 383 414 996
Size: 383 31 582
Fragments arranged by size:
582 383 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 432 996
Size: 432 564
AlwNI CAG_nnn'CTG
Cuts at: 0 39 347 996
Size: 39 308 649
Fragments arranged by size:
649 308 39
ApaI G_GGCC'C
Cuts at: 0 119 977 996
Size: 119 858 19
Fragments arranged by size:
858 119 19
ApaLI G'TGCA_C
Cuts at: 0 407 996
Size: 407 589
BanI G'GyrC_C
Cuts at: 0 178 996
Size: 178 818
BanII G_rGCy'C
Cuts at: 0 119 340 977 996
Size: 119 221 637 19
Fragments arranged by size:
637 221 119 19
BbvCI CC'TCA_GC
Cuts at: 0 120 996
Size: 120 876
BfrBI ATG'CAT
Cuts at: 0 3 996
Size: 3 993
BglI GCCn_nnn'nGGC
Cuts at: 0 981 996
Size: 981 15
Bme1580I G_kGCm'C
Cuts at: 0 119 181 411 977 996
Size: 119 62 230 566 19
Fragments arranged by size:
566 230 119 62 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 996
Size: 120 876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 591 787 996
Size: 324 267 196 209
Fragments arranged by size:
324 267 209 196
Bpu10I CC'TnA_GC
Cuts at: 0 120 996
Size: 120 876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 218 248 564 594 996
Size: 218 30 316 30 402
Fragments arranged by size:
402 316 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 799 802 996
Size: 343 456 3 194
Fragments arranged by size:
456 343 194 3
BseYI C'CCAG_C
Cuts at: 0 327 659 996
Size: 327 332 337
Fragments arranged by size:
337 332 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 192 996
Size: 77 115 804
Fragments arranged by size:
804 115 77
BsiHKAI G_wGCw'C
Cuts at: 0 411 439 996
Size: 411 28 557
Fragments arranged by size:
557 411 28
Bsp1286I G_dGCh'C
Cuts at: 0 119 181 340 411 439 977 996
Size: 119 62 159 71 28 538 19
Fragments arranged by size:
538 159 119 71 62 28 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 283 485 529 996
Size: 283 202 44 467
Fragments arranged by size:
467 283 202 44
BsrFI r'CCGG_y
Cuts at: 0 984 996
Size: 984 12
BssSI C'ACGA_G
Cuts at: 0 438 534 996
Size: 438 96 462
Fragments arranged by size:
462 438 96
BstAPI GCAn_nnn'nTGC
Cuts at: 0 406 996
Size: 406 590
BstEII G'GTnAC_C
Cuts at: 0 684 996
Size: 684 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 996
Size: 369 627
Bsu36I CC'TnA_GG
Cuts at: 0 163 427 996
Size: 163 264 569
Fragments arranged by size:
569 264 163
BtgI C'CryG_G
Cuts at: 0 57 977 996
Size: 57 920 19
Fragments arranged by size:
920 57 19
BtsI GCAGTG_nn'
Cuts at: 0 37 407 439 473 555 996
Size: 37 370 32 34 82 441
Fragments arranged by size:
441 370 82 37 34 32
ClaI AT'CG_AT
Cuts at: 0 302 996
Size: 302 694
EaeI y'GGCC_r
Cuts at: 0 552 758 880 996
Size: 552 206 122 116
Fragments arranged by size:
552 206 122 116
EarI CTCTTCn'nnn_
Cuts at: 0 361 635 996
Size: 361 274 361
Fragments arranged by size:
361 361 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 591 787 996
Size: 324 267 196 209
Fragments arranged by size:
324 267 209 196
EcoO109I rG'GnC_Cy
Cuts at: 0 115 116 159 856 973 996
Size: 115 1 43 697 117 23
Fragments arranged by size:
697 117 115 43 23 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 407 996
Size: 407 589
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 564 596 996
Size: 218 32 314 32 400
Fragments arranged by size:
400 314 218 32 32
MscI TGG'CCA
Cuts at: 0 554 760 882 996
Size: 554 206 122 114
Fragments arranged by size:
554 206 122 114
MslI CAynn'nnrTG
Cuts at: 0 383 414 996
Size: 383 31 582
Fragments arranged by size:
582 383 31
NsiI A_TGCA'T
Cuts at: 0 5 996
Size: 5 991
NspI r_CATG'y
Cuts at: 0 482 996
Size: 482 514
PasI CC'CwG_GG
Cuts at: 0 872 996
Size: 872 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 347 996
Size: 347 649
PspOMI G'GGCC_C
Cuts at: 0 115 973 996
Size: 115 858 23
Fragments arranged by size:
858 115 23
PstI C_TGCA'G
Cuts at: 0 47 996
Size: 47 949
SfcI C'TryA_G
Cuts at: 0 43 996
Size: 43 953
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 981 996
Size: 981 15
StuI AGG'CCT
Cuts at: 0 646 996
Size: 646 350
StyI C'CwwG_G
Cuts at: 0 146 256 587 996
Size: 146 110 331 409
Fragments arranged by size:
409 331 146 110
XbaI T'CTAG_A
Cuts at: 0 262 996
Size: 262 734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 875 996
Size: 480 395 121
Fragments arranged by size:
480 395 121
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaI ApaLI BanI BanII
BbvCI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssSI
BstAPI BstEII BstXI Bsu36I BtgI BtsI ClaI EaeI
EarI Eco57MI EcoO109I FalI Hin4I MscI MslI NsiI
NspI PasI PflMI PspOMI PstI SfcI SfiI StuI
StyI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstYI BstZ17I
BtgZI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI TstI Tth111I XhoI XmaI XmnI
ZraI