Restriction map for
pFN21AE4324
(Linear) (Six-base) MAPSORT of: KIEE4324.seq Check: 5746 from: 1 to: 978
KIEE4324.orf
With 182 enzymes: SgfI *
July 3, 2012 18:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 978
Size: 187 791
AccI GT'mk_AC
Cuts at: 0 617 978
Size: 617 361
AflIII A'CryG_T
Cuts at: 0 94 978
Size: 94 884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 978
Size: 376 602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 212 244 978
Size: 212 32 734
Fragments arranged by size:
734 212 32
ApaI G_GGCC'C
Cuts at: 0 177 266 978
Size: 177 89 712
Fragments arranged by size:
712 177 89
AvaI C'yCGr_G
Cuts at: 0 26 159 169 978
Size: 26 133 10 809
Fragments arranged by size:
809 133 26 10
BanII G_rGCy'C
Cuts at: 0 130 177 240 266 611 978
Size: 130 47 63 26 345 367
Fragments arranged by size:
367 345 130 63 47 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 866 978
Size: 832 34 112
Fragments arranged by size:
832 112 34
BglI GCCn_nnn'nGGC
Cuts at: 0 22 125 978
Size: 22 103 853
Fragments arranged by size:
853 103 22
Bme1580I G_kGCm'C
Cuts at: 0 177 266 978
Size: 177 89 712
Fragments arranged by size:
712 177 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 715 978
Size: 522 193 263
Fragments arranged by size:
522 263 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 254 936 978
Size: 142 112 682 42
Fragments arranged by size:
682 142 112 42
Bpu10I CC'TnA_GC
Cuts at: 0 382 978
Size: 382 596
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 232 266 874 978
Size: 37 195 34 608 104
Fragments arranged by size:
608 195 104 37 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 212 242 701 731 751 781 978
Size: 212 30 459 30 20 30 197
Fragments arranged by size:
459 212 197 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 230 293 978
Size: 230 63 685
Fragments arranged by size:
685 230 63
BseYI C'CCAG_C
Cuts at: 0 313 546 561 978
Size: 313 233 15 417
Fragments arranged by size:
417 313 233 15
BsiHKAI G_wGCw'C
Cuts at: 0 434 611 978
Size: 434 177 367
Fragments arranged by size:
434 367 177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 302 978
Size: 302 676
Bsp1286I G_dGCh'C
Cuts at: 0 130 177 240 266 434 611 978
Size: 130 47 63 26 168 177 367
Fragments arranged by size:
367 177 168 130 63 47 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 187 368 413 978
Size: 80 107 181 45 565
Fragments arranged by size:
565 181 107 80 45
BsrBI CCG'CTC
Cuts at: 0 386 978
Size: 386 592
BsrFI r'CCGG_y
Cuts at: 0 416 978
Size: 416 562
BstBI TT'CG_AA
Cuts at: 0 802 978
Size: 802 176
BtgI C'CryG_G
Cuts at: 0 20 978
Size: 20 958
BtsI GCAGTG_nn'
Cuts at: 0 732 978
Size: 732 246
EcoICRI GAG'CTC
Cuts at: 0 609 978
Size: 609 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 254 936 978
Size: 142 112 682 42
Fragments arranged by size:
682 142 112 42
EcoO109I rG'GnC_Cy
Cuts at: 0 173 174 262 357 883 978
Size: 173 1 88 95 526 95
Fragments arranged by size:
526 173 95 95 88 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 549 978
Size: 549 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 701 733 978
Size: 701 32 245
Fragments arranged by size:
701 245 32
MspA1I CmG'CkG
Cuts at: 0 477 546 978
Size: 477 69 432
Fragments arranged by size:
477 432 69
NaeI GCC'GGC
Cuts at: 0 418 978
Size: 418 560
NcoI C'CATG_G
Cuts at: 0 20 978
Size: 20 958
NgoMIV G'CCGG_C
Cuts at: 0 416 978
Size: 416 562
NspI r_CATG'y
Cuts at: 0 98 978
Size: 98 880
PasI CC'CwG_GG
Cuts at: 0 178 594 978
Size: 178 416 384
Fragments arranged by size:
416 384 178
PciI A'CATG_T
Cuts at: 0 94 978
Size: 94 884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 978
Size: 212 32 734
Fragments arranged by size:
734 212 32
PpuMI rG'GwC_Cy
Cuts at: 0 357 883 978
Size: 357 526 95
Fragments arranged by size:
526 357 95
PshAI GACnn'nnGTC
Cuts at: 0 95 978
Size: 95 883
PspOMI G'GGCC_C
Cuts at: 0 173 262 978
Size: 173 89 716
Fragments arranged by size:
716 173 89
PvuII CAG'CTG
Cuts at: 0 546 978
Size: 546 432
SacI G_AGCT'C
Cuts at: 0 611 978
Size: 611 367
SanDI GG'GwC_CC
Cuts at: 0 883 978
Size: 883 95
SexAI A'CCwGG_T
Cuts at: 0 620 978
Size: 620 358
SmaI CCC'GGG
Cuts at: 0 28 161 171 978
Size: 28 133 10 807
Fragments arranged by size:
807 133 28 10
StyI C'CwwG_G
Cuts at: 0 20 978
Size: 20 958
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 978
Size: 290 688
TatI w'GTAC_w
Cuts at: 0 656 692 978
Size: 656 36 286
Fragments arranged by size:
656 286 36
XmaI C'CCGG_G
Cuts at: 0 26 159 169 978
Size: 26 133 10 809
Fragments arranged by size:
809 133 26 10
Enzymes that do cut:
AarI AccI AflIII AlfI AloI ApaI AvaI BanII
BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BstBI BtgI BtsI EcoICRI Eco57MI EcoO109I FalI Hin4I
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PpiI
PpuMI PshAI PspOMI PvuII SacI SanDI SexAI SmaI
StyI TaqII TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI