Restriction map for
pFN21AE4322
(Linear) (Six-base) MAPSORT of: KIEE4322.seq Check: 7288 from: 1 to: 975
KIEE4322.orf
With 182 enzymes: SgfI *
March 22, 2012 17:43 ..
AccI GT'mk_AC
Cuts at: 0 845 975
Size: 845 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 287 481 975
Size: 212 75 194 494
Fragments arranged by size:
494 212 194 75
AhdI GACnn_n'nnGTC
Cuts at: 0 651 975
Size: 651 324
AleI CACnn'nnGTG
Cuts at: 0 875 877 975
Size: 875 2 98
Fragments arranged by size:
875 98 2
ApoI r'AATT_y
Cuts at: 0 324 975
Size: 324 651
AvaI C'yCGr_G
Cuts at: 0 119 975
Size: 119 856
BanI G'GyrC_C
Cuts at: 0 625 975
Size: 625 350
BanII G_rGCy'C
Cuts at: 0 398 975
Size: 398 577
BbsI GAAGACnn'nnnn_
Cuts at: 0 842 975
Size: 842 133
BbvCI CC'TCA_GC
Cuts at: 0 557 975
Size: 557 418
BglII A'GATC_T
Cuts at: 0 536 596 975
Size: 536 60 379
Fragments arranged by size:
536 379 60
Bme1580I G_kGCm'C
Cuts at: 0 82 628 975
Size: 82 546 347
Fragments arranged by size:
546 347 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 975
Size: 304 671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 377 871 975
Size: 143 234 494 104
Fragments arranged by size:
494 234 143 104
Bpu10I CC'TnA_GC
Cuts at: 0 557 956 975
Size: 557 399 19
Fragments arranged by size:
557 399 19
BsaHI Gr'CG_yC
Cuts at: 0 46 975
Size: 46 929
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 746 776 975
Size: 583 30 133 30 199
Fragments arranged by size:
583 199 133 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 975
Size: 445 530
BseYI C'CCAG_C
Cuts at: 0 543 975
Size: 543 432
BsiHKAI G_wGCw'C
Cuts at: 0 398 513 975
Size: 398 115 462
Fragments arranged by size:
462 398 115
BsmI GAATG_Cn'
Cuts at: 0 281 866 975
Size: 281 585 109
Fragments arranged by size:
585 281 109
BsmBI CGTCTCn'nnnn_
Cuts at: 0 507 975
Size: 507 468
Bsp1286I G_dGCh'C
Cuts at: 0 82 398 513 628 975
Size: 82 316 115 115 347
Fragments arranged by size:
347 316 115 115 82
BspHI T'CATG_A
Cuts at: 0 638 975
Size: 638 337
BsrDI GCAATG_nn'
Cuts at: 0 14 975
Size: 14 961
BstAPI GCAn_nnn'nTGC
Cuts at: 0 349 672 975
Size: 349 323 303
Fragments arranged by size:
349 323 303
BstEII G'GTnAC_C
Cuts at: 0 256 561 975
Size: 256 305 414
Fragments arranged by size:
414 305 256
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 975
Size: 621 354
BstYI r'GATC_y
Cuts at: 0 536 596 975
Size: 536 60 379
Fragments arranged by size:
536 379 60
BtgI C'CryG_G
Cuts at: 0 25 975
Size: 25 950
BtsI GCAGTG_nn'
Cuts at: 0 696 975
Size: 696 279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 207 242 975
Size: 207 35 733
Fragments arranged by size:
733 207 35
EaeI y'GGCC_r
Cuts at: 0 725 827 975
Size: 725 102 148
Fragments arranged by size:
725 148 102
EarI CTCTTCn'nnn_
Cuts at: 0 403 777 975
Size: 403 374 198
Fragments arranged by size:
403 374 198
EcoICRI GAG'CTC
Cuts at: 0 396 975
Size: 396 579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 212 287 377 481 871 975
Size: 143 69 75 90 104 390 104
Fragments arranged by size:
390 143 104 104 90 75 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 527 975
Size: 527 448
HaeII r_GCGC'y
Cuts at: 0 22 975
Size: 22 953
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 746 778 975
Size: 637 32 77 32 197
Fragments arranged by size:
637 197 77 32 32
HindIII A'AGCT_T
Cuts at: 0 892 975
Size: 892 83
MfeI C'AATT_G
Cuts at: 0 417 975
Size: 417 558
MscI TGG'CCA
Cuts at: 0 727 829 975
Size: 727 102 146
Fragments arranged by size:
727 146 102
MslI CAynn'nnrTG
Cuts at: 0 619 701 736 875 877 975
Size: 619 82 35 139 2 98
Fragments arranged by size:
619 139 98 82 35 2
MspA1I CmG'CkG
Cuts at: 0 27 346 975
Size: 27 319 629
Fragments arranged by size:
629 319 27
NspI r_CATG'y
Cuts at: 0 186 651 852 975
Size: 186 465 201 123
Fragments arranged by size:
465 201 186 123
PasI CC'CwG_GG
Cuts at: 0 603 631 975
Size: 603 28 344
Fragments arranged by size:
603 344 28
PstI C_TGCA'G
Cuts at: 0 749 975
Size: 749 226
SacI G_AGCT'C
Cuts at: 0 398 975
Size: 398 577
SacII CC_GC'GG
Cuts at: 0 28 975
Size: 28 947
SapI GCTCTTCn'nnn_
Cuts at: 0 403 975
Size: 403 572
SexAI A'CCwGG_T
Cuts at: 0 839 975
Size: 839 136
SfcI C'TryA_G
Cuts at: 0 745 975
Size: 745 230
SspI AAT'ATT
Cuts at: 0 483 975
Size: 483 492
StyI C'CwwG_G
Cuts at: 0 362 975
Size: 362 613
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 975
Size: 244 731
Tth111I GACn'n_nGTC
Cuts at: 0 841 975
Size: 841 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 709 735 837 975
Size: 709 26 102 138
Fragments arranged by size:
709 138 102 26
XmnI GAAnn'nnTTC
Cuts at: 0 334 975
Size: 334 641
Enzymes that do cut:
AccI AcuI AhdI AleI ApoI AvaI BanI BanII
BbsI BbvCI BglII Bme1580I BmrI BpmI Bpu10I BsaHI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI
EaeI EarI EcoICRI Eco57MI EcoNI HaeII Hin4I HindIII
MfeI MscI MslI MspA1I NspI PasI PstI SacI
SacII SapI SexAI SfcI SspI StyI TaqII Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI XbaI XhoI
XmaI ZraI