Restriction map for
pFN21AE4314
(Linear) (Six-base) MAPSORT of: KIEE4314.seq Check: 759 from: 1 to: 969
KIEE4314.orf
With 182 enzymes: SgfI *
March 22, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 834 969
Size: 834 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 969
Size: 851 118
AflIII A'CryG_T
Cuts at: 0 156 969
Size: 156 813
AlwNI CAG_nnn'CTG
Cuts at: 0 361 969
Size: 361 608
ApoI r'AATT_y
Cuts at: 0 853 969
Size: 853 116
BamHI G'GATC_C
Cuts at: 0 658 969
Size: 658 311
BanI G'GyrC_C
Cuts at: 0 64 969
Size: 64 905
BanII G_rGCy'C
Cuts at: 0 545 969
Size: 545 424
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 969
Size: 236 733
BmrI ACTGGGnnnn_n'
Cuts at: 0 709 969
Size: 709 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 969
Size: 538 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 969
Size: 529 440
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 969
Size: 676 30 263
Fragments arranged by size:
676 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 886 969
Size: 187 699 83
Fragments arranged by size:
699 187 83
BseYI C'CCAG_C
Cuts at: 0 753 969
Size: 753 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 862 969
Size: 137 725 107
Fragments arranged by size:
725 137 107
BsiHKAI G_wGCw'C
Cuts at: 0 654 704 969
Size: 654 50 265
Fragments arranged by size:
654 265 50
Bsp1286I G_dGCh'C
Cuts at: 0 545 654 704 969
Size: 545 109 50 265
Fragments arranged by size:
545 265 109 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 834 969
Size: 168 666 135
Fragments arranged by size:
666 168 135
BstBI TT'CG_AA
Cuts at: 0 7 969
Size: 7 962
BstYI r'GATC_y
Cuts at: 0 426 658 969
Size: 426 232 311
Fragments arranged by size:
426 311 232
BtgI C'CryG_G
Cuts at: 0 449 676 969
Size: 449 227 293
Fragments arranged by size:
449 293 227
BtsI GCAGTG_nn'
Cuts at: 0 222 969
Size: 222 747
EaeI y'GGCC_r
Cuts at: 0 188 476 803 969
Size: 188 288 327 166
Fragments arranged by size:
327 288 188 166
EarI CTCTTCn'nnn_
Cuts at: 0 217 969
Size: 217 752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 851 969
Size: 538 313 118
Fragments arranged by size:
538 313 118
EcoNI CCTnn'n_nnAGG
Cuts at: 0 297 969
Size: 297 672
EcoO109I rG'GnC_Cy
Cuts at: 0 711 969
Size: 711 258
EcoRI G'AATT_C
Cuts at: 0 853 969
Size: 853 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 676 708 969
Size: 176 32 468 32 261
Fragments arranged by size:
468 261 176 32 32
HincII GTy'rAC
Cuts at: 0 333 863 969
Size: 333 530 106
Fragments arranged by size:
530 333 106
HindIII A'AGCT_T
Cuts at: 0 250 969
Size: 250 719
MfeI C'AATT_G
Cuts at: 0 319 969
Size: 319 650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 309 521 969
Size: 162 147 212 448
Fragments arranged by size:
448 212 162 147
MscI TGG'CCA
Cuts at: 0 190 478 805 969
Size: 190 288 327 164
Fragments arranged by size:
327 288 190 164
MslI CAynn'nnrTG
Cuts at: 0 490 969
Size: 490 479
MspA1I CmG'CkG
Cuts at: 0 780 969
Size: 780 189
NcoI C'CATG_G
Cuts at: 0 449 676 969
Size: 449 227 293
Fragments arranged by size:
449 293 227
NspI r_CATG'y
Cuts at: 0 160 969
Size: 160 809
PciI A'CATG_T
Cuts at: 0 156 969
Size: 156 813
PpuMI rG'GwC_Cy
Cuts at: 0 711 969
Size: 711 258
PstI C_TGCA'G
Cuts at: 0 81 785 969
Size: 81 704 184
Fragments arranged by size:
704 184 81
PvuII CAG'CTG
Cuts at: 0 780 969
Size: 780 189
SexAI A'CCwGG_T
Cuts at: 0 566 969
Size: 566 403
SfcI C'TryA_G
Cuts at: 0 77 645 781 969
Size: 77 568 136 188
Fragments arranged by size:
568 188 136 77
SmlI C'TyrA_G
Cuts at: 0 544 969
Size: 544 425
StyI C'CwwG_G
Cuts at: 0 293 449 676 728 969
Size: 293 156 227 52 241
Fragments arranged by size:
293 241 227 156 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 969
Size: 669 300
TatI w'GTAC_w
Cuts at: 0 548 969
Size: 548 421
Tth111I GACn'n_nGTC
Cuts at: 0 715 969
Size: 715 254
XbaI T'CTAG_A
Cuts at: 0 102 969
Size: 102 867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 784 969
Size: 784 185
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI BamHI BanI BanII
BbsI BmrI BpmI BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BstBI BstYI BtgI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NspI PciI
PpuMI PstI PvuII SexAI SfcI SmlI StyI TaqII
TatI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XhoI XmaI XmnI ZraI