Restriction map for
pFN21AE4313
(Linear) (Six-base) MAPSORT of: KIEE4313.seq Check: 321 from: 1 to: 966
KIEE4313.orf
With 182 enzymes: SgfI *
March 22, 2012 17:42 ..
AccI GT'mk_AC
Cuts at: 0 637 966
Size: 637 329
AleI CACnn'nnGTG
Cuts at: 0 127 966
Size: 127 839
AlwNI CAG_nnn'CTG
Cuts at: 0 153 646 966
Size: 153 493 320
Fragments arranged by size:
493 320 153
ApoI r'AATT_y
Cuts at: 0 156 481 966
Size: 156 325 485
Fragments arranged by size:
485 325 156
BmtI G_CTAG'C
Cuts at: 0 242 966
Size: 242 724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 966
Size: 545 421
Bpu10I CC'TnA_GC
Cuts at: 0 170 966
Size: 170 796
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 534 537 564 567 966
Size: 534 3 27 3 399
Fragments arranged by size:
534 399 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 542 724 868 966
Size: 542 182 144 98
Fragments arranged by size:
542 182 144 98
BseYI C'CCAG_C
Cuts at: 0 189 263 691 966
Size: 189 74 428 275
Fragments arranged by size:
428 275 189 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 966
Size: 98 868
BsmI GAATG_Cn'
Cuts at: 0 416 673 966
Size: 416 257 293
Fragments arranged by size:
416 293 257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 966
Size: 543 423
BsrFI r'CCGG_y
Cuts at: 0 205 966
Size: 205 761
BssSI C'ACGA_G
Cuts at: 0 48 411 966
Size: 48 363 555
Fragments arranged by size:
555 363 48
BstYI r'GATC_y
Cuts at: 0 441 966
Size: 441 525
BtgI C'CryG_G
Cuts at: 0 580 710 966
Size: 580 130 256
Fragments arranged by size:
580 256 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 960 966
Size: 960 6
EaeI y'GGCC_r
Cuts at: 0 2 203 966
Size: 2 201 763
Fragments arranged by size:
763 201 2
EarI CTCTTCn'nnn_
Cuts at: 0 77 742 908 966
Size: 77 665 166 58
Fragments arranged by size:
665 166 77 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 966
Size: 545 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 966
Size: 269 32 665
Fragments arranged by size:
665 269 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 966
Size: 249 717
MscI TGG'CCA
Cuts at: 0 4 966
Size: 4 962
MslI CAynn'nnrTG
Cuts at: 0 97 127 966
Size: 97 30 839
Fragments arranged by size:
839 97 30
MspA1I CmG'CkG
Cuts at: 0 153 754 966
Size: 153 601 212
Fragments arranged by size:
601 212 153
NaeI GCC'GGC
Cuts at: 0 207 966
Size: 207 759
NgoMIV G'CCGG_C
Cuts at: 0 205 966
Size: 205 761
NheI G'CTAG_C
Cuts at: 0 238 966
Size: 238 728
PstI C_TGCA'G
Cuts at: 0 753 966
Size: 753 213
PvuII CAG'CTG
Cuts at: 0 153 754 966
Size: 153 601 212
Fragments arranged by size:
601 212 153
SapI GCTCTTCn'nnn_
Cuts at: 0 77 966
Size: 77 889
SfcI C'TryA_G
Cuts at: 0 180 638 749 966
Size: 180 458 111 217
Fragments arranged by size:
458 217 180 111
StuI AGG'CCT
Cuts at: 0 178 793 966
Size: 178 615 173
Fragments arranged by size:
615 178 173
StyI C'CwwG_G
Cuts at: 0 63 966
Size: 63 903
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 966
Size: 322 644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 561 593 966
Size: 426 32 103 32 373
Fragments arranged by size:
426 373 103 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 966
Size: 54 912
XmnI GAAnn'nnTTC
Cuts at: 0 793 966
Size: 793 173
Enzymes that do cut:
AccI AleI AlwNI ApoI BmtI BpmI Bpu10I BsaXI
BseRI BseYI BsgI BsmI BspMI BsrFI BssSI BstYI
BtgI BtgZI EaeI EarI Eco57MI Hin4I MmeI MscI
MslI MspA1I NaeI NgoMIV NheI PstI PvuII SapI
SfcI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI ZraI