(Linear) (Six-base) MAPSORT of: KIEE4312.seq  Check: 5916  from: 1  to: 966



KIEE4312.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:42  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     966 
   Size:        533     433
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     744     966 
   Size:        744     222
 
ApoI r'AATT_y

Cuts at:      0      90     262     626     966 
   Size:         90     172     364     340

  Fragments arranged by size: 

                 364     340     172      90
 
BclI T'GATC_A

Cuts at:      0     308     966 
   Size:        308     658
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     331     456     832     966 
   Size:        331     125     376     134

  Fragments arranged by size: 

                 376     331     134     125
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     966 
   Size:        101     865
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     335     881     966 
   Size:        335     546      85

  Fragments arranged by size: 

                 546     335      85
 
BsrGI T'GTAC_A

Cuts at:      0     591     966 
   Size:        591     375
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     621     966 
   Size:        621     345
 
BstYI r'GATC_y

Cuts at:      0     152     966 
   Size:        152     814
 
BtgI C'CryG_G

Cuts at:      0      13     966 
   Size:         13     953
 
BtsI GCAGTG_nn'

Cuts at:      0     173     966 
   Size:        173     793
 
EaeI y'GGCC_r

Cuts at:      0     575     966 
   Size:        575     391
 
EarI CTCTTCn'nnn_

Cuts at:      0      12     966 
   Size:         12     954
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     533     966 
   Size:        101     432     433

  Fragments arranged by size: 

                 433     432     101
 
EcoRI G'AATT_C

Cuts at:      0      90     966 
   Size:         90     876
 
HaeII r_GCGC'y

Cuts at:      0     944     966 
   Size:        944      22
 
HincII GTy'rAC

Cuts at:      0     300     966 
   Size:        300     666
 
MscI TGG'CCA

Cuts at:      0     577     966 
   Size:        577     389
 
NdeI CA'TA_TG

Cuts at:      0     377     879     966 
   Size:        377     502      87

  Fragments arranged by size: 

                 502     377      87
 
NspI r_CATG'y

Cuts at:      0     182     298     966 
   Size:        182     116     668

  Fragments arranged by size: 

                 668     182     116
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     448     480     966 
   Size:        448      32     486

  Fragments arranged by size: 

                 486     448      32
 
PstI C_TGCA'G

Cuts at:      0     564     966 
   Size:        564     402
 
SfcI C'TryA_G

Cuts at:      0     560     944     966 
   Size:        560     384      22

  Fragments arranged by size: 

                 560     384      22
 
StyI C'CwwG_G

Cuts at:      0     252     966 
   Size:        252     714
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     350     947     966 
   Size:        350     597      19

  Fragments arranged by size: 

                 597     350      19
 
TatI w'GTAC_w

Cuts at:      0     591     966 
   Size:        591     375
 
Tth111I GACn'n_nGTC

Cuts at:      0     830     966 
   Size:        830     136
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     181     966 
   Size:        181     785
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BclI     BmrI     BpmI    BseRI    BsrGI
    BstXI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoRI
    HaeII   HincII     MscI     NdeI     NspI     PsrI     PstI     SfcI
     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI