Restriction map for
pFN21AE4311
(Linear) (Six-base) MAPSORT of: KIEE4311.seq Check: 9316 from: 1 to: 966
KIEE4311.orf
With 182 enzymes: SgfI *
March 22, 2012 17:41 ..
Acc65I G'GTAC_C
Cuts at: 0 71 966
Size: 71 895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 102 151 349 667 966
Size: 4 98 49 198 318 299
Fragments arranged by size:
318 299 198 98 49 4
AflIII A'CryG_T
Cuts at: 0 669 867 966
Size: 669 198 99
Fragments arranged by size:
669 198 99
AleI CACnn'nnGTG
Cuts at: 0 841 966
Size: 841 125
ApoI r'AATT_y
Cuts at: 0 946 966
Size: 946 20
AvaI C'yCGr_G
Cuts at: 0 265 966
Size: 265 701
BanI G'GyrC_C
Cuts at: 0 71 176 966
Size: 71 105 790
Fragments arranged by size:
790 105 71
BanII G_rGCy'C
Cuts at: 0 266 966
Size: 266 700
BbsI GAAGACnn'nnnn_
Cuts at: 0 673 966
Size: 673 293
BbvCI CC'TCA_GC
Cuts at: 0 82 396 699 966
Size: 82 314 303 267
Fragments arranged by size:
314 303 267 82
BclI T'GATC_A
Cuts at: 0 641 966
Size: 641 325
BglI GCCn_nnn'nGGC
Cuts at: 0 200 966
Size: 200 766
BglII A'GATC_T
Cuts at: 0 451 966
Size: 451 515
BlpI GC'TnA_GC
Cuts at: 0 190 401 966
Size: 190 211 565
Fragments arranged by size:
565 211 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 365 966
Size: 365 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 840 922 966
Size: 181 659 82 44
Fragments arranged by size:
659 181 82 44
Bpu10I CC'TnA_GC
Cuts at: 0 82 396 699 966
Size: 82 314 303 267
Fragments arranged by size:
314 303 267 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 966
Size: 337 629
BsaI GGTCTCn'nnnn_
Cuts at: 0 303 858 966
Size: 303 555 108
Fragments arranged by size:
555 303 108
BsaAI yAC'GTr
Cuts at: 0 672 870 966
Size: 672 198 96
Fragments arranged by size:
672 198 96
BsaHI Gr'CG_yC
Cuts at: 0 772 966
Size: 772 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 283 313 713 743 966
Size: 142 30 111 30 400 30 223
Fragments arranged by size:
400 223 142 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 456 966
Size: 283 173 510
Fragments arranged by size:
510 283 173
BseYI C'CCAG_C
Cuts at: 0 145 738 966
Size: 145 593 228
Fragments arranged by size:
593 228 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 966
Size: 220 746
BsiEI CG_ry'CG
Cuts at: 0 225 229 966
Size: 225 4 737
Fragments arranged by size:
737 225 4
BsiWI C'GTAC_G
Cuts at: 0 908 966
Size: 908 58
Bsp1286I G_dGCh'C
Cuts at: 0 266 966
Size: 266 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 694 966
Size: 694 272
BsrBI CCG'CTC
Cuts at: 0 405 966
Size: 405 561
BsrDI GCAATG_nn'
Cuts at: 0 555 966
Size: 555 411
BsrFI r'CCGG_y
Cuts at: 0 68 224 966
Size: 68 156 742
Fragments arranged by size:
742 156 68
BstYI r'GATC_y
Cuts at: 0 451 966
Size: 451 515
BtsI GCAGTG_nn'
Cuts at: 0 237 966
Size: 237 729
EaeI y'GGCC_r
Cuts at: 0 226 467 966
Size: 226 241 499
Fragments arranged by size:
499 241 226
EagI C'GGCC_G
Cuts at: 0 226 966
Size: 226 740
EarI CTCTTCn'nnn_
Cuts at: 0 121 966
Size: 121 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 966
Size: 109 857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 102 151 181 349 667 840 922
Size: 4 98 49 30 168 318 173 82
Cuts at: 922 966
Size: 44
Fragments arranged by size:
318 173 168 98 82 49 44 30
4
EcoRI G'AATT_C
Cuts at: 0 946 966
Size: 946 20
FspI TGC'GCA
Cuts at: 0 243 847 966
Size: 243 604 119
Fragments arranged by size:
604 243 119
FspAI rTGC'GCAy
Cuts at: 0 847 966
Size: 847 119
HaeII r_GCGC'y
Cuts at: 0 125 966
Size: 125 841
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 966
Size: 283 32 651
Fragments arranged by size:
651 283 32
KpnI G_GTAC'C
Cuts at: 0 75 966
Size: 75 891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 966
Size: 325 641
MscI TGG'CCA
Cuts at: 0 469 966
Size: 469 497
MslI CAynn'nnrTG
Cuts at: 0 674 841 966
Size: 674 167 125
Fragments arranged by size:
674 167 125
MspA1I CmG'CkG
Cuts at: 0 234 400 900 966
Size: 234 166 500 66
Fragments arranged by size:
500 234 166 66
NspI r_CATG'y
Cuts at: 0 189 966
Size: 189 777
PasI CC'CwG_GG
Cuts at: 0 663 966
Size: 663 303
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 502 966
Size: 465 37 464
Fragments arranged by size:
465 464 37
PstI C_TGCA'G
Cuts at: 0 616 966
Size: 616 350
PvuII CAG'CTG
Cuts at: 0 234 400 900 966
Size: 234 166 500 66
Fragments arranged by size:
500 234 166 66
SapI GCTCTTCn'nnn_
Cuts at: 0 121 966
Size: 121 845
SexAI A'CCwGG_T
Cuts at: 0 293 966
Size: 293 673
SfcI C'TryA_G
Cuts at: 0 612 966
Size: 612 354
SmlI C'TyrA_G
Cuts at: 0 352 966
Size: 352 614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 713 745 966
Size: 713 32 221
Fragments arranged by size:
713 221 32
Tth111I GACn'n_nGTC
Cuts at: 0 295 966
Size: 295 671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 820 966
Size: 820 146
Enzymes that do cut:
Acc65I AcuI AflIII AleI ApoI AvaI BanI BanII
BbsI BbvCI BclI BglI BglII BlpI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiWI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstYI BtsI EaeI EagI EarI EciI Eco57MI EcoRI
FspI FspAI HaeII Hin4I KpnI MmeI MscI MslI
MspA1I NspI PasI PflMI PstI PvuII SapI SexAI
SfcI SmlI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI Bme1580I
BmgBI BmtI BplI BsaBI BsaWI BsiHKAI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
HincII HindIII HpaI KasI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI XbaI XhoI XmaI XmnI ZraI