Restriction map for
pFN21AE4310
(Linear) (Six-base) MAPSORT of: KIEE4310.seq Check: 8571 from: 1 to: 963
KIEE4310.orf
With 182 enzymes: SgfI *
March 22, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 963
Size: 228 735
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 229 963
Size: 23 206 734
Fragments arranged by size:
734 206 23
AflIII A'CryG_T
Cuts at: 0 842 963
Size: 842 121
AhdI GACnn_n'nnGTC
Cuts at: 0 210 963
Size: 210 753
AlwNI CAG_nnn'CTG
Cuts at: 0 72 537 963
Size: 72 465 426
Fragments arranged by size:
465 426 72
ApoI r'AATT_y
Cuts at: 0 580 963
Size: 580 383
AvaI C'yCGr_G
Cuts at: 0 827 963
Size: 827 136
BanI G'GyrC_C
Cuts at: 0 97 790 963
Size: 97 693 173
Fragments arranged by size:
693 173 97
BanII G_rGCy'C
Cuts at: 0 95 963
Size: 95 868
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 963
Size: 113 850
Bme1580I G_kGCm'C
Cuts at: 0 793 861 963
Size: 793 68 102
Fragments arranged by size:
793 102 68
BmgBI CAC'GTC
Cuts at: 0 279 963
Size: 279 684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 938 963
Size: 569 369 25
Fragments arranged by size:
569 369 25
BsaHI Gr'CG_yC
Cuts at: 0 110 259 963
Size: 110 149 704
Fragments arranged by size:
704 149 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 11 41 963
Size: 11 30 922
Fragments arranged by size:
922 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 963
Size: 376 587
BseYI C'CCAG_C
Cuts at: 0 146 963
Size: 146 817
BsiHKAI G_wGCw'C
Cuts at: 0 181 963
Size: 181 782
Bsp1286I G_dGCh'C
Cuts at: 0 95 181 793 861 963
Size: 95 86 612 68 102
Fragments arranged by size:
612 102 95 86 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 963
Size: 228 735
BsrFI r'CCGG_y
Cuts at: 0 310 963
Size: 310 653
BssSI C'ACGA_G
Cuts at: 0 514 963
Size: 514 449
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 754 963
Size: 288 466 209
Fragments arranged by size:
466 288 209
BstYI r'GATC_y
Cuts at: 0 338 365 963
Size: 338 27 598
Fragments arranged by size:
598 338 27
Bsu36I CC'TnA_GG
Cuts at: 0 73 359 963
Size: 73 286 604
Fragments arranged by size:
604 286 73
BtgI C'CryG_G
Cuts at: 0 5 53 263 963
Size: 5 48 210 700
Fragments arranged by size:
700 210 48 5
ClaI AT'CG_AT
Cuts at: 0 775 963
Size: 775 188
EaeI y'GGCC_r
Cuts at: 0 2 963
Size: 2 961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 229 569 938 963
Size: 23 206 340 369 25
Fragments arranged by size:
369 340 206 25 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 76 541 963
Size: 76 465 422
Fragments arranged by size:
465 422 76
EcoO109I rG'GnC_Cy
Cuts at: 0 534 963
Size: 534 429
EcoRV GAT'ATC
Cuts at: 0 650 852 963
Size: 650 202 111
Fragments arranged by size:
650 202 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 955 963
Size: 955 8
HaeII r_GCGC'y
Cuts at: 0 153 963
Size: 153 810
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 196 228 412 444 810 842
Size: 11 32 153 32 184 32 366 32
Cuts at: 842 963
Size: 121
Fragments arranged by size:
366 184 153 121 32 32 32 32
11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 963
Size: 827 136
MscI TGG'CCA
Cuts at: 0 4 963
Size: 4 959
MslI CAynn'nnrTG
Cuts at: 0 814 963
Size: 814 149
MspA1I CmG'CkG
Cuts at: 0 475 963
Size: 475 488
NaeI GCC'GGC
Cuts at: 0 312 963
Size: 312 651
NcoI C'CATG_G
Cuts at: 0 5 53 963
Size: 5 48 910
Fragments arranged by size:
910 48 5
NgoMIV G'CCGG_C
Cuts at: 0 310 963
Size: 310 653
NspI r_CATG'y
Cuts at: 0 628 846 963
Size: 628 218 117
Fragments arranged by size:
628 218 117
PciI A'CATG_T
Cuts at: 0 842 963
Size: 842 121
PfoI T'CCnGG_A
Cuts at: 0 349 601 963
Size: 349 252 362
Fragments arranged by size:
362 349 252
PpuMI rG'GwC_Cy
Cuts at: 0 534 963
Size: 534 429
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 926 958 963
Size: 926 32 5
Fragments arranged by size:
926 32 5
PvuII CAG'CTG
Cuts at: 0 475 963
Size: 475 488
ScaI AGT'ACT
Cuts at: 0 935 963
Size: 935 28
SfcI C'TryA_G
Cuts at: 0 719 924 963
Size: 719 205 39
Fragments arranged by size:
719 205 39
SmlI C'TyrA_G
Cuts at: 0 827 963
Size: 827 136
StyI C'CwwG_G
Cuts at: 0 5 53 963
Size: 5 48 910
Fragments arranged by size:
910 48 5
TatI w'GTAC_w
Cuts at: 0 890 933 963
Size: 890 43 30
Fragments arranged by size:
890 43 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 963
Size: 397 566
XhoI C'TCGA_G
Cuts at: 0 827 963
Size: 827 136
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApoI AvaI BanI
BanII BbsI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstYI
Bsu36I BtgI ClaI EaeI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PfoI PpuMI PsrI PvuII
ScaI SfcI SmlI StyI TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI
BstEII BstXI BstZ17I BtgZI BtsI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoRI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XmaI XmnI ZraI