Restriction map for
pFN21AE4308
(Linear) (Six-base) MAPSORT of: KIEE4308.seq Check: 2999 from: 1 to: 954
KIEE4308.orf
With 182 enzymes: SgfI *
March 22, 2012 17:41 ..
AccI GT'mk_AC
Cuts at: 0 950 954
Size: 950 4
AlwNI CAG_nnn'CTG
Cuts at: 0 69 954
Size: 69 885
ApoI r'AATT_y
Cuts at: 0 535 954
Size: 535 419
AvaI C'yCGr_G
Cuts at: 0 374 461 954
Size: 374 87 493
Fragments arranged by size:
493 374 87
BanI G'GyrC_C
Cuts at: 0 22 122 954
Size: 22 100 832
Fragments arranged by size:
832 100 22
BbeI G_GCGC'C
Cuts at: 0 126 954
Size: 126 828
BbsI GAAGACnn'nnnn_
Cuts at: 0 84 954
Size: 84 870
BciVI GTATCCnnnnn_n'
Cuts at: 0 584 954
Size: 584 370
BclI T'GATC_A
Cuts at: 0 473 954
Size: 473 481
BglI GCCn_nnn'nGGC
Cuts at: 0 676 700 954
Size: 676 24 254
Fragments arranged by size:
676 254 24
Bme1580I G_kGCm'C
Cuts at: 0 681 954
Size: 681 273
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 954
Size: 570 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 664 673 823 954
Size: 565 99 9 150 131
Fragments arranged by size:
565 150 131 99 9
Bpu10I CC'TnA_GC
Cuts at: 0 52 954
Size: 52 902
BsaBI GATnn'nnATC
Cuts at: 0 478 954
Size: 478 476
BsaHI Gr'CG_yC
Cuts at: 0 123 954
Size: 123 831
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 954
Size: 514 30 410
Fragments arranged by size:
514 410 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 954
Size: 207 747
BseYI C'CCAG_C
Cuts at: 0 18 674 954
Size: 18 656 280
Fragments arranged by size:
656 280 18
BsiHKAI G_wGCw'C
Cuts at: 0 917 954
Size: 917 37
Bsp1286I G_dGCh'C
Cuts at: 0 681 917 954
Size: 681 236 37
Fragments arranged by size:
681 236 37
BspHI T'CATG_A
Cuts at: 0 755 954
Size: 755 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 896 954
Size: 209 687 58
Fragments arranged by size:
687 209 58
BsrDI GCAATG_nn'
Cuts at: 0 87 954
Size: 87 867
BsrFI r'CCGG_y
Cuts at: 0 59 100 954
Size: 59 41 854
Fragments arranged by size:
854 59 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 954
Size: 69 885
BstEII G'GTnAC_C
Cuts at: 0 426 954
Size: 426 528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 285 492 903 954
Size: 240 45 207 411 51
Fragments arranged by size:
411 240 207 51 45
Bsu36I CC'TnA_GG
Cuts at: 0 565 954
Size: 565 389
BtgI C'CryG_G
Cuts at: 0 364 954
Size: 364 590
BtsI GCAGTG_nn'
Cuts at: 0 44 133 954
Size: 44 89 821
Fragments arranged by size:
821 89 44
EaeI y'GGCC_r
Cuts at: 0 167 954
Size: 167 787
EarI CTCTTCn'nnn_
Cuts at: 0 17 954
Size: 17 937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 664 673 823 954
Size: 565 99 9 150 131
Fragments arranged by size:
565 150 131 99 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 954
Size: 784 170
EcoO109I rG'GnC_Cy
Cuts at: 0 198 415 716 924 954
Size: 198 217 301 208 30
Fragments arranged by size:
301 217 208 198 30
EcoRV GAT'ATC
Cuts at: 0 246 954
Size: 246 708
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 328 954
Size: 328 626
HaeII r_GCGC'y
Cuts at: 0 126 954
Size: 126 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 587 619 954
Size: 587 32 335
Fragments arranged by size:
587 335 32
KasI G'GCGC_C
Cuts at: 0 122 954
Size: 122 832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 954
Size: 221 733
MscI TGG'CCA
Cuts at: 0 169 954
Size: 169 785
MspA1I CmG'CkG
Cuts at: 0 141 173 207 390 954
Size: 141 32 34 183 564
Fragments arranged by size:
564 183 141 34 32
NarI GG'CG_CC
Cuts at: 0 123 954
Size: 123 831
NcoI C'CATG_G
Cuts at: 0 364 954
Size: 364 590
NspI r_CATG'y
Cuts at: 0 811 954
Size: 811 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 365 954
Size: 365 589
PpuMI rG'GwC_Cy
Cuts at: 0 198 415 924 954
Size: 198 217 509 30
Fragments arranged by size:
509 217 198 30
PstI C_TGCA'G
Cuts at: 0 140 206 455 668 954
Size: 140 66 249 213 286
Fragments arranged by size:
286 249 213 140 66
PvuII CAG'CTG
Cuts at: 0 141 173 954
Size: 141 32 781
Fragments arranged by size:
781 141 32
SanDI GG'GwC_CC
Cuts at: 0 415 954
Size: 415 539
SbfI CC_TGCA'GG
Cuts at: 0 668 954
Size: 668 286
SfcI C'TryA_G
Cuts at: 0 136 202 451 664 840 954
Size: 136 66 249 213 176 114
Fragments arranged by size:
249 213 176 136 114 66
SfoI GGC'GCC
Cuts at: 0 124 954
Size: 124 830
SmaI CCC'GGG
Cuts at: 0 376 463 954
Size: 376 87 491
Fragments arranged by size:
491 376 87
SphI G_CATG'C
Cuts at: 0 811 954
Size: 811 143
StuI AGG'CCT
Cuts at: 0 550 670 954
Size: 550 120 284
Fragments arranged by size:
550 284 120
StyI C'CwwG_G
Cuts at: 0 364 785 954
Size: 364 421 169
Fragments arranged by size:
421 364 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 10 273 954
Size: 10 263 681
Fragments arranged by size:
681 263 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 431 954
Size: 399 32 523
Fragments arranged by size:
523 399 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 954
Size: 540 414
XmaI C'CCGG_G
Cuts at: 0 374 461 954
Size: 374 87 493
Fragments arranged by size:
493 374 87
Enzymes that do cut:
AccI AlwNI ApoI AvaI BanI BbeI BbsI BciVI
BclI BglI Bme1580I BplI BpmI Bpu10I BsaBI BsaHI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BstAPI BstEII BstXI Bsu36I BtgI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
KasI MmeI MscI MspA1I NarI NcoI NspI PflMI
PpuMI PstI PvuII SanDI SbfI SfcI SfoI SmaI
SphI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI
BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI
BsaAI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI