Restriction map for
pFN21AE4307
(Linear) (Six-base) MAPSORT of: KIEE4307.seq Check: 8047 from: 1 to: 936
KIEE4307.orf
With 182 enzymes: SgfI *
March 22, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 353 936
Size: 353 583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 936
Size: 497 439
AhdI GACnn_n'nnGTC
Cuts at: 0 150 936
Size: 150 786
AleI CACnn'nnGTG
Cuts at: 0 66 936
Size: 66 870
AlwNI CAG_nnn'CTG
Cuts at: 0 546 747 765 825 936
Size: 546 201 18 60 111
Fragments arranged by size:
546 201 111 60 18
ApaI G_GGCC'C
Cuts at: 0 858 882 901 936
Size: 858 24 19 35
Fragments arranged by size:
858 35 24 19
AvaI C'yCGr_G
Cuts at: 0 858 936
Size: 858 78
BanI G'GyrC_C
Cuts at: 0 111 936
Size: 111 825
BanII G_rGCy'C
Cuts at: 0 376 667 722 858 882 901 936
Size: 376 291 55 136 24 19 35
Fragments arranged by size:
376 291 136 55 35 24 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 936
Size: 485 451
Bme1580I G_kGCm'C
Cuts at: 0 263 858 882 901 936
Size: 263 595 24 19 35
Fragments arranged by size:
595 263 35 24 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 936
Size: 208 728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 323 936
Size: 301 22 613
Fragments arranged by size:
613 301 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 936
Size: 544 30 362
Fragments arranged by size:
544 362 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 320 646 936
Size: 320 326 290
Fragments arranged by size:
326 320 290
BseYI C'CCAG_C
Cuts at: 0 825 894 936
Size: 825 69 42
Fragments arranged by size:
825 69 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 331 936
Size: 74 257 605
Fragments arranged by size:
605 257 74
BsiEI CG_ry'CG
Cuts at: 0 868 936
Size: 868 68
BsiHKAI G_wGCw'C
Cuts at: 0 383 401 589 936
Size: 383 18 188 347
Fragments arranged by size:
383 347 188 18
BsmI GAATG_Cn'
Cuts at: 0 751 936
Size: 751 185
Bsp1286I G_dGCh'C
Cuts at: 0 263 376 383 401 589 667 722 858
Size: 263 113 7 18 188 78 55 136
Cuts at: 858 882 901 936
Size: 24 19 35
Fragments arranged by size:
263 188 136 113 78 55 35 24
19 18 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 626 691 936
Size: 353 273 65 245
Fragments arranged by size:
353 273 245 65
BsrFI r'CCGG_y
Cuts at: 0 922 936
Size: 922 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 122 936
Size: 122 814
BstYI r'GATC_y
Cuts at: 0 743 936
Size: 743 193
BtgI C'CryG_G
Cuts at: 0 32 407 936
Size: 32 375 529
Fragments arranged by size:
529 375 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 701 936
Size: 701 235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 108 143 936
Size: 108 35 793
Fragments arranged by size:
793 108 35
DraIII CAC_nnn'GTG
Cuts at: 0 267 936
Size: 267 669
EaeI y'GGCC_r
Cuts at: 0 40 47 865 920 936
Size: 40 7 818 55 16
Fragments arranged by size:
818 55 40 16 7
EagI C'GGCC_G
Cuts at: 0 865 936
Size: 865 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 323 497 936
Size: 301 22 174 439
Fragments arranged by size:
439 301 174 22
EcoO109I rG'GnC_Cy
Cuts at: 0 471 812 854 855 879 898 936
Size: 471 341 42 1 24 19 38
Fragments arranged by size:
471 341 42 38 24 19 1
HaeII r_GCGC'y
Cuts at: 0 19 936
Size: 19 917
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 544 576 728 760 936
Size: 136 32 376 32 152 32 176
Fragments arranged by size:
376 176 152 136 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 380 593 936
Size: 119 261 213 343
Fragments arranged by size:
343 261 213 119
MslI CAynn'nnrTG
Cuts at: 0 66 412 592 936
Size: 66 346 180 344
Fragments arranged by size:
346 344 180 66
MspA1I CmG'CkG
Cuts at: 0 34 825 870 936
Size: 34 791 45 66
Fragments arranged by size:
791 66 45 34
NaeI GCC'GGC
Cuts at: 0 924 936
Size: 924 12
NcoI C'CATG_G
Cuts at: 0 407 936
Size: 407 529
NgoMIV G'CCGG_C
Cuts at: 0 922 936
Size: 922 14
PasI CC'CwG_GG
Cuts at: 0 875 936
Size: 875 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 546 936
Size: 546 390
PfoI T'CCnGG_A
Cuts at: 0 713 936
Size: 713 223
PpuMI rG'GwC_Cy
Cuts at: 0 471 936
Size: 471 465
PspOMI G'GGCC_C
Cuts at: 0 854 878 897 936
Size: 854 24 19 39
Fragments arranged by size:
854 39 24 19
PstI C_TGCA'G
Cuts at: 0 821 936
Size: 821 115
PvuII CAG'CTG
Cuts at: 0 825 936
Size: 825 111
SacII CC_GC'GG
Cuts at: 0 35 936
Size: 35 901
SfcI C'TryA_G
Cuts at: 0 817 936
Size: 817 119
SmaI CCC'GGG
Cuts at: 0 860 936
Size: 860 76
StyI C'CwwG_G
Cuts at: 0 176 407 626 936
Size: 176 231 219 310
Fragments arranged by size:
310 231 219 176
TatI w'GTAC_w
Cuts at: 0 423 767 936
Size: 423 344 169
Fragments arranged by size:
423 344 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 936
Size: 509 32 395
Fragments arranged by size:
509 395 32
Tth111I GACn'n_nGTC
Cuts at: 0 805 936
Size: 805 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 936
Size: 183 753
XmaI C'CCGG_G
Cuts at: 0 858 936
Size: 858 78
Enzymes that do cut:
AarI AcuI AhdI AleI AlwNI ApaI AvaI BanI
BanII BbsI Bme1580I BmrI BpmI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstXI
BstYI BtgI BtgZI CspCI DraIII EaeI EagI Eco57MI
EcoO109I HaeII Hin4I MmeI MslI MspA1I NaeI NcoI
NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacII SfcI SmaI StyI TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI DraI DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XbaI
XhoI XmnI ZraI