Restriction map for
pFN21AE4306
(Linear) (Six-base) MAPSORT of: KIEE4306.seq Check: 2118 from: 1 to: 918
KIEE4306.orf
With 182 enzymes: SgfI *
March 22, 2012 17:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 853 899 918
Size: 685 168 46 19
Fragments arranged by size:
685 168 46 19
AflII C'TTAA_G
Cuts at: 0 144 918
Size: 144 774
AleI CACnn'nnGTG
Cuts at: 0 476 918
Size: 476 442
AlwNI CAG_nnn'CTG
Cuts at: 0 78 918
Size: 78 840
ApoI r'AATT_y
Cuts at: 0 191 685 918
Size: 191 494 233
Fragments arranged by size:
494 233 191
AvaI C'yCGr_G
Cuts at: 0 62 918
Size: 62 856
BamHI G'GATC_C
Cuts at: 0 303 918
Size: 303 615
BanI G'GyrC_C
Cuts at: 0 90 918
Size: 90 828
BanII G_rGCy'C
Cuts at: 0 469 918
Size: 469 449
BbeI G_GCGC'C
Cuts at: 0 94 918
Size: 94 824
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 918
Size: 226 692
BclI T'GATC_A
Cuts at: 0 398 918
Size: 398 520
BmgBI CAC'GTC
Cuts at: 0 237 918
Size: 237 681
Bpu10I CC'TnA_GC
Cuts at: 0 299 918
Size: 299 619
BsaI GGTCTCn'nnnn_
Cuts at: 0 772 918
Size: 772 146
BsaBI GATnn'nnATC
Cuts at: 0 581 614 918
Size: 581 33 304
Fragments arranged by size:
581 304 33
BsaHI Gr'CG_yC
Cuts at: 0 4 91 918
Size: 4 87 827
Fragments arranged by size:
827 87 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 918
Size: 565 30 323
Fragments arranged by size:
565 323 30
BseYI C'CCAG_C
Cuts at: 0 505 918
Size: 505 413
BsiHKAI G_wGCw'C
Cuts at: 0 469 918
Size: 469 449
BsmI GAATG_Cn'
Cuts at: 0 507 918
Size: 507 411
Bsp1286I G_dGCh'C
Cuts at: 0 469 918
Size: 469 449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 918
Size: 61 857
BsrBI CCG'CTC
Cuts at: 0 624 918
Size: 624 294
BsrFI r'CCGG_y
Cuts at: 0 87 918
Size: 87 831
BsrGI T'GTAC_A
Cuts at: 0 788 918
Size: 788 130
BssSI C'ACGA_G
Cuts at: 0 538 918
Size: 538 380
BstYI r'GATC_y
Cuts at: 0 303 918
Size: 303 615
BtgI C'CryG_G
Cuts at: 0 46 918
Size: 46 872
BtsI GCAGTG_nn'
Cuts at: 0 374 918
Size: 374 544
EaeI y'GGCC_r
Cuts at: 0 747 918
Size: 747 171
EcoICRI GAG'CTC
Cuts at: 0 467 918
Size: 467 451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 853 899 918
Size: 685 168 46 19
Fragments arranged by size:
685 168 46 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 229 918
Size: 229 689
HaeII r_GCGC'y
Cuts at: 0 70 94 918
Size: 70 24 824
Fragments arranged by size:
824 70 24
KasI G'GCGC_C
Cuts at: 0 90 918
Size: 90 828
MscI TGG'CCA
Cuts at: 0 749 918
Size: 749 169
MslI CAynn'nnrTG
Cuts at: 0 431 476 918
Size: 431 45 442
Fragments arranged by size:
442 431 45
MspA1I CmG'CkG
Cuts at: 0 96 131 918
Size: 96 35 787
Fragments arranged by size:
787 96 35
NaeI GCC'GGC
Cuts at: 0 89 918
Size: 89 829
NarI GG'CG_CC
Cuts at: 0 91 918
Size: 91 827
NcoI C'CATG_G
Cuts at: 0 46 918
Size: 46 872
NgoMIV G'CCGG_C
Cuts at: 0 87 918
Size: 87 831
PasI CC'CwG_GG
Cuts at: 0 345 918
Size: 345 573
PflMI CCAn_nnn'nTGG
Cuts at: 0 913 918
Size: 913 5
PstI C_TGCA'G
Cuts at: 0 74 918
Size: 74 844
PvuII CAG'CTG
Cuts at: 0 131 918
Size: 131 787
SacI G_AGCT'C
Cuts at: 0 469 918
Size: 469 449
SfcI C'TryA_G
Cuts at: 0 70 713 851 918
Size: 70 643 138 67
Fragments arranged by size:
643 138 70 67
SfoI GGC'GCC
Cuts at: 0 92 918
Size: 92 826
SgrAI Cr'CCGG_yG
Cuts at: 0 87 918
Size: 87 831
SmlI C'TyrA_G
Cuts at: 0 144 918
Size: 144 774
StyI C'CwwG_G
Cuts at: 0 46 755 912 918
Size: 46 709 157 6
Fragments arranged by size:
709 157 46 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 918
Size: 142 776
TatI w'GTAC_w
Cuts at: 0 788 918
Size: 788 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 918
Size: 824 32 62
Fragments arranged by size:
824 62 32
XmnI GAAnn'nnTTC
Cuts at: 0 247 764 918
Size: 247 517 154
Fragments arranged by size:
517 247 154
Enzymes that do cut:
AcuI AflII AleI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BclI BmgBI Bpu10I BsaI BsaBI
BsaHI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstYI BtgI BtsI EaeI EcoICRI
Eco57MI EcoNI HaeII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PflMI PstI PvuII SacI
SfcI SfoI SgrAI SmlI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaAI
BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI