Restriction map for
pFN21AE4304
(Linear) (Six-base) MAPSORT of: KIEE4304.seq Check: 40 from: 1 to: 903
KIEE4304.orf
With 182 enzymes: SgfI *
March 22, 2012 17:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 903
Size: 394 509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 214 903
Size: 214 689
AlwNI CAG_nnn'CTG
Cuts at: 0 245 492 903
Size: 245 247 411
Fragments arranged by size:
411 247 245
ApoI r'AATT_y
Cuts at: 0 364 705 770 903
Size: 364 341 65 133
Fragments arranged by size:
364 341 133 65
BamHI G'GATC_C
Cuts at: 0 332 903
Size: 332 571
BanII G_rGCy'C
Cuts at: 0 830 903
Size: 830 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 628 903
Size: 594 34 275
Fragments arranged by size:
594 275 34
Bme1580I G_kGCm'C
Cuts at: 0 149 903
Size: 149 754
BmrI ACTGGGnnnn_n'
Cuts at: 0 502 903
Size: 502 401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 903
Size: 605 298
Bpu10I CC'TnA_GC
Cuts at: 0 202 903
Size: 202 701
BsaBI GATnn'nnATC
Cuts at: 0 337 512 903
Size: 337 175 391
Fragments arranged by size:
391 337 175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 838 903
Size: 838 65
BseYI C'CCAG_C
Cuts at: 0 123 155 474 631 903
Size: 123 32 319 157 272
Fragments arranged by size:
319 272 157 123 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 903
Size: 392 511
BsiHKAI G_wGCw'C
Cuts at: 0 203 903
Size: 203 700
BsmI GAATG_Cn'
Cuts at: 0 102 903
Size: 102 801
Bsp1286I G_dGCh'C
Cuts at: 0 149 203 830 903
Size: 149 54 627 73
Fragments arranged by size:
627 149 73 54
BsrBI CCG'CTC
Cuts at: 0 82 903
Size: 82 821
BsrDI GCAATG_nn'
Cuts at: 0 902 903
Size: 902 1
BssSI C'ACGA_G
Cuts at: 0 142 903
Size: 142 761
BstEII G'GTnAC_C
Cuts at: 0 399 903
Size: 399 504
BstYI r'GATC_y
Cuts at: 0 317 332 623 903
Size: 317 15 291 280
Fragments arranged by size:
317 291 280 15
Bsu36I CC'TnA_GG
Cuts at: 0 741 903
Size: 741 162
BtsI GCAGTG_nn'
Cuts at: 0 431 903
Size: 431 472
EaeI y'GGCC_r
Cuts at: 0 536 563 725 903
Size: 536 27 162 178
Fragments arranged by size:
536 178 162 27
EarI CTCTTCn'nnn_
Cuts at: 0 797 903
Size: 797 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 605 903
Size: 394 211 298
Fragments arranged by size:
394 298 211
EcoNI CCTnn'n_nnAGG
Cuts at: 0 444 740 903
Size: 444 296 163
Fragments arranged by size:
444 296 163
EcoO109I rG'GnC_Cy
Cuts at: 0 59 903
Size: 59 844
EcoRI G'AATT_C
Cuts at: 0 770 903
Size: 770 133
HaeII r_GCGC'y
Cuts at: 0 662 903
Size: 662 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 578 608 610 640 903
Size: 71 32 475 30 2 30 263
Fragments arranged by size:
475 263 71 32 30 30 2
MscI TGG'CCA
Cuts at: 0 538 565 727 903
Size: 538 27 162 176
Fragments arranged by size:
538 176 162 27
PasI CC'CwG_GG
Cuts at: 0 133 903
Size: 133 770
PflMI CCAn_nnn'nTGG
Cuts at: 0 245 903
Size: 245 658
PpuMI rG'GwC_Cy
Cuts at: 0 59 903
Size: 59 844
PstI C_TGCA'G
Cuts at: 0 422 438 903
Size: 422 16 465
Fragments arranged by size:
465 422 16
SfcI C'TryA_G
Cuts at: 0 418 434 903
Size: 418 16 469
Fragments arranged by size:
469 418 16
StyI C'CwwG_G
Cuts at: 0 395 445 903
Size: 395 50 458
Fragments arranged by size:
458 395 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 903
Size: 79 824
Tth111I GACn'n_nGTC
Cuts at: 0 25 903
Size: 25 878
XbaI T'CTAG_A
Cuts at: 0 556 903
Size: 556 347
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BamHI BanII BcgI Bme1580I
BmrI BpmI Bpu10I BsaBI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrBI BsrDI BssSI BstEII BstYI Bsu36I
BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I MscI PasI PflMI PpuMI PstI SfcI StyI
TaqII Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI XcmI XhoI XmaI XmnI
ZraI