Restriction map for
pFN21AE4301
(Linear) (Six-base) MAPSORT of: KIEE4301.seq Check: 9594 from: 1 to: 891
KIEE4301.orf
With 182 enzymes: SgfI *
March 22, 2012 17:39 ..
AccI GT'mk_AC
Cuts at: 0 800 891
Size: 800 91
AhdI GACnn_n'nnGTC
Cuts at: 0 128 891
Size: 128 763
AlwNI CAG_nnn'CTG
Cuts at: 0 99 594 891
Size: 99 495 297
Fragments arranged by size:
495 297 99
ApoI r'AATT_y
Cuts at: 0 184 451 891
Size: 184 267 440
Fragments arranged by size:
440 267 184
AvaI C'yCGr_G
Cuts at: 0 826 891
Size: 826 65
BanI G'GyrC_C
Cuts at: 0 245 505 547 554 625 754 891
Size: 245 260 42 7 71 129 137
Fragments arranged by size:
260 245 137 129 71 42 7
BanII G_rGCy'C
Cuts at: 0 851 891
Size: 851 40
BbeI G_GCGC'C
Cuts at: 0 558 758 891
Size: 558 200 133
Fragments arranged by size:
558 200 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 400 891
Size: 400 491
BglI GCCn_nnn'nGGC
Cuts at: 0 388 530 646 650 891
Size: 388 142 116 4 241
Fragments arranged by size:
388 241 142 116 4
BglII A'GATC_T
Cuts at: 0 680 891
Size: 680 211
Bme1580I G_kGCm'C
Cuts at: 0 508 583 628 891
Size: 508 75 45 263
Fragments arranged by size:
508 263 75 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 891
Size: 274 617
BsaHI Gr'CG_yC
Cuts at: 0 555 755 891
Size: 555 200 136
Fragments arranged by size:
555 200 136
BseYI C'CCAG_C
Cuts at: 0 576 696 840 891
Size: 576 120 144 51
Fragments arranged by size:
576 144 120 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 891
Size: 379 512
BsiHKAI G_wGCw'C
Cuts at: 0 710 891
Size: 710 181
Bsp1286I G_dGCh'C
Cuts at: 0 508 583 628 710 851 891
Size: 508 75 45 82 141 40
Fragments arranged by size:
508 141 82 75 45 40
BsrBI CCG'CTC
Cuts at: 0 447 891
Size: 447 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 372 891
Size: 372 519
BstYI r'GATC_y
Cuts at: 0 680 891
Size: 680 211
BtsI GCAGTG_nn'
Cuts at: 0 708 891
Size: 708 183
EaeI y'GGCC_r
Cuts at: 0 380 891
Size: 380 511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 495 876 891
Size: 495 381 15
Fragments arranged by size:
495 381 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 891
Size: 274 617
EcoO109I rG'GnC_Cy
Cuts at: 0 25 891
Size: 25 866
EcoRI G'AATT_C
Cuts at: 0 184 891
Size: 184 707
HaeII r_GCGC'y
Cuts at: 0 558 758 891
Size: 558 200 133
Fragments arranged by size:
558 200 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 114 127 146 891
Size: 95 19 13 19 745
Fragments arranged by size:
745 95 19 19 13
HincII GTy'rAC
Cuts at: 0 233 891
Size: 233 658
KasI G'GCGC_C
Cuts at: 0 554 754 891
Size: 554 200 137
Fragments arranged by size:
554 200 137
MscI TGG'CCA
Cuts at: 0 382 891
Size: 382 509
MslI CAynn'nnrTG
Cuts at: 0 775 891
Size: 775 116
MspA1I CmG'CkG
Cuts at: 0 87 252 765 891
Size: 87 165 513 126
Fragments arranged by size:
513 165 126 87
NarI GG'CG_CC
Cuts at: 0 555 755 891
Size: 555 200 136
Fragments arranged by size:
555 200 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 594 891
Size: 594 297
PstI C_TGCA'G
Cuts at: 0 203 500 620 737 891
Size: 203 297 120 117 154
Fragments arranged by size:
297 203 154 120 117
PvuII CAG'CTG
Cuts at: 0 252 891
Size: 252 639
ScaI AGT'ACT
Cuts at: 0 814 891
Size: 814 77
SfcI C'TryA_G
Cuts at: 0 199 496 616 733 891
Size: 199 297 120 117 158
Fragments arranged by size:
297 199 158 120 117
SfoI GGC'GCC
Cuts at: 0 556 756 891
Size: 556 200 135
Fragments arranged by size:
556 200 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 832 891
Size: 832 59
TatI w'GTAC_w
Cuts at: 0 812 891
Size: 812 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 395 427 891
Size: 395 32 464
Fragments arranged by size:
464 395 32
Enzymes that do cut:
AccI AhdI AlwNI ApoI AvaI BanI BanII BbeI
BbsI BglI BglII Bme1580I BpmI BsaHI BseYI BsgI
BsiHKAI Bsp1286I BsrBI BstXI BstYI BtsI EaeI EciI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI PflMI PstI PvuII ScaI SfcI
SfoI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI