Restriction map for
pFN21AE4296
(Linear) (Six-base) MAPSORT of: KIEE4296.seq Check: 2515 from: 1 to: 9420
KIEE4296.orf
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3434 8239 8327 8773 9237 9420
Size: 3434 4805 88 446 464 183
Fragments arranged by size:
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AatII G_ACGT'C
Cuts at: 0 2981 9420
Size: 2981 6439
AccI GT'mk_AC
Cuts at: 0 4186 4940 6017 9420
Size: 4186 754 1077 3403
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 1747 9420
Size: 1747 7673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1128 1421 1703 1827 2169 2458 2607
Size: 790 338 293 282 124 342 289 149
Cuts at: 2607 3347 3451 3745 5078 5293 5899 5923 6106
Size: 740 104 294 1333 215 606 24 183
Cuts at: 6106 6398 8174 8792 9420
Size: 292 1776 618 628
Fragments arranged by size:
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338 294 293 292 289 282 215 183
149 124 104 24
AflII C'TTAA_G
Cuts at: 0 6438 9420
Size: 6438 2982
AflIII A'CryG_T
Cuts at: 0 2766 4312 6254 7219 7221 9216 9420
Size: 2766 1546 1942 965 2 1995 204
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 411 8838 9420
Size: 411 8427 582
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 7222 7236 9037 9420
Size: 7222 14 1801 383
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2437 6560 8335 9420
Size: 2437 4123 1775 1085
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 174 805 3646 5386 5511 6334 6645 6678
Size: 174 631 2841 1740 125 823 311 33
Cuts at: 6678 8036 8217 9060 9420
Size: 1358 181 843 360
Fragments arranged by size:
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181 174 125 33
ApaI G_GGCC'C
Cuts at: 0 3393 6666 9135 9420
Size: 3393 3273 2469 285
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 5985 6925 8182 8719 9420
Size: 5985 940 1257 537 701
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 342 352 1930 4541 5018 5193 6060 6458
Size: 342 10 1578 2611 477 175 867 398
Cuts at: 6458 6745 8542 9420
Size: 287 1797 878
Fragments arranged by size:
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287 175 10
AseI AT'TA_AT
Cuts at: 0 5126 9420
Size: 5126 4294
AvaI C'yCGr_G
Cuts at: 0 496 1159 4155 6568 6659 7801 9420
Size: 496 663 2996 2413 91 1142 1619
Fragments arranged by size:
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AvrII C'CTAG_G
Cuts at: 0 3873 6444 9420
Size: 3873 2571 2976
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 5930 7725 8039 8927 9420
Size: 5930 1795 314 888 493
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 526 957 1747 2368 3396 6526 7759 8220
Size: 526 431 790 621 1028 3130 1233 461
Cuts at: 8220 9420
Size: 1200
Fragments arranged by size:
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431
BanII G_rGCy'C
Cuts at: 0 503 1122 2676 3393 5090 5460 5688 6137
Size: 503 619 1554 717 1697 370 228 449
Cuts at: 6137 6666 7456 8340 9135 9314 9420
Size: 529 790 884 795 179 106
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 2441 2756 3076 3949 4204 4375 4799 5258
Size: 2441 315 320 873 255 171 424 459
Cuts at: 5258 6552 7472 8201 9420
Size: 1294 920 729 1219
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 140 149 182 236 2140 2332 9420
Size: 140 9 33 54 1904 192 7088
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6007 6041 6117 6151 6559 6593 7915 7949
Size: 6007 34 76 34 408 34 1322 34
Cuts at: 7949 9420
Size: 1471
Fragments arranged by size:
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34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3871 9420
Size: 3871 5549
BclI T'GATC_A
Cuts at: 0 1965 2540 5468 5774 8081 9420
Size: 1965 575 2928 306 2307 1339
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 2892 9420
Size: 2892 6528
BglI GCCn_nnn'nGGC
Cuts at: 0 998 1837 4612 7504 8743 9420
Size: 998 839 2775 2892 1239 677
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 5769 9420
Size: 5769 3651
BlpI GC'TnA_GC
Cuts at: 0 566 3798 7633 8055 9420
Size: 566 3232 3835 422 1365
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 531 962 2371 3393 3401 3453 5989 6254
Size: 531 431 1409 1022 8 52 2536 265
Cuts at: 6254 6483 6666 6929 7229 7764 8186 8628 8723
Size: 229 183 263 300 535 422 442 95
Cuts at: 8723 9135 9420
Size: 412 285
Fragments arranged by size:
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52 8
BmgBI CAC'GTC
Cuts at: 0 3456 3468 7955 9255 9420
Size: 3456 12 4487 1300 165
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 3097 3682 3966 4185 5043 6075 6306 6695
Size: 3097 585 284 219 858 1032 231 389
Cuts at: 6695 8931 9015 9105 9420
Size: 2236 84 90 315
Fragments arranged by size:
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231 219 90 84
BmtI G_CTAG'C
Cuts at: 0 6430 9420
Size: 6430 2990
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1371 5704 5974 8682 9420
Size: 1371 4333 270 2708 738
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 1144 1289 1436 1578 4020 4159 5636
Size: 332 812 145 147 142 2442 139 1477
Cuts at: 5636 5780 6203 6618 6802 6888 6958 6980 7297
Size: 144 423 415 184 86 70 22 317
Cuts at: 7297 7943 7953 8006 8314 8383 8659 9262 9420
Size: 646 10 53 308 69 276 603 158
Fragments arranged by size:
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142 139 86 70 69 53 22 10
Bpu10I CC'TnA_GC
Cuts at: 0 140 149 182 236 1214 2140 2332 6418
Size: 140 9 33 54 978 926 192 4086
Cuts at: 6418 7168 8305 9420
Size: 750 1137 1115
Fragments arranged by size:
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54 33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 819 1602 6784 8041 9361 9420
Size: 25 794 783 5182 1257 1320 59
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 642 664 2267 6693 6882 8118 9420
Size: 642 22 1603 4426 189 1236 1302
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 2352 6255 7222 8478 9152 9420
Size: 2352 3903 967 1256 674 268
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 476 4394 5347 9420
Size: 476 3918 953 4073
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 2978 4109 4984 7928 9420
Size: 2978 1131 875 2944 1492
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 1114 2100 4935 5369 6276 9420
Size: 1114 986 2835 434 907 3144
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 528 558 2144 2174 2889 2919 3108 3138
Size: 528 30 1586 30 715 30 189 30
Cuts at: 3138 3952 3982 6892 6922 7984 8014 9420
Size: 814 30 2910 30 1062 30 1406
Fragments arranged by size:
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BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 128 253 1234 1585 2303 3376 4023 5638
Size: 128 125 981 351 718 1073 647 1615
Cuts at: 5638 6206 6870 7030 7033 7036 7462 7906 7927
Size: 568 664 160 3 3 426 444 21
Cuts at: 7927 7930 7933 8413 8608 9319 9322 9420
Size: 3 3 480 195 711 3 98
Fragments arranged by size:
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98 21 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 3342 3997 7557 7812 8323 9420
Size: 3342 655 3560 255 511 1097
Fragments arranged by size:
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BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 417 2012 3635 4150 4250 4339 5575
Size: 232 185 1595 1623 515 100 89 1236
Cuts at: 5575 6320 6371 6553 6614 6973 7522 7919 8326
Size: 745 51 182 61 359 549 397 407
Cuts at: 8326 9046 9420
Size: 720 374
Fragments arranged by size:
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61 51
BsiEI CG_ry'CG
Cuts at: 0 9140 9318 9420
Size: 9140 178 102
Fragments arranged by size:
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BsiHKAI G_wGCw'C
Cuts at: 0 503 878 1325 2717 3054 3297 4020 4996
Size: 503 375 447 1392 337 243 723 976
Cuts at: 4996 5090 5738 5960 5989 6929 7526 8186 8302
Size: 94 648 222 29 940 597 660 116
Cuts at: 8302 8723 9314 9420
Size: 421 591 106
Fragments arranged by size:
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503 447 421 375 337 243 222 116
106 94 29
BsmI GAATG_Cn'
Cuts at: 0 445 2414 4245 6393 7285 9420
Size: 445 1969 1831 2148 892 2135
Fragments arranged by size:
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BsmBI CGTCTCn'nnnn_
Cuts at: 0 2890 6187 7961 9420
Size: 2890 3297 1774 1459
Fragments arranged by size:
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Bsp1286I G_dGCh'C
Cuts at: 0 503 531 878 962 1122 1325 2371 2676
Size: 503 28 347 84 160 203 1046 305
Cuts at: 2676 2717 3054 3297 3393 3401 3453 4020 4996
Size: 41 337 243 96 8 52 567 976
Cuts at: 4996 5090 5460 5688 5738 5960 5989 6137 6254
Size: 94 370 228 50 222 29 148 117
Cuts at: 6254 6483 6666 6929 7229 7456 7526 7764 8186
Size: 229 183 263 300 227 70 238 422
Cuts at: 8186 8302 8340 8628 8723 9135 9314 9420
Size: 116 38 288 95 412 179 106
Fragments arranged by size:
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337 305 300 288 263 243 238 229
228 227 222 203 183 179 160 148
117 116 106 96 95 94 84 70
52 50 41 38 29 28 8
BspEI T'CCGG_A
Cuts at: 0 2100 5369 6276 9420
Size: 2100 3269 907 3144
Fragments arranged by size:
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BspHI T'CATG_A
Cuts at: 0 1467 2495 3024 4875 6395 8972 9420
Size: 1467 1028 529 1851 1520 2577 448
Fragments arranged by size:
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BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 966 1453 2327 3434 4323 8239 8327 8381
Size: 966 487 874 1107 889 3916 88 54
Cuts at: 8381 8773 9237 9420
Size: 392 464 183
Fragments arranged by size:
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183 88 54
BsrBI CCG'CTC
Cuts at: 0 6524 7806 8862 9420
Size: 6524 1282 1056 558
Fragments arranged by size:
6524 1282 1056 558
BsrDI GCAATG_nn'
Cuts at: 0 7162 7538 7679 9420
Size: 7162 376 141 1741
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 4611 6585 7146 7549 9374 9420
Size: 4611 1974 561 403 1825 46
Fragments arranged by size:
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BsrGI T'GTAC_A
Cuts at: 0 2473 5116 8027 8771 9420
Size: 2473 2643 2911 744 649
Fragments arranged by size:
2911 2643 2473 744 649
BssSI C'ACGA_G
Cuts at: 0 4209 5788 7432 7774 9420
Size: 4209 1579 1644 342 1646
Fragments arranged by size:
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BstAPI GCAn_nnn'nTGC
Cuts at: 0 1506 3292 8016 9420
Size: 1506 1786 4724 1404
Fragments arranged by size:
4724 1786 1506 1404
BstEII G'GTnAC_C
Cuts at: 0 7253 9408 9420
Size: 7253 2155 12
Fragments arranged by size:
7253 2155 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 2740 3979 5614 7238 8919 9420
Size: 699 2041 1239 1635 1624 1681 501
Fragments arranged by size:
2041 1681 1635 1624 1239 699 501
BstYI r'GATC_y
Cuts at: 0 1524 3237 3756 3869 5769 5930 6797 6813
Size: 1524 1713 519 113 1900 161 867 16
Cuts at: 6813 7032 7295 7725 8039 8098 8201 8927 9420
Size: 219 263 430 314 59 103 726 493
Fragments arranged by size:
1900 1713 1524 867 726 519 493 430
314 263 219 161 113 103 59 16
Bsu36I CC'TnA_GG
Cuts at: 0 946 2228 3639 4117 7885 9420
Size: 946 1282 1411 478 3768 1535
Fragments arranged by size:
3768 1535 1411 1282 946 478
BtgI C'CryG_G
Cuts at: 0 1489 1628 1856 2216 2849 3401 7301 8267
Size: 1489 139 228 360 633 552 3900 966
Cuts at: 8267 8738 9035 9083 9143 9193 9420
Size: 471 297 48 60 50 227
Fragments arranged by size:
3900 1489 966 633 552 471 360 297
228 227 139 60 50 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 9420
Size: 357 9063
BtsI GCAGTG_nn'
Cuts at: 0 405 821 1504 1896 1926 2294 2874 3092
Size: 405 416 683 392 30 368 580 218
Cuts at: 3092 3609 3838 4780 5326 5412 6337 6956 7061
Size: 517 229 942 546 86 925 619 105
Cuts at: 7061 8384 8448 8495 9420
Size: 1323 64 47 925
Fragments arranged by size:
1323 942 925 925 683 619 580 546
517 416 405 392 368 229 218 105
86 64 47 30
ClaI AT'CG_AT
Cuts at: 0 2264 2546 9420
Size: 2264 282 6874
Fragments arranged by size:
6874 2264 282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6873 6908 7389 7424 9420
Size: 6873 35 481 35 1996
Fragments arranged by size:
6873 1996 481 35 35
DraI TTT'AAA
Cuts at: 0 2696 4292 9420
Size: 2696 1596 5128
Fragments arranged by size:
5128 2696 1596
DraIII CAC_nnn'GTG
Cuts at: 0 3151 6924 7857 8454 9420
Size: 3151 3773 933 597 966
Fragments arranged by size:
3773 3151 966 933 597
EaeI y'GGCC_r
Cuts at: 0 1230 1529 2846 3191 3269 3370 3833 4577
Size: 1230 299 1317 345 78 101 463 744
Cuts at: 4577 5024 6468 7496 7547 7836 8072 8921 9029
Size: 447 1444 1028 51 289 236 849 108
Cuts at: 9029 9146 9420
Size: 117 274
Fragments arranged by size:
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345 299 289 274 236 117 108 101
78 51
EarI CTCTTCn'nnn_
Cuts at: 0 1147 1857 3166 3421 3860 5615 5673 5701
Size: 1147 710 1309 255 439 1755 58 28
Cuts at: 5701 5918 6231 6469 7904 9420
Size: 217 313 238 1435 1516
Fragments arranged by size:
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255 238 217 58 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 810 1528 2038 2422 4150 8287 9420
Size: 810 718 510 384 1728 4137 1133
Fragments arranged by size:
4137 1728 1133 810 718 510 384
EcoICRI GAG'CTC
Cuts at: 0 501 5088 9312 9420
Size: 501 4587 4224 108
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 790 1128 1144 1289 1421 1436 1578
Size: 332 458 338 16 145 132 15 142
Cuts at: 1578 1703 1827 2169 2458 2607 3347 3451 3745
Size: 125 124 342 289 149 740 104 294
Cuts at: 3745 4020 4159 5078 5293 5636 5780 5899 5923
Size: 275 139 919 215 343 144 119 24
Cuts at: 5923 6106 6203 6398 6618 6802 6888 6958 6980
Size: 183 97 195 220 184 86 70 22
Cuts at: 6980 7297 7943 7953 8006 8174 8314 8383 8659
Size: 317 646 10 53 168 140 69 276
Cuts at: 8659 8792 9262 9420
Size: 133 470 158
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332 317 294 289 276 275 220 215
195 184 183 168 158 149 145 144
142 140 139 133 132 125 124 119
104 97 86 70 69 53 24 22
16 15 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1867 3430 7888 9420
Size: 1867 1563 4458 1532
Fragments arranged by size:
4458 1867 1563 1532
EcoO109I rG'GnC_Cy
Cuts at: 0 1646 1696 2130 2312 2339 3390 3643 4098
Size: 1646 50 434 182 27 1051 253 455
Cuts at: 4098 6663 6768 7478 8066 9131 9132 9323 9420
Size: 2565 105 710 588 1065 1 191 97
Fragments arranged by size:
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253 191 182 105 97 50 27 1
EcoRI G'AATT_C
Cuts at: 0 4541 5018 9420
Size: 4541 477 4402
Fragments arranged by size:
4541 4402 477
EcoRV GAT'ATC
Cuts at: 0 1578 5012 9420
Size: 1578 3434 4408
Fragments arranged by size:
4408 3434 1578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2224 3513 3966 6220 7344 9420
Size: 2224 1289 453 2254 1124 2076
Fragments arranged by size:
2254 2224 2076 1289 1124 453
HaeII r_GCGC'y
Cuts at: 0 1610 4026 8646 9420
Size: 1610 2416 4620 774
Fragments arranged by size:
4620 2416 1610 774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 895 927 1065 1097 1345 1358
Size: 397 32 466 32 138 32 248 13
Cuts at: 1358 1377 1390 1816 1848 2008 2040 2889 2921
Size: 19 13 426 32 160 32 849 32
Cuts at: 2921 3669 3701 3970 4002 6757 6789 6798 6830
Size: 748 32 269 32 2755 32 9 32
Cuts at: 6830 7979 7984 8011 8016 8193 8225 8668 8700
Size: 1149 5 27 5 177 32 443 32
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Size: 124 27 5 27 100 32 405
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32 32 32 32 32 32 32 32
32 32 32 32 32 27 27 27
19 13 13 9 5 5 5
HincII GTy'rAC
Cuts at: 0 5054 5523 6018 7209 7965 7992 9420
Size: 5054 469 495 1191 756 27 1428
Fragments arranged by size:
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HindIII A'AGCT_T
Cuts at: 0 461 3074 4286 7243 9420
Size: 461 2613 1212 2957 2177
Fragments arranged by size:
2957 2613 2177 1212 461
KpnI G_GTAC'C
Cuts at: 0 1751 9420
Size: 1751 7669
MfeI C'AATT_G
Cuts at: 0 3934 9420
Size: 3934 5486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3561 4016 4727 5917 6298 8313 8453 9026
Size: 3561 455 711 1190 381 2015 140 573
Cuts at: 9026 9140 9420
Size: 114 280
Fragments arranged by size:
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140 114
MscI TGG'CCA
Cuts at: 0 1531 3193 3372 3835 4579 5026 6470 8923
Size: 1531 1662 179 463 744 447 1444 2453
Cuts at: 8923 9031 9148 9420
Size: 108 117 272
Fragments arranged by size:
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179 117 108
MslI CAynn'nnrTG
Cuts at: 0 376 2548 3063 3254 4441 7222 7236 8767
Size: 376 2172 515 191 1187 2781 14 1531
Cuts at: 8767 9037 9420
Size: 270 383
Fragments arranged by size:
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191 14
MspA1I CmG'CkG
Cuts at: 0 174 246 685 802 1210 1535 1708 2144
Size: 174 72 439 117 408 325 173 436
Cuts at: 2144 2158 2479 2538 4356 5271 5386 5540 5843
Size: 14 321 59 1818 915 115 154 303
Cuts at: 5843 6131 6237 6804 6855 7561 8036 8242 9420
Size: 288 106 567 51 706 475 206 1178
Fragments arranged by size:
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408 325 321 303 288 206 174 173
154 117 115 106 72 59 51 14
NaeI GCC'GGC
Cuts at: 0 7148 7551 9420
Size: 7148 403 1869
Fragments arranged by size:
7148 1869 403
NcoI C'CATG_G
Cuts at: 0 1628 1856 3401 8738 9083 9143 9420
Size: 1628 228 1545 5337 345 60 277
Fragments arranged by size:
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NgoMIV G'CCGG_C
Cuts at: 0 7146 7549 9420
Size: 7146 403 1871
Fragments arranged by size:
7146 1871 403
NheI G'CTAG_C
Cuts at: 0 6426 9420
Size: 6426 2994
NsiI A_TGCA'T
Cuts at: 0 2894 9420
Size: 2894 6526
NspI r_CATG'y
Cuts at: 0 2770 4316 4606 4947 8772 9220 9420
Size: 2770 1546 290 341 3825 448 200
Fragments arranged by size:
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PasI CC'CwG_GG
Cuts at: 0 2756 3378 9420
Size: 2756 622 6042
Fragments arranged by size:
6042 2756 622
PciI A'CATG_T
Cuts at: 0 2766 4312 9216 9420
Size: 2766 1546 4904 204
Fragments arranged by size:
4904 2766 1546 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 1764 3459 6237 9027 9194 9420
Size: 1764 1695 2778 2790 167 226
Fragments arranged by size:
2790 2778 1764 1695 226 167
PfoI T'CCnGG_A
Cuts at: 0 665 3458 3474 6110 6935 7313 7969 9089
Size: 665 2793 16 2636 825 378 656 1120
Cuts at: 9089 9420
Size: 331
Fragments arranged by size:
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16
PmlI CAC'GTG
Cuts at: 0 2352 6255 7222 9152 9420
Size: 2352 3903 967 1930 268
Fragments arranged by size:
3903 2352 1930 967 268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2553 2585 2699 2731 4138 4170 9420
Size: 2553 32 114 32 1407 32 5250
Fragments arranged by size:
5250 2553 1407 114 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1646 2312 2339 3643 7478 8066 9420
Size: 1646 666 27 1304 3835 588 1354
Fragments arranged by size:
3835 1646 1354 1304 666 588 27
PshAI GACnn'nnGTC
Cuts at: 0 1183 9420
Size: 1183 8237
PsiI TTA'TAA
Cuts at: 0 2813 4282 9420
Size: 2813 1469 5138
Fragments arranged by size:
5138 2813 1469
PspOMI G'GGCC_C
Cuts at: 0 3389 6662 9131 9420
Size: 3389 3273 2469 289
Fragments arranged by size:
3389 3273 2469 289
PspXI vC'TCGA_Gb
Cuts at: 0 496 9420
Size: 496 8924
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1061 1093 9420
Size: 1061 32 8327
Fragments arranged by size:
8327 1061 32
PstI C_TGCA'G
Cuts at: 0 684 971 1047 1511 1775 1903 2509 2537
Size: 684 287 76 464 264 128 606 28
Cuts at: 2537 3845 4622 4772 6582 6854 7068 7106 7793
Size: 1308 777 150 1810 272 214 38 687
Cuts at: 7793 8826 9420
Size: 1033 594
Fragments arranged by size:
1810 1308 1033 777 687 684 606 594
464 287 272 264 214 150 128 76
38 28
PvuII CAG'CTG
Cuts at: 0 685 1535 1708 2144 2158 2479 2538 4356
Size: 685 850 173 436 14 321 59 1818
Cuts at: 4356 5271 5386 6855 7561 8242 9420
Size: 915 115 1469 706 681 1178
Fragments arranged by size:
1818 1469 1178 915 850 706 685 681
436 321 173 115 59 14
SacI G_AGCT'C
Cuts at: 0 503 5090 9314 9420
Size: 503 4587 4224 106
Fragments arranged by size:
4587 4224 503 106
SalI G'TCGA_C
Cuts at: 0 6016 9420
Size: 6016 3404
SanDI GG'GwC_CC
Cuts at: 0 1646 2312 9420
Size: 1646 666 7108
Fragments arranged by size:
7108 1646 666
SapI GCTCTTCn'nnn_
Cuts at: 0 1147 3166 3421 9420
Size: 1147 2019 255 5999
Fragments arranged by size:
5999 2019 1147 255
ScaI AGT'ACT
Cuts at: 0 5151 9420
Size: 5151 4269
SexAI A'CCwGG_T
Cuts at: 0 578 608 6322 6677 7615 8216 9420
Size: 578 30 5714 355 938 601 1204
Fragments arranged by size:
5714 1204 938 601 578 355 30
SfcI C'TryA_G
Cuts at: 0 680 855 967 1043 1507 1771 1899 2505
Size: 680 175 112 76 464 264 128 606
Cuts at: 2505 2517 2533 3841 4618 4768 6578 6850 7064
Size: 12 16 1308 777 150 1810 272 214
Cuts at: 7064 7102 7754 7789 8822 9288 9420
Size: 38 652 35 1033 466 132
Fragments arranged by size:
1810 1308 1033 777 680 652 606 466
464 272 264 214 175 150 132 128
112 76 38 35 16 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 7504 9420
Size: 7504 1916
SmaI CCC'GGG
Cuts at: 0 6661 9420
Size: 6661 2759
SmlI C'TyrA_G
Cuts at: 0 40 496 834 1581 6438 6763 8020 9340
Size: 40 456 338 747 4857 325 1257 1320
Cuts at: 9340 9420
Size: 80
Fragments arranged by size:
4857 1320 1257 747 456 338 325 80
40
SrfI GCCC'GGGC
Cuts at: 0 6661 9420
Size: 6661 2759
SspI AAT'ATT
Cuts at: 0 850 7695 9420
Size: 850 6845 1725
Fragments arranged by size:
6845 1725 850
StuI AGG'CCT
Cuts at: 0 28 603 754 1654 1840 8636 9420
Size: 28 575 151 900 186 6796 784
Fragments arranged by size:
6796 900 784 575 186 151 28
StyI C'CwwG_G
Cuts at: 0 484 674 1343 1628 1856 2823 3401 3803
Size: 484 190 669 285 228 967 578 402
Cuts at: 3803 3873 4026 4884 6444 7118 7407 8250 8738
Size: 70 153 858 1560 674 289 843 488
Cuts at: 8738 9083 9143 9420
Size: 345 60 277
Fragments arranged by size:
1560 967 858 843 674 669 578 488
484 402 345 289 285 277 228 190
153 70 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4099 4108 5491 7272 7717 7965 9420
Size: 4099 9 1383 1781 445 248 1455
Fragments arranged by size:
4099 1781 1455 1383 445 248 9
TatI w'GTAC_w
Cuts at: 0 2473 4298 5116 5149 5799 8027 8771 9420
Size: 2473 1825 818 33 650 2228 744 649
Fragments arranged by size:
2473 2228 1825 818 744 650 649 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 528 560 800 832 2021 2053 2075 2107
Size: 528 32 240 32 1189 32 22 32
Cuts at: 2107 2144 2176 5566 5598 6892 6924 7032 7064
Size: 37 32 3390 32 1294 32 108 32
Cuts at: 7064 9420
Size: 2356
Fragments arranged by size:
3390 2356 1294 1189 528 240 108 37
32 32 32 32 32 32 32 32
22
Tth111I GACn'n_nGTC
Cuts at: 0 5576 6324 9097 9420
Size: 5576 748 2773 323
Fragments arranged by size:
5576 2773 748 323
XbaI T'CTAG_A
Cuts at: 0 3622 8943 9420
Size: 3622 5321 477
Fragments arranged by size:
5321 3622 477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3110 7515 8745 9420
Size: 3110 4405 1230 675
Fragments arranged by size:
4405 3110 1230 675
XhoI C'TCGA_G
Cuts at: 0 496 9420
Size: 496 8924
XmaI C'CCGG_G
Cuts at: 0 6659 9420
Size: 6659 2761
XmnI GAAnn'nnTTC
Cuts at: 0 28 386 6047 6119 6391 9262 9420
Size: 28 358 5661 72 272 2871 158
Fragments arranged by size:
5661 2871 358 272 158 72 28
ZraI GAC'GTC
Cuts at: 0 2979 9420
Size: 2979 6441
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KpnI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuII SacI
SalI SanDI SapI ScaI SexAI SfcI SfiI SmaI
SmlI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AloI AscI AsiSI BaeI BbeI
BsiWI BssHII BstBI BstZ17I DrdI EagI FseI FspI
FspAI HpaI KasI MluI NarI NdeI NotI NruI
PacI PmeI PvuI RsrII SacII SbfI SfoI SgfI
SgrAI SnaBI SpeI SphI SwaI