Restriction map for
pFN21AE4286
(Linear) (Six-base) MAPSORT of: KIEE4286.seq Check: 8458 from: 1 to: 4893
KIEE4286.orf
With 182 enzymes: SgfI *
July 3, 2012 18:56 ..
AccI GT'mk_AC
Cuts at: 0 416 740 4893
Size: 416 324 4153
Fragments arranged by size:
4153 416 324
Acc65I G'GTAC_C
Cuts at: 0 275 998 1691 4893
Size: 275 723 693 3202
Fragments arranged by size:
3202 723 693 275
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 549 947 2644 3472 4659 4893
Size: 163 386 398 1697 828 1187 234
Fragments arranged by size:
1697 1187 828 398 386 234 163
AfeI AGC'GCT
Cuts at: 0 124 4893
Size: 124 4769
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 101 2264 4893
Size: 101 2163 2629
Fragments arranged by size:
2629 2163 101
AlwNI CAG_nnn'CTG
Cuts at: 0 99 1044 2670 3223 4069 4479 4626 4893
Size: 99 945 1626 553 846 410 147 267
Fragments arranged by size:
1626 945 846 553 410 267 147 99
ApaI G_GGCC'C
Cuts at: 0 22 1359 2403 4893
Size: 22 1337 1044 2490
Fragments arranged by size:
2490 1337 1044 22
ApoI r'AATT_y
Cuts at: 0 496 3416 3472 4893
Size: 496 2920 56 1421
Fragments arranged by size:
2920 1421 496 56
AvaI C'yCGr_G
Cuts at: 0 407 1367 2117 3325 4893
Size: 407 960 750 1208 1568
Fragments arranged by size:
1568 1208 960 750 407
BamHI G'GATC_C
Cuts at: 0 1743 4893
Size: 1743 3150
BanI G'GyrC_C
Cuts at: 0 275 998 1691 3765 4893
Size: 275 723 693 2074 1128
Fragments arranged by size:
2074 1128 723 693 275
BanII G_rGCy'C
Cuts at: 0 22 599 888 925 1310 1359 1959 2403
Size: 22 577 289 37 385 49 600 444
Cuts at: 2403 3600 3932 4893
Size: 1197 332 961
Fragments arranged by size:
1197 961 600 577 444 385 332 289
49 37 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 1630 3200 4893
Size: 1630 1570 1693
Fragments arranged by size:
1693 1630 1570
BbvCI CC'TCA_GC
Cuts at: 0 53 506 1421 2609 4893
Size: 53 453 915 1188 2284
Fragments arranged by size:
2284 1188 915 453 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3348 3382 4893
Size: 3348 34 1511
Fragments arranged by size:
3348 1511 34
BclI T'GATC_A
Cuts at: 0 1113 3906 4893
Size: 1113 2793 987
Fragments arranged by size:
2793 1113 987
BglI GCCn_nnn'nGGC
Cuts at: 0 883 1363 2225 3628 4893
Size: 883 480 862 1403 1265
Fragments arranged by size:
1403 1265 883 862 480
BglII A'GATC_T
Cuts at: 0 251 4656 4893
Size: 251 4405 237
Fragments arranged by size:
4405 251 237
BlpI GC'TnA_GC
Cuts at: 0 2252 2833 3205 3638 4619 4893
Size: 2252 581 372 433 981 274
Fragments arranged by size:
2252 981 581 433 372 274
Bme1580I G_kGCm'C
Cuts at: 0 22 1207 1359 2403 3153 4001 4893
Size: 22 1185 152 1044 750 848 892
Fragments arranged by size:
1185 1044 892 848 750 152 22
BmgBI CAC'GTC
Cuts at: 0 3081 4893
Size: 3081 1812
BmrI ACTGGGnnnn_n'
Cuts at: 0 692 4893
Size: 692 4201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1347 1844 4893
Size: 1347 497 3049
Fragments arranged by size:
3049 1347 497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 193 535 730 1024 1208 1258 1279
Size: 130 63 342 195 294 184 50 21
Cuts at: 1279 1381 1407 1769 2194 2362 2614 2692 2872
Size: 102 26 362 425 168 252 78 180
Cuts at: 2872 2976 3004 3025 3736 3901 3955 4780 4893
Size: 104 28 21 711 165 54 825 113
Fragments arranged by size:
825 711 425 362 342 294 252 195
184 180 168 165 130 113 104 102
78 63 54 50 28 26 21 21
Bpu10I CC'TnA_GC
Cuts at: 0 53 506 1421 1504 2609 4009 4480 4893
Size: 53 453 915 83 1105 1400 471 413
Fragments arranged by size:
1400 1105 915 471 453 413 83 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1786 3336 3715 4138 4893
Size: 922 864 1550 379 423 755
Fragments arranged by size:
1550 922 864 755 423 379
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 2751 4893
Size: 367 2384 2142
Fragments arranged by size:
2384 2142 367
BsaHI Gr'CG_yC
Cuts at: 0 526 2436 3482 3563 4221 4893
Size: 526 1910 1046 81 658 672
Fragments arranged by size:
1910 1046 672 658 526 81
BsaWI w'CCGG_w
Cuts at: 0 1163 2723 4245 4893
Size: 1163 1560 1522 648
Fragments arranged by size:
1560 1522 1163 648
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 2116 2146 2974 3004 3347 3377
Size: 1186 30 900 30 828 30 343 30
Cuts at: 3377 4150 4180 4893
Size: 773 30 713
Fragments arranged by size:
1186 900 828 773 713 343 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 70 127 163 190 993 1318 1690
Size: 21 49 57 36 27 803 325 372
Cuts at: 1690 1783 2137 2537 3355 3793 4519 4858 4893
Size: 93 354 400 818 438 726 339 35
Fragments arranged by size:
818 803 726 438 400 372 354 339
325 93 57 49 36 35 27 21
BseYI C'CCAG_C
Cuts at: 0 26 210 453 1044 1214 1442 2283 3543
Size: 26 184 243 591 170 228 841 1260
Cuts at: 3543 3611 4893
Size: 68 1282
Fragments arranged by size:
1282 1260 841 591 243 228 184 170
68 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 2425 4893
Size: 1162 1263 2468
Fragments arranged by size:
2468 1263 1162
BsiHKAI G_wGCw'C
Cuts at: 0 599 705 1274 1310 3206 3887 3932 4105
Size: 599 106 569 36 1896 681 45 173
Cuts at: 4105 4486 4893
Size: 381 407
Fragments arranged by size:
1896 681 599 569 407 381 173 106
45 36
BsmI GAATG_Cn'
Cuts at: 0 2256 4893
Size: 2256 2637
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3330 4893
Size: 3330 1563
Bsp1286I G_dGCh'C
Cuts at: 0 22 599 705 888 925 1207 1274 1310
Size: 22 577 106 183 37 282 67 36
Cuts at: 1310 1359 1959 2403 3153 3206 3600 3887 3932
Size: 49 600 444 750 53 394 287 45
Cuts at: 3932 4001 4105 4486 4893
Size: 69 104 381 407
Fragments arranged by size:
750 600 577 444 407 394 381 287
282 183 106 104 69 67 53 49
45 37 36 22
BspEI T'CCGG_A
Cuts at: 0 1163 4893
Size: 1163 3730
BspHI T'CATG_A
Cuts at: 0 4817 4893
Size: 4817 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1526 2891 4337 4893
Size: 1526 1365 1446 556
Fragments arranged by size:
1526 1446 1365 556
BsrBI CCG'CTC
Cuts at: 0 1887 4268 4893
Size: 1887 2381 625
Fragments arranged by size:
2381 1887 625
BsrDI GCAATG_nn'
Cuts at: 0 3896 4893
Size: 3896 997
BsrFI r'CCGG_y
Cuts at: 0 104 4893
Size: 104 4789
BsrGI T'GTAC_A
Cuts at: 0 2978 3836 4231 4893
Size: 2978 858 395 662
Fragments arranged by size:
2978 858 662 395
BstAPI GCAn_nnn'nTGC
Cuts at: 0 622 784 4893
Size: 622 162 4109
Fragments arranged by size:
4109 622 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 286 1416 2907 2955 3831 4743 4893
Size: 286 1130 1491 48 876 912 150
Fragments arranged by size:
1491 1130 912 876 286 150 48
BstYI r'GATC_y
Cuts at: 0 251 1743 1772 2069 2126 2297 2387 2477
Size: 251 1492 29 297 57 171 90 90
Cuts at: 2477 2651 3939 4160 4656 4782 4893
Size: 174 1288 221 496 126 111
Fragments arranged by size:
1492 1288 496 297 251 221 174 171
126 111 90 90 57 29
Bsu36I CC'TnA_GG
Cuts at: 0 1208 2431 4893
Size: 1208 1223 2462
Fragments arranged by size:
2462 1223 1208
BtgI C'CryG_G
Cuts at: 0 233 377 879 1970 2223 3581 3830 3845
Size: 233 144 502 1091 253 1358 249 15
Cuts at: 3845 4893
Size: 1048
Fragments arranged by size:
1358 1091 1048 502 253 249 233 144
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3333 3969 4893
Size: 3333 636 924
Fragments arranged by size:
3333 924 636
BtsI GCAGTG_nn'
Cuts at: 0 656 1274 1913 2033 2150 2751 2969 3797
Size: 656 618 639 120 117 601 218 828
Cuts at: 3797 4252 4610 4893
Size: 455 358 283
Fragments arranged by size:
828 656 639 618 601 455 358 283
218 120 117
DraI TTT'AAA
Cuts at: 0 2361 2825 4893
Size: 2361 464 2068
Fragments arranged by size:
2361 2068 464
DraIII CAC_nnn'GTG
Cuts at: 0 1140 4893
Size: 1140 3753
DrdI GACnn_nn'nnGTC
Cuts at: 0 3496 4893
Size: 3496 1397
EaeI y'GGCC_r
Cuts at: 0 203 536 587 1296 2909 3440 3578 3821
Size: 203 333 51 709 1613 531 138 243
Cuts at: 3821 4118 4397 4893
Size: 297 279 496
Fragments arranged by size:
1613 709 531 496 333 297 279 243
203 138 51
EarI CTCTTCn'nnn_
Cuts at: 0 445 451 710 1315 1553 2630 2881 4607
Size: 445 6 259 605 238 1077 251 1726
Cuts at: 4607 4831 4893
Size: 224 62
Fragments arranged by size:
1726 1077 605 445 259 251 238 224
62 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1765 4452 4893
Size: 1765 2687 441
Fragments arranged by size:
2687 1765 441
EcoICRI GAG'CTC
Cuts at: 0 597 1308 3930 4893
Size: 597 711 2622 963
Fragments arranged by size:
2622 963 711 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 163 193 535 549 730 947 1024
Size: 130 33 30 342 14 181 217 77
Cuts at: 1024 1208 1258 1279 1381 1407 1769 2194 2362
Size: 184 50 21 102 26 362 425 168
Cuts at: 2362 2614 2644 2692 2872 2976 3004 3025 3472
Size: 252 30 48 180 104 28 21 447
Cuts at: 3472 3736 3901 3955 4659 4780 4893
Size: 264 165 54 704 121 113
Fragments arranged by size:
704 447 425 362 342 264 252 217
184 181 180 168 165 130 121 113
104 102 77 54 50 48 33 30
30 28 26 21 21 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 838 1409 1420 4630 4893
Size: 145 693 571 11 3210 263
Fragments arranged by size:
3210 693 571 263 145 11
EcoO109I rG'GnC_Cy
Cuts at: 0 942 1356 1572 2273 2679 3699 4476 4893
Size: 942 414 216 701 406 1020 777 417
Fragments arranged by size:
1020 942 777 701 417 414 406 216
EcoRV GAT'ATC
Cuts at: 0 4125 4893
Size: 4125 768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2937 4512 4656 4893
Size: 2937 1575 144 237
Fragments arranged by size:
2937 1575 237 144
HaeII r_GCGC'y
Cuts at: 0 126 3618 4364 4893
Size: 126 3492 746 529
Fragments arranged by size:
3492 746 529 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 494 1728 1760 2116 2148 2289 2321
Size: 462 32 1234 32 356 32 141 32
Cuts at: 2321 3332 3364 3787 3819 4150 4182 4893
Size: 1011 32 423 32 331 32 711
Fragments arranged by size:
1234 1011 711 462 423 356 331 141
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 873 2463 3861 4851 4893
Size: 873 1590 1398 990 42
Fragments arranged by size:
1590 1398 990 873 42
HpaI GTT'AAC
Cuts at: 0 2463 4893
Size: 2463 2430
KpnI G_GTAC'C
Cuts at: 0 279 1002 1695 4893
Size: 279 723 693 3198
Fragments arranged by size:
3198 723 693 279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4103 4893
Size: 4103 790
MscI TGG'CCA
Cuts at: 0 205 538 589 1298 2911 3442 3580 3823
Size: 205 333 51 709 1613 531 138 243
Cuts at: 3823 4399 4893
Size: 576 494
Fragments arranged by size:
1613 709 576 531 494 333 243 205
138 51
MslI CAynn'nnrTG
Cuts at: 0 3844 4893
Size: 3844 1049
MspA1I CmG'CkG
Cuts at: 0 16 52 270 505 555 628 1011 1146
Size: 16 36 218 235 50 73 383 135
Cuts at: 1146 1218 1257 1336 1654 2137 2328 2670 2787
Size: 72 39 79 318 483 191 342 117
Cuts at: 2787 3024 3547 3589 4113 4209 4311 4893
Size: 237 523 42 524 96 102 582
Fragments arranged by size:
582 524 523 483 383 342 318 237
235 218 191 135 117 102 96 79
73 72 50 42 39 36 16
NaeI GCC'GGC
Cuts at: 0 106 4893
Size: 106 4787
NcoI C'CATG_G
Cuts at: 0 879 1970 3581 3830 4893
Size: 879 1091 1611 249 1063
Fragments arranged by size:
1611 1091 1063 879 249
NdeI CA'TA_TG
Cuts at: 0 3291 4893
Size: 3291 1602
NgoMIV G'CCGG_C
Cuts at: 0 104 4893
Size: 104 4789
NspI r_CATG'y
Cuts at: 0 405 2536 4893
Size: 405 2131 2357
Fragments arranged by size:
2357 2131 405
PasI CC'CwG_GG
Cuts at: 0 3624 4347 4893
Size: 3624 723 546
Fragments arranged by size:
3624 723 546
PflMI CCAn_nnn'nTGG
Cuts at: 0 125 1785 3081 3624 4893
Size: 125 1660 1296 543 1269
Fragments arranged by size:
1660 1296 1269 543 125
PfoI T'CCnGG_A
Cuts at: 0 1746 2630 3041 3179 3932 4625 4893
Size: 1746 884 411 138 753 693 268
Fragments arranged by size:
1746 884 753 693 411 268 138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1910 1942 4893
Size: 1910 32 2951
Fragments arranged by size:
2951 1910 32
PpuMI rG'GwC_Cy
Cuts at: 0 1572 2273 2679 3699 4893
Size: 1572 701 406 1020 1194
Fragments arranged by size:
1572 1194 1020 701 406
PspOMI G'GGCC_C
Cuts at: 0 18 1355 2399 4893
Size: 18 1337 1044 2494
Fragments arranged by size:
2494 1337 1044 18
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3508 3540 4893
Size: 3508 32 1353
Fragments arranged by size:
3508 1353 32
PstI C_TGCA'G
Cuts at: 0 618 1098 1523 1653 2057 2738 3158 3993
Size: 618 480 425 130 404 681 420 835
Cuts at: 3993 4463 4793 4893
Size: 470 330 100
Fragments arranged by size:
835 681 618 480 470 425 420 404
330 130 100
PvuII CAG'CTG
Cuts at: 0 52 270 505 555 628 1011 1218 1257
Size: 52 218 235 50 73 383 207 39
Cuts at: 1257 1336 1654 2137 2670 2787 3024 3547 3589
Size: 79 318 483 533 117 237 523 42
Cuts at: 3589 4113 4209 4893
Size: 524 96 684
Fragments arranged by size:
684 533 524 523 483 383 318 237
235 218 207 117 96 79 73 52
50 42 39
SacI G_AGCT'C
Cuts at: 0 599 1310 3932 4893
Size: 599 711 2622 961
Fragments arranged by size:
2622 961 711 599
SapI GCTCTTCn'nnn_
Cuts at: 0 710 1315 4893
Size: 710 605 3578
Fragments arranged by size:
3578 710 605
SbfI CC_TGCA'GG
Cuts at: 0 1523 3158 4893
Size: 1523 1635 1735
Fragments arranged by size:
1735 1635 1523
ScaI AGT'ACT
Cuts at: 0 3802 4893
Size: 3802 1091
SfcI C'TryA_G
Cuts at: 0 614 1094 1519 1649 2053 2517 2734 3154
Size: 614 480 425 130 404 464 217 420
Cuts at: 3154 3646 3989 4459 4521 4789 4893
Size: 492 343 470 62 268 104
Fragments arranged by size:
614 492 480 470 464 425 420 404
343 268 217 130 104 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1363 4893
Size: 1363 3530
SmaI CCC'GGG
Cuts at: 0 409 1369 2119 4893
Size: 409 960 750 2774
Fragments arranged by size:
2774 960 750 409
SmlI C'TyrA_G
Cuts at: 0 937 1765 3315 3730 4153 4893
Size: 937 828 1550 415 423 740
Fragments arranged by size:
1550 937 828 740 423 415
SphI G_CATG'C
Cuts at: 0 2536 4893
Size: 2536 2357
StuI AGG'CCT
Cuts at: 0 136 1415 2395 3007 3313 3670 4228 4893
Size: 136 1279 980 612 306 357 558 665
Fragments arranged by size:
1279 980 665 612 558 357 306 136
StyI C'CwwG_G
Cuts at: 0 879 1970 2144 3581 3830 4400 4893
Size: 879 1091 174 1437 249 570 493
Fragments arranged by size:
1437 1091 879 570 493 249 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 1480 4735 4893
Size: 337 1143 3255 158
Fragments arranged by size:
3255 1143 337 158
TatI w'GTAC_w
Cuts at: 0 2978 3524 3800 3836 4231 4529 4893
Size: 2978 546 276 36 395 298 364
Fragments arranged by size:
2978 546 395 364 298 276 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 4893
Size: 1186 32 3675
Fragments arranged by size:
3675 1186 32
Tth111I GACn'n_nGTC
Cuts at: 0 1570 1629 3223 4893
Size: 1570 59 1594 1670
Fragments arranged by size:
1670 1594 1570 59
XbaI T'CTAG_A
Cuts at: 0 888 4893
Size: 888 4005
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 705 2667 2952 3421 4113 4893
Size: 126 579 1962 285 469 692 780
Fragments arranged by size:
1962 780 692 579 469 285 126
XmaI C'CCGG_G
Cuts at: 0 407 1367 2117 4893
Size: 407 960 750 2776
Fragments arranged by size:
2776 960 750 407
XmnI GAAnn'nnTTC
Cuts at: 0 4658 4825 4893
Size: 4658 167 68
Fragments arranged by size:
4658 167 68
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PsrI PstI PvuII SacI SapI
SbfI ScaI SfcI SfiI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BfrBI BmtI BsaAI BsaBI BsiEI BsiWI BssHII
BssSI BstBI BstEII BstZ17I ClaI CspCI EagI EcoRI
FseI FspI FspAI HindIII KasI MfeI MluI NarI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XhoI ZraI