Restriction map for
pFN21AE4273
(Linear) (Six-base) MAPSORT of: KIEE4273.seq Check: 3071 from: 1 to: 3567
KIEE4273.orf
With 182 enzymes: SgfI *
July 3, 2012 18:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2311 3567
Size: 2311 1256
AccI GT'mk_AC
Cuts at: 0 771 1238 1433 2955 3567
Size: 771 467 195 1522 612
Fragments arranged by size:
1522 771 612 467 195
Acc65I G'GTAC_C
Cuts at: 0 1673 3567
Size: 1673 1894
AclI AA'CG_TT
Cuts at: 0 1007 3567
Size: 1007 2560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1412 1552 1724 2543 2762 3567
Size: 839 573 140 172 819 219 805
Fragments arranged by size:
839 819 805 573 219 172 140
AflIII A'CryG_T
Cuts at: 0 1032 2373 3567
Size: 1032 1341 1194
Fragments arranged by size:
1341 1194 1032
AhdI GACnn_n'nnGTC
Cuts at: 0 3353 3567
Size: 3353 214
AleI CACnn'nnGTG
Cuts at: 0 3055 3567
Size: 3055 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2271 3567
Size: 2271 1296
AlwNI CAG_nnn'CTG
Cuts at: 0 3052 3567
Size: 3052 515
ApoI r'AATT_y
Cuts at: 0 441 633 814 1082 1290 1850 2182 3228
Size: 441 192 181 268 208 560 332 1046
Cuts at: 3228 3508 3567
Size: 280 59
Fragments arranged by size:
1046 560 441 332 280 268 208 192
181 59
AseI AT'TA_AT
Cuts at: 0 3178 3208 3567
Size: 3178 30 359
Fragments arranged by size:
3178 359 30
BanI G'GyrC_C
Cuts at: 0 1673 2480 3567
Size: 1673 807 1087
Fragments arranged by size:
1673 1087 807
BanII G_rGCy'C
Cuts at: 0 470 3567
Size: 470 3097
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 1526 2800 3567
Size: 248 1278 1274 767
Fragments arranged by size:
1278 1274 767 248
BbvCI CC'TCA_GC
Cuts at: 0 535 3567
Size: 535 3032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2787 2821 3567
Size: 2787 34 746
Fragments arranged by size:
2787 746 34
BclI T'GATC_A
Cuts at: 0 975 1593 1665 3567
Size: 975 618 72 1902
Fragments arranged by size:
1902 975 618 72
BglI GCCn_nnn'nGGC
Cuts at: 0 736 3567
Size: 736 2831
BlpI GC'TnA_GC
Cuts at: 0 2783 3567
Size: 2783 784
BmgBI CAC'GTC
Cuts at: 0 2058 3567
Size: 2058 1509
BmrI ACTGGGnnnn_n'
Cuts at: 0 1340 2045 3567
Size: 1340 705 1522
Fragments arranged by size:
1522 1340 705
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 560 2632 3567
Size: 560 2072 935
Fragments arranged by size:
2072 935 560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1844 2198 3567
Size: 1844 354 1369
Fragments arranged by size:
1844 1369 354
Bpu10I CC'TnA_GC
Cuts at: 0 535 1579 3567
Size: 535 1044 1988
Fragments arranged by size:
1988 1044 535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 763 3567
Size: 667 96 2804
Fragments arranged by size:
2804 667 96
BsaAI yAC'GTr
Cuts at: 0 840 3567
Size: 840 2727
BsaBI GATnn'nnATC
Cuts at: 0 1619 2672 3567
Size: 1619 1053 895
Fragments arranged by size:
1619 1053 895
BsaHI Gr'CG_yC
Cuts at: 0 1972 3567
Size: 1972 1595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 553 583 3567
Size: 272 30 251 30 2984
Fragments arranged by size:
2984 272 251 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 479 3567
Size: 479 3088
BseYI C'CCAG_C
Cuts at: 0 1155 2557 3567
Size: 1155 1402 1010
Fragments arranged by size:
1402 1155 1010
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 636 862 1036 3047 3414 3567
Size: 584 52 226 174 2011 367 153
Fragments arranged by size:
2011 584 367 226 174 153 52
BsiHKAI G_wGCw'C
Cuts at: 0 544 1650 3068 3567
Size: 544 1106 1418 499
Fragments arranged by size:
1418 1106 544 499
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1963 2975 3567
Size: 1963 1012 592
Fragments arranged by size:
1963 1012 592
Bsp1286I G_dGCh'C
Cuts at: 0 470 544 1650 3068 3567
Size: 470 74 1106 1418 499
Fragments arranged by size:
1418 1106 499 470 74
BspHI T'CATG_A
Cuts at: 0 1877 3567
Size: 1877 1690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2311 3567
Size: 2311 1256
BsrDI GCAATG_nn'
Cuts at: 0 2794 2946 3567
Size: 2794 152 621
Fragments arranged by size:
2794 621 152
BsrGI T'GTAC_A
Cuts at: 0 1890 2754 3567
Size: 1890 864 813
Fragments arranged by size:
1890 864 813
BstAPI GCAn_nnn'nTGC
Cuts at: 0 614 2014 2992 3567
Size: 614 1400 978 575
Fragments arranged by size:
1400 978 614 575
BstEII G'GTnAC_C
Cuts at: 0 807 1260 3567
Size: 807 453 2307
Fragments arranged by size:
2307 807 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 517 2111 3567
Size: 517 1594 1456
Fragments arranged by size:
1594 1456 517
Bsu36I CC'TnA_GG
Cuts at: 0 480 3567
Size: 480 3087
BtgI C'CryG_G
Cuts at: 0 788 3567
Size: 788 2779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 496 3567
Size: 496 3071
BtsI GCAGTG_nn'
Cuts at: 0 1182 2253 3567
Size: 1182 1071 1314
Fragments arranged by size:
1314 1182 1071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 659 694 3567
Size: 659 35 2873
Fragments arranged by size:
2873 659 35
DraI TTT'AAA
Cuts at: 0 2216 2456 2684 3395 3567
Size: 2216 240 228 711 172
Fragments arranged by size:
2216 711 240 228 172
DraIII CAC_nnn'GTG
Cuts at: 0 346 3567
Size: 346 3221
EaeI y'GGCC_r
Cuts at: 0 1548 3567
Size: 1548 2019
EarI CTCTTCn'nnn_
Cuts at: 0 475 497 582 1244 2781 3204 3481 3567
Size: 475 22 85 662 1537 423 277 86
Fragments arranged by size:
1537 662 475 423 277 86 85 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1412 1552 1724 1844 2198 2543 2762
Size: 839 573 140 172 120 354 345 219
Cuts at: 2762 3567
Size: 805
Fragments arranged by size:
839 805 573 354 345 219 172 140
120
EcoO109I rG'GnC_Cy
Cuts at: 0 484 3567
Size: 484 3083
EcoRI G'AATT_C
Cuts at: 0 441 814 3567
Size: 441 373 2753
Fragments arranged by size:
2753 441 373
EcoRV GAT'ATC
Cuts at: 0 2441 3567
Size: 2441 1126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2376 3567
Size: 2376 1191
HaeII r_GCGC'y
Cuts at: 0 3142 3567
Size: 3142 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 687 719 1755 1787 2157 2189
Size: 640 32 15 32 1036 32 370 32
Cuts at: 2189 2485 2517 3339 3371 3567
Size: 296 32 822 32 196
Fragments arranged by size:
1036 822 640 370 296 196 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 1239 1463 1534 2136 2915 2956 3567
Size: 1239 224 71 602 779 41 611
Fragments arranged by size:
1239 779 611 602 224 71 41
HindIII A'AGCT_T
Cuts at: 0 100 1555 3567
Size: 100 1455 2012
Fragments arranged by size:
2012 1455 100
HpaI GTT'AAC
Cuts at: 0 1463 3567
Size: 1463 2104
KpnI G_GTAC'C
Cuts at: 0 1677 3567
Size: 1677 1890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1380 1701 1772 1910 3567
Size: 1380 321 71 138 1657
Fragments arranged by size:
1657 1380 321 138 71
MslI CAynn'nnrTG
Cuts at: 0 515 1982 2109 3055 3567
Size: 515 1467 127 946 512
Fragments arranged by size:
1467 946 515 512 127
MspA1I CmG'CkG
Cuts at: 0 2782 2920 3567
Size: 2782 138 647
Fragments arranged by size:
2782 647 138
NcoI C'CATG_G
Cuts at: 0 788 3567
Size: 788 2779
NdeI CA'TA_TG
Cuts at: 0 906 3567
Size: 906 2661
NspI r_CATG'y
Cuts at: 0 581 1036 2377 2913 2990 3567
Size: 581 455 1341 536 77 577
Fragments arranged by size:
1341 581 577 536 455 77
PasI CC'CwG_GG
Cuts at: 0 826 3567
Size: 826 2741
PciI A'CATG_T
Cuts at: 0 1032 2373 3567
Size: 1032 1341 1194
Fragments arranged by size:
1341 1194 1032
PflMI CCAn_nnn'nTGG
Cuts at: 0 1630 2977 3567
Size: 1630 1347 590
Fragments arranged by size:
1630 1347 590
PfoI T'CCnGG_A
Cuts at: 0 129 2175 3567
Size: 129 2046 1392
Fragments arranged by size:
2046 1392 129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 868 900 3567
Size: 868 32 2667
Fragments arranged by size:
2667 868 32
PpuMI rG'GwC_Cy
Cuts at: 0 484 3567
Size: 484 3083
PstI C_TGCA'G
Cuts at: 0 2424 3567
Size: 2424 1143
PvuII CAG'CTG
Cuts at: 0 2782 2920 3567
Size: 2782 138 647
Fragments arranged by size:
2782 647 138
SalI G'TCGA_C
Cuts at: 0 1237 2954 3567
Size: 1237 1717 613
Fragments arranged by size:
1717 1237 613
SapI GCTCTTCn'nnn_
Cuts at: 0 475 3204 3567
Size: 475 2729 363
Fragments arranged by size:
2729 475 363
ScaI AGT'ACT
Cuts at: 0 1219 1588 3567
Size: 1219 369 1979
Fragments arranged by size:
1979 1219 369
SfcI C'TryA_G
Cuts at: 0 1271 2420 2964 3567
Size: 1271 1149 544 603
Fragments arranged by size:
1271 1149 603 544
SmlI C'TyrA_G
Cuts at: 0 646 778 3567
Size: 646 132 2789
Fragments arranged by size:
2789 646 132
SphI G_CATG'C
Cuts at: 0 2990 3567
Size: 2990 577
SspI AAT'ATT
Cuts at: 0 226 381 3567
Size: 226 155 3186
Fragments arranged by size:
3186 226 155
StuI AGG'CCT
Cuts at: 0 621 3567
Size: 621 2946
StyI C'CwwG_G
Cuts at: 0 788 2078 3567
Size: 788 1290 1489
Fragments arranged by size:
1489 1290 788
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 381 485 839 2071 3567
Size: 381 104 354 1232 1496
Fragments arranged by size:
1496 1232 381 354 104
TatI w'GTAC_w
Cuts at: 0 965 1217 1586 1713 1821 1890 2382 2754
Size: 965 252 369 127 108 69 492 372
Cuts at: 2754 3567
Size: 813
Fragments arranged by size:
965 813 492 372 369 252 127 108
69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 1654 1686 2622 2654 2890 2922
Size: 317 32 1305 32 936 32 236 32
Cuts at: 2922 3567
Size: 645
Fragments arranged by size:
1305 936 645 317 236 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1769 3567
Size: 1769 1798
XbaI T'CTAG_A
Cuts at: 0 2503 3567
Size: 2503 1064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 2076 3567
Size: 225 1851 1491
Fragments arranged by size:
1851 1491 225
XmnI GAAnn'nnTTC
Cuts at: 0 1067 3198 3467 3567
Size: 1067 2131 269 100
Fragments arranged by size:
2131 1067 269 100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApoI AseI BanI BanII BbsI BbvCI
BcgI BclI BglI BlpI BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI
MslI MspA1I NcoI NdeI NspI PasI PciI PflMI
PfoI PpiI PpuMI PstI PvuII SalI SapI ScaI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BfrBI
BglII Bme1580I BmtI BsaI BsaWI BsiEI BsiWI BsmI
BspEI BsrBI BsrFI BssHII BssSI BstBI BstYI BstZ17I
ClaI DrdI EagI EciI EcoICRI EcoNI FseI FspI
FspAI KasI MfeI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI XhoI XmaI ZraI