Restriction map for
pFN21AE4270
(Linear) (Six-base) MAPSORT of: KIEE4270.seq Check: 517 from: 1 to: 3375
KIEE4270.orf
With 182 enzymes: SgfI *
July 3, 2012 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2100 3375
Size: 2100 1275
Acc65I G'GTAC_C
Cuts at: 0 1728 3375
Size: 1728 1647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1705 2548 2802 3375
Size: 1400 305 843 254 573
Fragments arranged by size:
1400 843 573 305 254
AflIII A'CryG_T
Cuts at: 0 1250 1944 3375
Size: 1250 694 1431
Fragments arranged by size:
1431 1250 694
AhdI GACnn_n'nnGTC
Cuts at: 0 906 3375
Size: 906 2469
AleI CACnn'nnGTG
Cuts at: 0 2819 3375
Size: 2819 556
ApoI r'AATT_y
Cuts at: 0 814 1333 2368 3375
Size: 814 519 1035 1007
Fragments arranged by size:
1035 1007 814 519
AvaI C'yCGr_G
Cuts at: 0 1733 1787 3375
Size: 1733 54 1588
Fragments arranged by size:
1733 1588 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1966 1999 2176 2209 3375
Size: 1966 33 177 33 1166
Fragments arranged by size:
1966 1166 177 33 33
BamHI G'GATC_C
Cuts at: 0 1600 1782 3375
Size: 1600 182 1593
Fragments arranged by size:
1600 1593 182
BanI G'GyrC_C
Cuts at: 0 1728 2638 2697 3375
Size: 1728 910 59 678
Fragments arranged by size:
1728 910 678 59
BanII G_rGCy'C
Cuts at: 0 188 506 2712 3375
Size: 188 318 2206 663
Fragments arranged by size:
2206 663 318 188
BbsI GAAGACnn'nnnn_
Cuts at: 0 278 791 1374 2387 3375
Size: 278 513 583 1013 988
Fragments arranged by size:
1013 988 583 513 278
BbvCI CC'TCA_GC
Cuts at: 0 527 3200 3375
Size: 527 2673 175
Fragments arranged by size:
2673 527 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2087 2121 3375
Size: 2087 34 1254
Fragments arranged by size:
2087 1254 34
BglI GCCn_nnn'nGGC
Cuts at: 0 466 3375
Size: 466 2909
BglII A'GATC_T
Cuts at: 0 1689 2559 3375
Size: 1689 870 816
Fragments arranged by size:
1689 870 816
BlpI GC'TnA_GC
Cuts at: 0 337 3375
Size: 337 3038
BmrI ACTGGGnnnn_n'
Cuts at: 0 3356 3375
Size: 3356 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 488 3375
Size: 488 2887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 962 1006 1052 3375
Size: 496 466 44 46 2323
Fragments arranged by size:
2323 496 466 46 44
Bpu10I CC'TnA_GC
Cuts at: 0 227 236 527 3200 3375
Size: 227 9 291 2673 175
Fragments arranged by size:
2673 291 227 175 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1395 3018 3375
Size: 689 706 1623 357
Fragments arranged by size:
1623 706 689 357
BsaI GGTCTCn'nnnn_
Cuts at: 0 920 1028 2606 3375
Size: 920 108 1578 769
Fragments arranged by size:
1578 920 769 108
BsaAI yAC'GTr
Cuts at: 0 2950 3375
Size: 2950 425
BsaBI GATnn'nnATC
Cuts at: 0 1922 3375
Size: 1922 1453
BsaWI w'CCGG_w
Cuts at: 0 897 2361 3375
Size: 897 1464 1014
Fragments arranged by size:
1464 1014 897
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 682 712 1919 1949 2331 2361 3375
Size: 682 30 1207 30 382 30 1014
Fragments arranged by size:
1207 1014 682 382 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 365 1097 1100 1289 1805 1895 2156
Size: 178 187 732 3 189 516 90 261
Cuts at: 2156 2216 2219 3335 3375
Size: 60 3 1116 40
Fragments arranged by size:
1116 732 516 261 189 187 178 90
60 40 3 3
BseYI C'CCAG_C
Cuts at: 0 1038 1540 2234 2288 2395 2765 2942 3375
Size: 1038 502 694 54 107 370 177 433
Fragments arranged by size:
1038 694 502 433 370 177 107 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1463 3043 3375
Size: 883 580 1580 332
Fragments arranged by size:
1580 883 580 332
BsiHKAI G_wGCw'C
Cuts at: 0 188 228 506 1596 2712 3375
Size: 188 40 278 1090 1116 663
Fragments arranged by size:
1116 1090 663 278 188 40
Bsp1286I G_dGCh'C
Cuts at: 0 188 228 506 1596 2712 3375
Size: 188 40 278 1090 1116 663
Fragments arranged by size:
1116 1090 663 278 188 40
BspEI T'CCGG_A
Cuts at: 0 2361 3375
Size: 2361 1014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1622 1908 2100 3375
Size: 1622 286 192 1275
Fragments arranged by size:
1622 1275 286 192
BsrBI CCG'CTC
Cuts at: 0 336 3375
Size: 336 3039
BsrDI GCAATG_nn'
Cuts at: 0 205 599 3375
Size: 205 394 2776
Fragments arranged by size:
2776 394 205
BsrFI r'CCGG_y
Cuts at: 0 2216 3375
Size: 2216 1159
BsrGI T'GTAC_A
Cuts at: 0 6 1300 3375
Size: 6 1294 2075
Fragments arranged by size:
2075 1294 6
BssSI C'ACGA_G
Cuts at: 0 217 1286 3375
Size: 217 1069 2089
Fragments arranged by size:
2089 1069 217
BstAPI GCAn_nnn'nTGC
Cuts at: 0 119 146 3375
Size: 119 27 3229
Fragments arranged by size:
3229 119 27
BstBI TT'CG_AA
Cuts at: 0 75 3375
Size: 75 3300
BstEII G'GTnAC_C
Cuts at: 0 768 3375
Size: 768 2607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 583 1030 2950 3375
Size: 90 493 447 1920 425
Fragments arranged by size:
1920 493 447 425 90
BstYI r'GATC_y
Cuts at: 0 1449 1600 1689 1782 2559 3375
Size: 1449 151 89 93 777 816
Fragments arranged by size:
1449 816 777 151 93 89
Bsu36I CC'TnA_GG
Cuts at: 0 2702 3314 3375
Size: 2702 612 61
Fragments arranged by size:
2702 612 61
BtgI C'CryG_G
Cuts at: 0 694 2757 3375
Size: 694 2063 618
Fragments arranged by size:
2063 694 618
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2248 3375
Size: 2248 1127
BtsI GCAGTG_nn'
Cuts at: 0 348 1451 3231 3375
Size: 348 1103 1780 144
Fragments arranged by size:
1780 1103 348 144
DraI TTT'AAA
Cuts at: 0 611 3375
Size: 611 2764
DraIII CAC_nnn'GTG
Cuts at: 0 997 1858 3066 3375
Size: 997 861 1208 309
Fragments arranged by size:
1208 997 861 309
EaeI y'GGCC_r
Cuts at: 0 99 2013 2852 3375
Size: 99 1914 839 523
Fragments arranged by size:
1914 839 523 99
EarI CTCTTCn'nnn_
Cuts at: 0 543 795 2045 2565 3079 3375
Size: 543 252 1250 520 514 296
Fragments arranged by size:
1250 543 520 514 296 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1790 3375
Size: 1790 1585
EcoICRI GAG'CTC
Cuts at: 0 186 504 2710 3375
Size: 186 318 2206 665
Fragments arranged by size:
2206 665 318 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 962 1006 1052 1400 1705 2548 2802
Size: 496 466 44 46 348 305 843 254
Cuts at: 2802 3375
Size: 573
Fragments arranged by size:
843 573 496 466 348 305 254 46
44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3304 3317 3375
Size: 3304 13 58
Fragments arranged by size:
3304 58 13
EcoO109I rG'GnC_Cy
Cuts at: 0 231 3204 3309 3375
Size: 231 2973 105 66
Fragments arranged by size:
2973 231 105 66
HaeII r_GCGC'y
Cuts at: 0 1356 3375
Size: 1356 2019
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 772 804 892 924 1716 1748
Size: 19 32 721 32 88 32 792 32
Cuts at: 1748 2139 2171 2821 2853 3276 3308 3375
Size: 391 32 650 32 423 32 67
Fragments arranged by size:
792 721 650 423 391 88 67 32
32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 0 430 1393 3375
Size: 430 963 1982
Fragments arranged by size:
1982 963 430
HindIII A'AGCT_T
Cuts at: 0 406 605 3375
Size: 406 199 2770
Fragments arranged by size:
2770 406 199
KpnI G_GTAC'C
Cuts at: 0 1732 3375
Size: 1732 1643
MfeI C'AATT_G
Cuts at: 0 1013 2914 3375
Size: 1013 1901 461
Fragments arranged by size:
1901 1013 461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1899 2197 3375
Size: 1899 298 1178
Fragments arranged by size:
1899 1178 298
MscI TGG'CCA
Cuts at: 0 101 2854 3375
Size: 101 2753 521
Fragments arranged by size:
2753 521 101
MslI CAynn'nnrTG
Cuts at: 0 1251 1854 2009 2819 3375
Size: 1251 603 155 810 556
Fragments arranged by size:
1251 810 603 556 155
MspA1I CmG'CkG
Cuts at: 0 804 1128 1298 3375
Size: 804 324 170 2077
Fragments arranged by size:
2077 804 324 170
NaeI GCC'GGC
Cuts at: 0 2218 3375
Size: 2218 1157
NdeI CA'TA_TG
Cuts at: 0 947 2748 3375
Size: 947 1801 627
Fragments arranged by size:
1801 947 627
NgoMIV G'CCGG_C
Cuts at: 0 2216 3375
Size: 2216 1159
NspI r_CATG'y
Cuts at: 0 733 1254 3375
Size: 733 521 2121
Fragments arranged by size:
2121 733 521
PciI A'CATG_T
Cuts at: 0 1250 3375
Size: 1250 2125
PflMI CCAn_nnn'nTGG
Cuts at: 0 548 3375
Size: 548 2827
PfoI T'CCnGG_A
Cuts at: 0 1596 3375
Size: 1596 1779
PpuMI rG'GwC_Cy
Cuts at: 0 231 3204 3309 3375
Size: 231 2973 105 66
Fragments arranged by size:
2973 231 105 66
PshAI GACnn'nnGTC
Cuts at: 0 418 1094 3375
Size: 418 676 2281
Fragments arranged by size:
2281 676 418
PspXI vC'TCGA_Gb
Cuts at: 0 1733 1787 3375
Size: 1733 54 1588
Fragments arranged by size:
1733 1588 54
PstI C_TGCA'G
Cuts at: 0 215 2097 3375
Size: 215 1882 1278
Fragments arranged by size:
1882 1278 215
PvuII CAG'CTG
Cuts at: 0 804 1128 1298 3375
Size: 804 324 170 2077
Fragments arranged by size:
2077 804 324 170
SacI G_AGCT'C
Cuts at: 0 188 506 2712 3375
Size: 188 318 2206 663
Fragments arranged by size:
2206 663 318 188
SapI GCTCTTCn'nnn_
Cuts at: 0 2565 3375
Size: 2565 810
SexAI A'CCwGG_T
Cuts at: 0 3062 3375
Size: 3062 313
SfcI C'TryA_G
Cuts at: 0 211 2093 3375
Size: 211 1882 1282
Fragments arranged by size:
1882 1282 211
SmlI C'TyrA_G
Cuts at: 0 704 1374 1733 1787 3033 3375
Size: 704 670 359 54 1246 342
Fragments arranged by size:
1246 704 670 359 342 54
SpeI A'CTAG_T
Cuts at: 0 432 3375
Size: 432 2943
StyI C'CwwG_G
Cuts at: 0 582 1675 3375
Size: 582 1093 1700
Fragments arranged by size:
1700 1093 582
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 2630 3375
Size: 211 2419 745
Fragments arranged by size:
2419 745 211
TatI w'GTAC_w
Cuts at: 0 6 323 1300 3375
Size: 6 317 977 2075
Fragments arranged by size:
2075 977 317 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1919 1951 3100 3132 3375
Size: 1919 32 1149 32 243
Fragments arranged by size:
1919 1149 243 32 32
XbaI T'CTAG_A
Cuts at: 0 2250 3375
Size: 2250 1125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2493 3014 3375
Size: 2493 521 361
Fragments arranged by size:
2493 521 361
XhoI C'TCGA_G
Cuts at: 0 1733 1787 3375
Size: 1733 54 1588
Fragments arranged by size:
1733 1588 54
XmnI GAAnn'nnTTC
Cuts at: 0 71 1334 1625 3375
Size: 71 1263 291 1750
Fragments arranged by size:
1750 1263 291 71
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI ApoI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BglI
BglII BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI
PciI PflMI PfoI PpuMI PshAI PspXI PstI PvuII
SacI SapI SexAI SfcI SmlI SpeI StyI TaqII
TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BbeI
BciVI BclI BfrBI Bme1580I BmgBI BmtI BsaHI BsiEI
BsiWI BsmI BsmBI BspHI BssHII BstZ17I ClaI CspCI
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KasI MluI NarI NcoI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI Tth111I XmaI ZraI