Restriction map for
pFN21AE4269
(Linear) (Six-base) MAPSORT of: KIEE4269.seq Check: 5949 from: 1 to: 3387
KIEE4269.orf
With 182 enzymes: SgfI *
March 20, 2014 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1123 3387
Size: 1123 2264
Acc65I G'GTAC_C
Cuts at: 0 986 3387
Size: 986 2401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 3076 3143 3387
Size: 196 2880 67 244
Fragments arranged by size:
2880 244 196 67
AfeI AGC'GCT
Cuts at: 0 2194 3387
Size: 2194 1193
AflIII A'CryG_T
Cuts at: 0 2097 3387
Size: 2097 1290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1023 1055 1653 1685 1901 1933 3387
Size: 1023 32 598 32 216 32 1454
Fragments arranged by size:
1454 1023 598 216 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 601 3387
Size: 601 2786
ApaI G_GGCC'C
Cuts at: 0 2546 3387
Size: 2546 841
ApoI r'AATT_y
Cuts at: 0 1563 3387
Size: 1563 1824
AvrII C'CTAG_G
Cuts at: 0 2383 3387
Size: 2383 1004
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3149 3182 3387
Size: 3149 33 205
Fragments arranged by size:
3149 205 33
BamHI G'GATC_C
Cuts at: 0 1010 3387
Size: 1010 2377
BanI G'GyrC_C
Cuts at: 0 343 632 986 3387
Size: 343 289 354 2401
Fragments arranged by size:
2401 354 343 289
BanII G_rGCy'C
Cuts at: 0 2546 3257 3387
Size: 2546 711 130
Fragments arranged by size:
2546 711 130
BbeI G_GCGC'C
Cuts at: 0 636 3387
Size: 636 2751
BbsI GAAGACnn'nnnn_
Cuts at: 0 1145 1310 1669 3131 3223 3387
Size: 1145 165 359 1462 92 164
Fragments arranged by size:
1462 1145 359 165 164 92
BciVI GTATCCnnnnn_n'
Cuts at: 0 925 3387
Size: 925 2462
BfrBI ATG'CAT
Cuts at: 0 942 3387
Size: 942 2445
BglII A'GATC_T
Cuts at: 0 4 71 480 2058 2318 3387
Size: 4 67 409 1578 260 1069
Fragments arranged by size:
1578 1069 409 260 67 4
BlpI GC'TnA_GC
Cuts at: 0 3032 3387
Size: 3032 355
Bme1580I G_kGCm'C
Cuts at: 0 2546 3387
Size: 2546 841
BmgBI CAC'GTC
Cuts at: 0 128 2100 3387
Size: 128 1972 1287
Fragments arranged by size:
1972 1287 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 2043 3387
Size: 2043 1344
BmtI G_CTAG'C
Cuts at: 0 2998 3387
Size: 2998 389
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2498 3387
Size: 2498 889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 199 391 934 2027 2211 2828 3032
Size: 9 190 192 543 1093 184 617 204
Cuts at: 3032 3387
Size: 355
Fragments arranged by size:
1093 617 543 355 204 192 190 184
9
Bpu10I CC'TnA_GC
Cuts at: 0 1969 2870 3387
Size: 1969 901 517
Fragments arranged by size:
1969 901 517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 1723 3387
Size: 469 1254 1664
Fragments arranged by size:
1664 1254 469
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 452 2804 2981 3387
Size: 91 361 2352 177 406
Fragments arranged by size:
2352 406 361 177 91
BsaAI yAC'GTr
Cuts at: 0 2106 3387
Size: 2106 1281
BsaBI GATnn'nnATC
Cuts at: 0 2156 3387
Size: 2156 1231
BsaHI Gr'CG_yC
Cuts at: 0 633 3101 3160 3387
Size: 633 2468 59 227
Fragments arranged by size:
2468 633 227 59
BsaWI w'CCGG_w
Cuts at: 0 1013 3387
Size: 1013 2374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1023 1053 2352 2382 3387
Size: 1023 30 1299 30 1005
Fragments arranged by size:
1299 1023 1005 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 1044 2149 2214 2471 3387
Size: 196 848 1105 65 257 916
Fragments arranged by size:
1105 916 848 257 196 65
BseYI C'CCAG_C
Cuts at: 0 1570 2524 2630 2901 2982 3387
Size: 1570 954 106 271 81 405
Fragments arranged by size:
1570 954 405 271 106 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 845 3109 3387
Size: 133 712 2264 278
Fragments arranged by size:
2264 712 278 133
BsiHKAI G_wGCw'C
Cuts at: 0 560 2644 3257 3387
Size: 560 2084 613 130
Fragments arranged by size:
2084 613 560 130
BsmI GAATG_Cn'
Cuts at: 0 1469 3387
Size: 1469 1918
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3092 3151 3387
Size: 3092 59 236
Fragments arranged by size:
3092 236 59
Bsp1286I G_dGCh'C
Cuts at: 0 560 2546 2644 3257 3387
Size: 560 1986 98 613 130
Fragments arranged by size:
1986 613 560 130 98
BspEI T'CCGG_A
Cuts at: 0 1013 3387
Size: 1013 2374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 998 1123 2822 2918 2972 2999 3387
Size: 998 125 1699 96 54 27 388
Fragments arranged by size:
1699 998 388 125 96 54 27
BsrFI r'CCGG_y
Cuts at: 0 920 3387
Size: 920 2467
BsrGI T'GTAC_A
Cuts at: 0 264 1869 1937 3387
Size: 264 1605 68 1450
Fragments arranged by size:
1605 1450 264 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2659 3387
Size: 2659 728
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2835 3387
Size: 2835 552
BstYI r'GATC_y
Cuts at: 0 4 71 480 702 1010 2058 2318 3072
Size: 4 67 409 222 308 1048 260 754
Cuts at: 3072 3387
Size: 315
Fragments arranged by size:
1048 754 409 315 308 260 222 67
4
BtgI C'CryG_G
Cuts at: 0 1259 1269 2360 3387
Size: 1259 10 1091 1027
Fragments arranged by size:
1259 1091 1027 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 2165 3387
Size: 75 2090 1222
Fragments arranged by size:
2090 1222 75
BtsI GCAGTG_nn'
Cuts at: 0 388 978 1628 1774 2254 3169 3387
Size: 388 590 650 146 480 915 218
Fragments arranged by size:
915 650 590 480 388 218 146
DraIII CAC_nnn'GTG
Cuts at: 0 426 3387
Size: 426 2961
EaeI y'GGCC_r
Cuts at: 0 1517 3318 3387
Size: 1517 1801 69
Fragments arranged by size:
1801 1517 69
EarI CTCTTCn'nnn_
Cuts at: 0 893 1241 2467 2598 3290 3387
Size: 893 348 1226 131 692 97
Fragments arranged by size:
1226 893 692 348 131 97
EcoICRI GAG'CTC
Cuts at: 0 3255 3387
Size: 3255 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 196 199 391 934 2027 2211 2828
Size: 9 187 3 192 543 1093 184 617
Cuts at: 2828 3032 3076 3143 3387
Size: 204 44 67 244
Fragments arranged by size:
1093 617 543 244 204 192 187 184
67 44 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2873 3387
Size: 2873 514
EcoO109I rG'GnC_Cy
Cuts at: 0 1251 2543 2907 2961 3387
Size: 1251 1292 364 54 426
Fragments arranged by size:
1292 1251 426 364 54
EcoRV GAT'ATC
Cuts at: 0 1163 1223 3387
Size: 1163 60 2164
Fragments arranged by size:
2164 1163 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2080 3369 3387
Size: 2080 1289 18
Fragments arranged by size:
2080 1289 18
HaeII r_GCGC'y
Cuts at: 0 636 2196 3387
Size: 636 1560 1191
Fragments arranged by size:
1560 1191 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 100 132 1132 1147 1164 1179
Size: 58 32 10 32 1000 15 17 15
Cuts at: 1179 1357 1389 1828 1860 2611 2643 2710 2742
Size: 178 32 439 32 751 32 67 32
Cuts at: 2742 3089 3121 3387
Size: 347 32 266
Fragments arranged by size:
1000 751 439 347 266 178 67 58
32 32 32 32 32 32 32 17
15 15 10
HincII GTy'rAC
Cuts at: 0 1860 1998 3387
Size: 1860 138 1389
Fragments arranged by size:
1860 1389 138
HindIII A'AGCT_T
Cuts at: 0 1960 3387
Size: 1960 1427
HpaI GTT'AAC
Cuts at: 0 1998 3387
Size: 1998 1389
KasI G'GCGC_C
Cuts at: 0 632 3387
Size: 632 2755
KpnI G_GTAC'C
Cuts at: 0 990 3387
Size: 990 2397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2428 2540 3155 3387
Size: 2428 112 615 232
Fragments arranged by size:
2428 615 232 112
MscI TGG'CCA
Cuts at: 0 1519 3320 3387
Size: 1519 1801 67
Fragments arranged by size:
1801 1519 67
MspA1I CmG'CkG
Cuts at: 0 772 1338 2289 2901 2982 3387
Size: 772 566 951 612 81 405
Fragments arranged by size:
951 772 612 566 405 81
NaeI GCC'GGC
Cuts at: 0 922 3387
Size: 922 2465
NarI GG'CG_CC
Cuts at: 0 633 3387
Size: 633 2754
NcoI C'CATG_G
Cuts at: 0 1259 3387
Size: 1259 2128
NgoMIV G'CCGG_C
Cuts at: 0 920 3387
Size: 920 2467
NheI G'CTAG_C
Cuts at: 0 2994 3387
Size: 2994 393
NsiI A_TGCA'T
Cuts at: 0 944 3387
Size: 944 2443
NspI r_CATG'y
Cuts at: 0 942 1075 3387
Size: 942 133 2312
Fragments arranged by size:
2312 942 133
PasI CC'CwG_GG
Cuts at: 0 1345 1905 3387
Size: 1345 560 1482
Fragments arranged by size:
1482 1345 560
PflMI CCAn_nnn'nTGG
Cuts at: 0 2806 3387
Size: 2806 581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1023 1055 2631 2663 3387
Size: 1023 32 1576 32 724
Fragments arranged by size:
1576 1023 724 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2907 2961 3387
Size: 2907 54 426
Fragments arranged by size:
2907 426 54
PsiI TTA'TAA
Cuts at: 0 721 3387
Size: 721 2666
PspOMI G'GGCC_C
Cuts at: 0 2542 3387
Size: 2542 845
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1413 1445 3387
Size: 1413 32 1942
Fragments arranged by size:
1942 1413 32
PstI C_TGCA'G
Cuts at: 0 160 713 1836 3387
Size: 160 553 1123 1551
Fragments arranged by size:
1551 1123 553 160
PvuII CAG'CTG
Cuts at: 0 772 2901 2982 3387
Size: 772 2129 81 405
Fragments arranged by size:
2129 772 405 81
SacI G_AGCT'C
Cuts at: 0 3257 3387
Size: 3257 130
SfcI C'TryA_G
Cuts at: 0 156 709 1832 1929 3387
Size: 156 553 1123 97 1458
Fragments arranged by size:
1458 1123 553 156 97
SfoI GGC'GCC
Cuts at: 0 634 3387
Size: 634 2753
SmlI C'TyrA_G
Cuts at: 0 484 1702 3387
Size: 484 1218 1685
Fragments arranged by size:
1685 1218 484
SphI G_CATG'C
Cuts at: 0 942 3387
Size: 942 2445
StuI AGG'CCT
Cuts at: 0 2478 3387
Size: 2478 909
StyI C'CwwG_G
Cuts at: 0 339 437 1259 2275 2383 2566 2664 3026
Size: 339 98 822 1016 108 183 98 362
Cuts at: 3026 3387
Size: 361
Fragments arranged by size:
1016 822 362 361 339 183 108 98
98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2214 2452 2931 3039 3080 3387
Size: 2214 238 479 108 41 307
Fragments arranged by size:
2214 479 307 238 108 41
TatI w'GTAC_w
Cuts at: 0 264 788 1869 1937 3387
Size: 264 524 1081 68 1450
Fragments arranged by size:
1450 1081 524 264 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2352 2384 3292 3324 3387
Size: 2352 32 908 32 63
Fragments arranged by size:
2352 908 63 32 32
XbaI T'CTAG_A
Cuts at: 0 189 3387
Size: 189 3198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 696 2077 2446 2898 2979 3387
Size: 696 1381 369 452 81 408
Fragments arranged by size:
1381 696 452 408 369 81
XmnI GAAnn'nnTTC
Cuts at: 0 2759 3387
Size: 2759 628
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AloI AlwNI ApaI
ApoI AvrII BaeI BamHI BanI BanII BbeI BbsI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MmeI MscI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PflMI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuII SacI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI AvaI BbvCI BcgI BclI
BglI BsiEI BsiWI BspHI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoRI FseI FspI FspAI MfeI MluI
MslI NdeI NotI NruI PacI PciI PfoI PmeI
PmlI PshAI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmaI
ZraI