Restriction map for
pFN21AE4264
(Linear) (Six-base) MAPSORT of: KIEE4264.seq Check: 1636 from: 1 to: 3207
KIEE4264.orf
With 182 enzymes: SgfI *
July 3, 2012 18:53 ..
AccI GT'mk_AC
Cuts at: 0 2532 3207
Size: 2532 675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 410 596 711 1311 1460 1724 2009
Size: 23 387 186 115 600 149 264 285
Cuts at: 2009 3207
Size: 1198
Fragments arranged by size:
1198 600 387 285 264 186 149 115
23
AflII C'TTAA_G
Cuts at: 0 501 519 3207
Size: 501 18 2688
Fragments arranged by size:
2688 501 18
AflIII A'CryG_T
Cuts at: 0 735 2162 3207
Size: 735 1427 1045
Fragments arranged by size:
1427 1045 735
AleI CACnn'nnGTG
Cuts at: 0 412 3207
Size: 412 2795
AlwNI CAG_nnn'CTG
Cuts at: 0 177 835 2773 3207
Size: 177 658 1938 434
Fragments arranged by size:
1938 658 434 177
ApoI r'AATT_y
Cuts at: 0 1350 1607 2080 2203 2882 2920 3207
Size: 1350 257 473 123 679 38 287
Fragments arranged by size:
1350 679 473 287 257 123 38
AvaI C'yCGr_G
Cuts at: 0 1820 3207
Size: 1820 1387
BamHI G'GATC_C
Cuts at: 0 207 3207
Size: 207 3000
BanI G'GyrC_C
Cuts at: 0 2332 2912 3207
Size: 2332 580 295
Fragments arranged by size:
2332 580 295
BanII G_rGCy'C
Cuts at: 0 341 725 2039 3166 3207
Size: 341 384 1314 1127 41
Fragments arranged by size:
1314 1127 384 341 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 732 1036 2214 2501 3145 3207
Size: 732 304 1178 287 644 62
Fragments arranged by size:
1178 732 644 304 287 62
BbvCI CC'TCA_GC
Cuts at: 0 2630 3207
Size: 2630 577
BciVI GTATCCnnnnn_n'
Cuts at: 0 767 1596 3207
Size: 767 829 1611
Fragments arranged by size:
1611 829 767
BmgBI CAC'GTC
Cuts at: 0 738 3143 3207
Size: 738 2405 64
Fragments arranged by size:
2405 738 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 2169 3207
Size: 2169 1038
BmtI G_CTAG'C
Cuts at: 0 193 1738 3207
Size: 193 1545 1469
Fragments arranged by size:
1545 1469 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1511 3040 3207
Size: 1297 214 1529 167
Fragments arranged by size:
1529 1297 214 167
Bpu10I CC'TnA_GC
Cuts at: 0 2038 2630 3207
Size: 2038 592 577
Fragments arranged by size:
2038 592 577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 2992 3207
Size: 130 2862 215
Fragments arranged by size:
2862 215 130
BsaI GGTCTCn'nnnn_
Cuts at: 0 1656 3207
Size: 1656 1551
BsaBI GATnn'nnATC
Cuts at: 0 514 988 3207
Size: 514 474 2219
Fragments arranged by size:
2219 514 474
BsaHI Gr'CG_yC
Cuts at: 0 2529 3207
Size: 2529 678
BsaWI w'CCGG_w
Cuts at: 0 743 3207
Size: 743 2464
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3058 3088 3207
Size: 3058 30 119
Fragments arranged by size:
3058 119 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1467 1514 1730 1886 3207
Size: 1467 47 216 156 1321
Fragments arranged by size:
1467 1321 216 156 47
BseYI C'CCAG_C
Cuts at: 0 1842 2486 3207
Size: 1842 644 721
Fragments arranged by size:
1842 721 644
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 944 1018 1619 3145 3207
Size: 824 120 74 601 1526 62
Fragments arranged by size:
1526 824 601 120 74 62
BsiHKAI G_wGCw'C
Cuts at: 0 725 1973 3166 3207
Size: 725 1248 1193 41
Fragments arranged by size:
1248 1193 725 41
BsmI GAATG_Cn'
Cuts at: 0 1778 1999 3207
Size: 1778 221 1208
Fragments arranged by size:
1778 1208 221
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2995 3207
Size: 2995 212
Bsp1286I G_dGCh'C
Cuts at: 0 341 725 1973 2039 3166 3207
Size: 341 384 1248 66 1127 41
Fragments arranged by size:
1248 1127 384 341 66 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2246 2318 3207
Size: 2246 72 889
Fragments arranged by size:
2246 889 72
BsrBI CCG'CTC
Cuts at: 0 2375 2517 2910 3207
Size: 2375 142 393 297
Fragments arranged by size:
2375 393 297 142
BsrDI GCAATG_nn'
Cuts at: 0 2073 3207
Size: 2073 1134
BsrGI T'GTAC_A
Cuts at: 0 2126 3207
Size: 2126 1081
BstBI TT'CG_AA
Cuts at: 0 2063 2299 3207
Size: 2063 236 908
Fragments arranged by size:
2063 908 236
BstEII G'GTnAC_C
Cuts at: 0 2589 3207
Size: 2589 618
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2868 3207
Size: 2868 339
BstYI r'GATC_y
Cuts at: 0 207 597 1573 3207
Size: 207 390 976 1634
Fragments arranged by size:
1634 976 390 207
BtsI GCAGTG_nn'
Cuts at: 0 791 833 2195 3207
Size: 791 42 1362 1012
Fragments arranged by size:
1362 1012 791 42
DraI TTT'AAA
Cuts at: 0 957 3207
Size: 957 2250
EaeI y'GGCC_r
Cuts at: 0 416 3207
Size: 416 2791
EarI CTCTTCn'nnn_
Cuts at: 0 42 652 730 1535 1715 1922 2693 2714
Size: 42 610 78 805 180 207 771 21
Cuts at: 2714 2987 3207
Size: 273 220
Fragments arranged by size:
805 771 610 273 220 207 180 78
42 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 790 3207
Size: 790 2417
EcoICRI GAG'CTC
Cuts at: 0 723 3164 3207
Size: 723 2441 43
Fragments arranged by size:
2441 723 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 410 596 711 1297 1311 1460 1511
Size: 23 387 186 115 586 14 149 51
Cuts at: 1511 1724 2009 3040 3207
Size: 213 285 1031 167
Fragments arranged by size:
1031 586 387 285 213 186 167 149
115 51 23 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 377 2104 3118 3207
Size: 377 1727 1014 89
Fragments arranged by size:
1727 1014 377 89
EcoO109I rG'GnC_Cy
Cuts at: 0 2616 3207
Size: 2616 591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2076 3207
Size: 2076 1131
HaeII r_GCGC'y
Cuts at: 0 2100 3207
Size: 2100 1107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 91 123 195 227 1087 1114
Size: 34 32 25 32 72 32 860 27
Cuts at: 1114 1119 1140 1146 1172 1506 1538 1706 1738
Size: 5 21 6 26 334 32 168 32
Cuts at: 1738 2808 2840 3207
Size: 1070 32 367
Fragments arranged by size:
1070 860 367 334 168 72 34 32
32 32 32 32 32 27 26 25
21 6 5
HincII GTy'rAC
Cuts at: 0 2318 3207
Size: 2318 889
HindIII A'AGCT_T
Cuts at: 0 392 3207
Size: 392 2815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2072 2534 3207
Size: 2072 462 673
Fragments arranged by size:
2072 673 462
MslI CAynn'nnrTG
Cuts at: 0 412 713 994 1751 2165 3207
Size: 412 301 281 757 414 1042
Fragments arranged by size:
1042 757 414 412 301 281
MspA1I CmG'CkG
Cuts at: 0 177 1228 1731 3018 3060 3207
Size: 177 1051 503 1287 42 147
Fragments arranged by size:
1287 1051 503 177 147 42
NdeI CA'TA_TG
Cuts at: 0 1982 3207
Size: 1982 1225
NheI G'CTAG_C
Cuts at: 0 189 1734 3207
Size: 189 1545 1473
Fragments arranged by size:
1545 1473 189
NspI r_CATG'y
Cuts at: 0 1086 2166 3207
Size: 1086 1080 1041
Fragments arranged by size:
1086 1080 1041
PciI A'CATG_T
Cuts at: 0 2162 3207
Size: 2162 1045
PflMI CCAn_nnn'nTGG
Cuts at: 0 1495 2139 2586 3063 3207
Size: 1495 644 447 477 144
Fragments arranged by size:
1495 644 477 447 144
PfoI T'CCnGG_A
Cuts at: 0 1056 1274 2138 3207
Size: 1056 218 864 1069
Fragments arranged by size:
1069 1056 864 218
PpuMI rG'GwC_Cy
Cuts at: 0 2616 3207
Size: 2616 591
PshAI GACnn'nnGTC
Cuts at: 0 1285 3207
Size: 1285 1922
PvuII CAG'CTG
Cuts at: 0 177 1228 1731 3018 3060 3207
Size: 177 1051 503 1287 42 147
Fragments arranged by size:
1287 1051 503 177 147 42
SacI G_AGCT'C
Cuts at: 0 725 3166 3207
Size: 725 2441 41
Fragments arranged by size:
2441 725 41
SapI GCTCTTCn'nnn_
Cuts at: 0 730 1715 3207
Size: 730 985 1492
Fragments arranged by size:
1492 985 730
ScaI AGT'ACT
Cuts at: 0 1341 3207
Size: 1341 1866
SexAI A'CCwGG_T
Cuts at: 0 1760 3207
Size: 1760 1447
SfcI C'TryA_G
Cuts at: 0 1229 1463 3207
Size: 1229 234 1744
Fragments arranged by size:
1744 1229 234
SmaI CCC'GGG
Cuts at: 0 1822 3207
Size: 1822 1385
SmlI C'TyrA_G
Cuts at: 0 145 501 519 3007 3207
Size: 145 356 18 2488 200
Fragments arranged by size:
2488 356 200 145 18
StyI C'CwwG_G
Cuts at: 0 966 2280 2963 3207
Size: 966 1314 683 244
Fragments arranged by size:
1314 966 683 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1680 3207
Size: 1680 1527
TatI w'GTAC_w
Cuts at: 0 198 1251 1339 2126 3207
Size: 198 1053 88 787 1081
Fragments arranged by size:
1081 1053 787 198 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 150 182 934 966 3058 3090 3207
Size: 150 32 752 32 2092 32 117
Fragments arranged by size:
2092 752 150 117 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 3057 3207
Size: 489 2568 150
Fragments arranged by size:
2568 489 150
XmaI C'CCGG_G
Cuts at: 0 1820 3207
Size: 1820 1387
XmnI GAAnn'nnTTC
Cuts at: 0 1607 1709 2114 3207
Size: 1607 102 405 1093
Fragments arranged by size:
1607 1093 405 102
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BciVI BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrGI BstBI BstEII BstXI BstYI
BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII MmeI MslI
MspA1I NdeI NheI NspI PciI PflMI PfoI PpuMI
PshAI PvuII SacI SapI ScaI SexAI SfcI SmaI
SmlI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BplI BsaAI BsiEI BsiWI BspEI BspHI BsrFI BssHII
BssSI BstAPI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI ZraI