Restriction map for
pFN21AE4261
(Linear) (Six-base) MAPSORT of: KIEE4261.seq Check: 62 from: 1 to: 3171
KIEE4261.orf
With 182 enzymes: SgfI *
March 22, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1431 3171
Size: 1431 1740
AccI GT'mk_AC
Cuts at: 0 1056 3171
Size: 1056 2115
Acc65I G'GTAC_C
Cuts at: 0 1034 1354 3171
Size: 1034 320 1817
Fragments arranged by size:
1817 1034 320
AclI AA'CG_TT
Cuts at: 0 1285 3171
Size: 1285 1886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1152 3144 3171
Size: 1094 58 1992 27
Fragments arranged by size:
1992 1094 58 27
AfeI AGC'GCT
Cuts at: 0 293 3171
Size: 293 2878
AflII C'TTAA_G
Cuts at: 0 550 3171
Size: 550 2621
AflIII A'CryG_T
Cuts at: 0 169 3171
Size: 169 3002
AgeI A'CCGG_T
Cuts at: 0 985 3171
Size: 985 2186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 251 935 3171
Size: 251 684 2236
Fragments arranged by size:
2236 684 251
AlwNI CAG_nnn'CTG
Cuts at: 0 2544 2961 3171
Size: 2544 417 210
Fragments arranged by size:
2544 417 210
ApoI r'AATT_y
Cuts at: 0 944 1097 1751 1843 2266 2909 3171
Size: 944 153 654 92 423 643 262
Fragments arranged by size:
944 654 643 423 262 153 92
BamHI G'GATC_C
Cuts at: 0 2298 3171
Size: 2298 873
BanI G'GyrC_C
Cuts at: 0 1034 1354 1614 3171
Size: 1034 320 260 1557
Fragments arranged by size:
1557 1034 320 260
BanII G_rGCy'C
Cuts at: 0 74 600 3171
Size: 74 526 2571
Fragments arranged by size:
2571 526 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 465 2375 3171
Size: 465 1910 796
Fragments arranged by size:
1910 796 465
BclI T'GATC_A
Cuts at: 0 1338 1419 2058 3144 3171
Size: 1338 81 639 1086 27
Fragments arranged by size:
1338 1086 639 81 27
BfrBI ATG'CAT
Cuts at: 0 19 371 3171
Size: 19 352 2800
Fragments arranged by size:
2800 352 19
BglII A'GATC_T
Cuts at: 0 718 1193 3171
Size: 718 475 1978
Fragments arranged by size:
1978 718 475
BlpI GC'TnA_GC
Cuts at: 0 1231 2409 3171
Size: 1231 1178 762
Fragments arranged by size:
1231 1178 762
BmrI ACTGGGnnnn_n'
Cuts at: 0 1908 2092 3052 3171
Size: 1908 184 960 119
Fragments arranged by size:
1908 960 184 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 824 3171
Size: 644 180 2347
Fragments arranged by size:
2347 644 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 2197 3171
Size: 1654 543 974
Fragments arranged by size:
1654 974 543
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 3171
Size: 331 2840
BsaWI w'CCGG_w
Cuts at: 0 985 3171
Size: 985 2186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2765 2795 3171
Size: 2765 30 376
Fragments arranged by size:
2765 376 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 608 2073 3061 3171
Size: 608 1465 988 110
Fragments arranged by size:
1465 988 608 110
BseYI C'CCAG_C
Cuts at: 0 8 978 3171
Size: 8 970 2193
Fragments arranged by size:
2193 970 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2669 3171
Size: 2669 502
BsmI GAATG_Cn'
Cuts at: 0 373 1386 2987 3171
Size: 373 1013 1601 184
Fragments arranged by size:
1601 1013 373 184
Bsp1286I G_dGCh'C
Cuts at: 0 74 600 3171
Size: 74 526 2571
Fragments arranged by size:
2571 526 74
BspHI T'CATG_A
Cuts at: 0 201 840 2282 3171
Size: 201 639 1442 889
Fragments arranged by size:
1442 889 639 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 1431 3171
Size: 219 1212 1740
Fragments arranged by size:
1740 1212 219
BsrDI GCAATG_nn'
Cuts at: 0 1338 2171 2822 3171
Size: 1338 833 651 349
Fragments arranged by size:
1338 833 651 349
BsrFI r'CCGG_y
Cuts at: 0 985 3171
Size: 985 2186
BsrGI T'GTAC_A
Cuts at: 0 164 417 1975 3171
Size: 164 253 1558 1196
Fragments arranged by size:
1558 1196 253 164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1150 2392 3171
Size: 1150 1242 779
Fragments arranged by size:
1242 1150 779
BstYI r'GATC_y
Cuts at: 0 718 1193 2298 3087 3171
Size: 718 475 1105 789 84
Fragments arranged by size:
1105 789 718 475 84
Bsu36I CC'TnA_GG
Cuts at: 0 2176 3171
Size: 2176 995
BtgI C'CryG_G
Cuts at: 0 1935 3171
Size: 1935 1236
BtsI GCAGTG_nn'
Cuts at: 0 1057 3171
Size: 1057 2114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 762 797 3171
Size: 762 35 2374
Fragments arranged by size:
2374 762 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 197 3171
Size: 197 2974
EaeI y'GGCC_r
Cuts at: 0 498 3171
Size: 498 2673
EarI CTCTTCn'nnn_
Cuts at: 0 3001 3171
Size: 3001 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 824 1094 1152 3144 3171
Size: 644 180 270 58 1992 27
Fragments arranged by size:
1992 644 270 180 58 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 935 1609 3171
Size: 935 674 1562
Fragments arranged by size:
1562 935 674
EcoRV GAT'ATC
Cuts at: 0 408 1433 1959 3171
Size: 408 1025 526 1212
Fragments arranged by size:
1212 1025 526 408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2505 3171
Size: 2505 666
FspI TGC'GCA
Cuts at: 0 2260 3171
Size: 2260 911
HaeII r_GCGC'y
Cuts at: 0 295 3171
Size: 295 2876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 1503 1535 2765 2797 3171
Size: 110 32 1361 32 1230 32 374
Fragments arranged by size:
1361 1230 374 110 32 32 32
HincII GTy'rAC
Cuts at: 0 1932 2664 3171
Size: 1932 732 507
Fragments arranged by size:
1932 732 507
HindIII A'AGCT_T
Cuts at: 0 1119 3171
Size: 1119 2052
HpaI GTT'AAC
Cuts at: 0 2664 3171
Size: 2664 507
KpnI G_GTAC'C
Cuts at: 0 1038 1358 3171
Size: 1038 320 1813
Fragments arranged by size:
1813 1038 320
MfeI C'AATT_G
Cuts at: 0 502 3171
Size: 502 2669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1580 1929 2208 3171
Size: 1207 373 349 279 963
Fragments arranged by size:
1207 963 373 349 279
MscI TGG'CCA
Cuts at: 0 500 3171
Size: 500 2671
MslI CAynn'nnrTG
Cuts at: 0 174 3171
Size: 174 2997
MspA1I CmG'CkG
Cuts at: 0 546 978 2659 3171
Size: 546 432 1681 512
Fragments arranged by size:
1681 546 512 432
NcoI C'CATG_G
Cuts at: 0 1935 3171
Size: 1935 1236
NsiI A_TGCA'T
Cuts at: 0 21 373 3171
Size: 21 352 2798
Fragments arranged by size:
2798 352 21
NspI r_CATG'y
Cuts at: 0 173 2316 2535 3171
Size: 173 2143 219 636
Fragments arranged by size:
2143 636 219 173
PciI A'CATG_T
Cuts at: 0 169 3171
Size: 169 3002
PsiI TTA'TAA
Cuts at: 0 94 2420 3171
Size: 94 2326 751
Fragments arranged by size:
2326 751 94
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2182 2214 3171
Size: 2182 32 957
Fragments arranged by size:
2182 957 32
PvuII CAG'CTG
Cuts at: 0 546 978 2659 3171
Size: 546 432 1681 512
Fragments arranged by size:
1681 546 512 432
ScaI AGT'ACT
Cuts at: 0 1628 1649 3171
Size: 1628 21 1522
Fragments arranged by size:
1628 1522 21
SfcI C'TryA_G
Cuts at: 0 1344 3171
Size: 1344 1827
SmlI C'TyrA_G
Cuts at: 0 550 1669 2212 3171
Size: 550 1119 543 959
Fragments arranged by size:
1119 959 550 543
SpeI A'CTAG_T
Cuts at: 0 3018 3171
Size: 3018 153
SphI G_CATG'C
Cuts at: 0 2535 3171
Size: 2535 636
SspI AAT'ATT
Cuts at: 0 255 390 1081 2151 2884 3171
Size: 255 135 691 1070 733 287
Fragments arranged by size:
1070 733 691 287 255 135
StuI AGG'CCT
Cuts at: 0 1915 3171
Size: 1915 1256
StyI C'CwwG_G
Cuts at: 0 773 853 1935 2489 3171
Size: 773 80 1082 554 682
Fragments arranged by size:
1082 773 682 554 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 2041 3171
Size: 31 2010 1130
Fragments arranged by size:
2010 1130 31
TatI w'GTAC_w
Cuts at: 0 63 164 417 609 1626 1647 1860 1975
Size: 63 101 253 192 1017 21 213 115
Cuts at: 1975 3171
Size: 1196
Fragments arranged by size:
1196 1017 253 213 192 115 101 63
21
XmnI GAAnn'nnTTC
Cuts at: 0 987 1287 2924 3171
Size: 987 300 1637 247
Fragments arranged by size:
1637 987 300 247
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApoI BamHI BanI BanII BbsI
BclI BfrBI BglII BlpI BmrI BpmI BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI
BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PciI PsiI PsrI PvuII ScaI SfcI SmlI SpeI
SphI SspI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BglI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI
DraIII EagI EciI EcoICRI EcoO109I EcoRI FseI FspAI
KasI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI