Restriction map for
pFN21AE4260
(Linear) (Six-base) MAPSORT of: KIEE4260.seq Check: 7181 from: 1 to: 3159
KIEE4260.orf
With 182 enzymes: SgfI *
March 22, 2012 17:38 ..
AclI AA'CG_TT
Cuts at: 0 570 1527 3159
Size: 570 957 1632
Fragments arranged by size:
1632 957 570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 2839 3026 3119 3159
Size: 1151 1688 187 93 40
Fragments arranged by size:
1688 1151 187 93 40
AflIII A'CryG_T
Cuts at: 0 2668 3159
Size: 2668 491
AleI CACnn'nnGTG
Cuts at: 0 2021 3159
Size: 2021 1138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2851 3159
Size: 2851 308
AlwNI CAG_nnn'CTG
Cuts at: 0 1120 1174 3004 3159
Size: 1120 54 1830 155
Fragments arranged by size:
1830 1120 155 54
ApoI r'AATT_y
Cuts at: 0 811 1948 2488 2596 2803 3159
Size: 811 1137 540 108 207 356
Fragments arranged by size:
1137 811 540 356 207 108
BanI G'GyrC_C
Cuts at: 0 70 161 3159
Size: 70 91 2998
Fragments arranged by size:
2998 91 70
BanII G_rGCy'C
Cuts at: 0 1157 2242 3159
Size: 1157 1085 917
Fragments arranged by size:
1157 1085 917
BbsI GAAGACnn'nnnn_
Cuts at: 0 1409 2089 2155 3159
Size: 1409 680 66 1004
Fragments arranged by size:
1409 1004 680 66
BbvCI CC'TCA_GC
Cuts at: 0 1385 3159
Size: 1385 1774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 77 156 190 3159
Size: 43 34 79 34 2969
Fragments arranged by size:
2969 79 43 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 534 3023 3159
Size: 534 2489 136
Fragments arranged by size:
2489 534 136
BfrBI ATG'CAT
Cuts at: 0 2038 3159
Size: 2038 1121
BglII A'GATC_T
Cuts at: 0 720 2337 3159
Size: 720 1617 822
Fragments arranged by size:
1617 822 720
BlpI GC'TnA_GC
Cuts at: 0 1121 3159
Size: 1121 2038
Bme1580I G_kGCm'C
Cuts at: 0 73 3159
Size: 73 3086
BmgBI CAC'GTC
Cuts at: 0 1898 3159
Size: 1898 1261
BmtI G_CTAG'C
Cuts at: 0 1037 3159
Size: 1037 2122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 455 3159
Size: 205 250 2704
Fragments arranged by size:
2704 250 205
Bpu10I CC'TnA_GC
Cuts at: 0 466 1151 1385 3159
Size: 466 685 234 1774
Fragments arranged by size:
1774 685 466 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2861 2924 2968 3159
Size: 2861 63 44 191
Fragments arranged by size:
2861 191 63 44
BsaAI yAC'GTr
Cuts at: 0 120 3159
Size: 120 3039
BsaBI GATnn'nnATC
Cuts at: 0 1214 3159
Size: 1214 1945
BsaHI Gr'CG_yC
Cuts at: 0 210 3159
Size: 210 2949
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 468 498 1008 1038 3159
Size: 468 30 510 30 2121
Fragments arranged by size:
2121 510 468 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 1842 3159
Size: 1842 1317
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1731 3159
Size: 1731 1428
Bsp1286I G_dGCh'C
Cuts at: 0 73 1157 1842 2242 3159
Size: 73 1084 685 400 917
Fragments arranged by size:
1084 917 685 400 73
BspHI T'CATG_A
Cuts at: 0 753 3159
Size: 753 2406
BsrDI GCAATG_nn'
Cuts at: 0 87 3159
Size: 87 3072
BsrGI T'GTAC_A
Cuts at: 0 918 3159
Size: 918 2241
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 2050 3159
Size: 561 1489 1109
Fragments arranged by size:
1489 1109 561
BstYI r'GATC_y
Cuts at: 0 720 768 2337 3159
Size: 720 48 1569 822
Fragments arranged by size:
1569 822 720 48
Bsu36I CC'TnA_GG
Cuts at: 0 2282 3159
Size: 2282 877
BtgI C'CryG_G
Cuts at: 0 2535 3159
Size: 2535 624
BtsI GCAGTG_nn'
Cuts at: 0 1044 2937 3159
Size: 1044 1893 222
Fragments arranged by size:
1893 1044 222
ClaI AT'CG_AT
Cuts at: 0 372 3159
Size: 372 2787
DraI TTT'AAA
Cuts at: 0 1442 3159
Size: 1442 1717
EarI CTCTTCn'nnn_
Cuts at: 0 662 929 1881 2544 3002 3159
Size: 662 267 952 663 458 157
Fragments arranged by size:
952 663 662 458 267 157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 455 1151 2839 3026 3119 3159
Size: 205 250 696 1688 187 93 40
Fragments arranged by size:
1688 696 250 205 187 93 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 3159
Size: 223 2936
EcoO109I rG'GnC_Cy
Cuts at: 0 216 241 3159
Size: 216 25 2918
Fragments arranged by size:
2918 216 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 810 1500 2869 3159
Size: 810 690 1369 290
Fragments arranged by size:
1369 810 690 290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 1162 1194 1858 1890 2085 2117
Size: 415 32 715 32 664 32 195 32
Cuts at: 2117 2680 2712 3001 3033 3159
Size: 563 32 289 32 126
Fragments arranged by size:
715 664 563 415 289 195 126 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 549 1431 3159
Size: 549 882 1728
Fragments arranged by size:
1728 882 549
HindIII A'AGCT_T
Cuts at: 0 2501 3159
Size: 2501 658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 511 653 2392 3159
Size: 386 125 142 1739 767
Fragments arranged by size:
1739 767 386 142 125
MslI CAynn'nnrTG
Cuts at: 0 1709 2021 2037 2366 2382 2658 2726 3159
Size: 1709 312 16 329 16 276 68 433
Fragments arranged by size:
1709 433 329 312 276 68 16 16
MspA1I CmG'CkG
Cuts at: 0 150 159 609 1384 3159
Size: 150 9 450 775 1775
Fragments arranged by size:
1775 775 450 150 9
NcoI C'CATG_G
Cuts at: 0 2535 3159
Size: 2535 624
NdeI CA'TA_TG
Cuts at: 0 1963 2006 2174 2795 3159
Size: 1963 43 168 621 364
Fragments arranged by size:
1963 621 364 168 43
NheI G'CTAG_C
Cuts at: 0 1033 3159
Size: 1033 2126
NsiI A_TGCA'T
Cuts at: 0 2040 3159
Size: 2040 1119
NspI r_CATG'y
Cuts at: 0 2038 2042 2521 2672 2890 2916 3159
Size: 2038 4 479 151 218 26 243
Fragments arranged by size:
2038 479 243 218 151 26 4
PciI A'CATG_T
Cuts at: 0 2668 3159
Size: 2668 491
PpuMI rG'GwC_Cy
Cuts at: 0 216 241 3159
Size: 216 25 2918
Fragments arranged by size:
2918 216 25
PsiI TTA'TAA
Cuts at: 0 1753 3159
Size: 1753 1406
PstI C_TGCA'G
Cuts at: 0 200 1586 3159
Size: 200 1386 1573
Fragments arranged by size:
1573 1386 200
PvuII CAG'CTG
Cuts at: 0 609 1384 3159
Size: 609 775 1775
Fragments arranged by size:
1775 775 609
SanDI GG'GwC_CC
Cuts at: 0 241 3159
Size: 241 2918
SapI GCTCTTCn'nnn_
Cuts at: 0 2544 3159
Size: 2544 615
SfcI C'TryA_G
Cuts at: 0 196 491 846 1582 2406 3159
Size: 196 295 355 736 824 753
Fragments arranged by size:
824 753 736 355 295 196
SmlI C'TyrA_G
Cuts at: 0 2876 2939 2947 3159
Size: 2876 63 8 212
Fragments arranged by size:
2876 212 63 8
SphI G_CATG'C
Cuts at: 0 2042 2916 3159
Size: 2042 874 243
Fragments arranged by size:
2042 874 243
StyI C'CwwG_G
Cuts at: 0 2535 2868 3159
Size: 2535 333 291
Fragments arranged by size:
2535 333 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1003 3159
Size: 1003 2156
TatI w'GTAC_w
Cuts at: 0 671 828 918 969 1002 1089 1820 2302
Size: 671 157 90 51 33 87 731 482
Cuts at: 2302 3159
Size: 857
Fragments arranged by size:
857 731 671 482 157 90 87 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 2560 2592 2864 2896 3159
Size: 271 32 2257 32 272 32 263
Fragments arranged by size:
2257 272 271 263 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1406 2116 2377 3113 3159
Size: 1406 710 261 736 46
Fragments arranged by size:
1406 736 710 261 46
Enzymes that do cut:
AclI AcuI AflIII AleI AlfI AlwNI ApoI BanI
BanII BbsI BbvCI BcgI BciVI BfrBI BglII BlpI
Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaXI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrGI
BstXI BstYI Bsu36I BtgI BtsI ClaI DraI EarI
Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI
MslI MspA1I NcoI NdeI NheI NsiI NspI PciI
PpuMI PsiI PstI PvuII SanDI SapI SfcI SmlI
SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BclI BglI BmrI BplI BsaI
BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BspEI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI