Restriction map for
pFN21AE4258
(Linear) (Six-base) MAPSORT of: KIEE4258.seq Check: 7191 from: 1 to: 2943
KIEE4258.orf
With 182 enzymes: SgfI *
July 3, 2012 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 199 2943
Size: 199 2744
AccI GT'mk_AC
Cuts at: 0 18 1189 2786 2943
Size: 18 1171 1597 157
Fragments arranged by size:
1597 1171 157 18
Acc65I G'GTAC_C
Cuts at: 0 2915 2943
Size: 2915 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 2378 2492 2943
Size: 530 1848 114 451
Fragments arranged by size:
1848 530 451 114
AhdI GACnn_n'nnGTC
Cuts at: 0 2793 2943
Size: 2793 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 2943
Size: 726 2217
AlwNI CAG_nnn'CTG
Cuts at: 0 1363 2860 2943
Size: 1363 1497 83
Fragments arranged by size:
1497 1363 83
ApoI r'AATT_y
Cuts at: 0 644 910 1179 2539 2624 2943
Size: 644 266 269 1360 85 319
Fragments arranged by size:
1360 644 319 269 266 85
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2899 2907 2932 2940 2943
Size: 2899 8 25 8 3
Fragments arranged by size:
2899 25 8 8 3
BanI G'GyrC_C
Cuts at: 0 2915 2943
Size: 2915 28
BanII G_rGCy'C
Cuts at: 0 82 1041 1325 2015 2943
Size: 82 959 284 690 928
Fragments arranged by size:
959 928 690 284 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 1505 2117 2943
Size: 327 1178 612 826
Fragments arranged by size:
1178 826 612 327
BfrBI ATG'CAT
Cuts at: 0 312 2943
Size: 312 2631
BglII A'GATC_T
Cuts at: 0 346 2943
Size: 346 2597
BmrI ACTGGGnnnn_n'
Cuts at: 0 2086 2452 2553 2915 2943
Size: 2086 366 101 362 28
Fragments arranged by size:
2086 366 362 101 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 893 1149 2209 2943
Size: 853 40 256 1060 734
Fragments arranged by size:
1060 853 734 256 40
Bpu10I CC'TnA_GC
Cuts at: 0 1757 2450 2943
Size: 1757 693 493
Fragments arranged by size:
1757 693 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1672 1714 1971 2943
Size: 1672 42 257 972
Fragments arranged by size:
1672 972 257 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 1782 2943
Size: 1782 1161
BsaAI yAC'GTr
Cuts at: 0 2152 2943
Size: 2152 791
BsaWI w'CCGG_w
Cuts at: 0 696 1463 2943
Size: 696 767 1480
Fragments arranged by size:
1480 767 696
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 2943
Size: 85 30 2828
Fragments arranged by size:
2828 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 297 2943
Size: 297 2646
BseYI C'CCAG_C
Cuts at: 0 653 2090 2943
Size: 653 1437 853
Fragments arranged by size:
1437 853 653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 2943
Size: 227 2716
BsiHKAI G_wGCw'C
Cuts at: 0 1041 1325 2015 2451 2943
Size: 1041 284 690 436 492
Fragments arranged by size:
1041 690 492 436 284
BsmI GAATG_Cn'
Cuts at: 0 2183 2943
Size: 2183 760
Bsp1286I G_dGCh'C
Cuts at: 0 82 1041 1325 2015 2451 2943
Size: 82 959 284 690 436 492
Fragments arranged by size:
959 690 492 436 284 82
BspEI T'CCGG_A
Cuts at: 0 1463 2943
Size: 1463 1480
BspHI T'CATG_A
Cuts at: 0 1344 2943
Size: 1344 1599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 199 568 628 2943
Size: 199 369 60 2315
Fragments arranged by size:
2315 369 199 60
BsrDI GCAATG_nn'
Cuts at: 0 1340 2943
Size: 1340 1603
BsrGI T'GTAC_A
Cuts at: 0 2759 2943
Size: 2759 184
BstBI TT'CG_AA
Cuts at: 0 300 2943
Size: 300 2643
BstEII G'GTnAC_C
Cuts at: 0 2231 2943
Size: 2231 712
BstYI r'GATC_y
Cuts at: 0 346 1466 2165 2714 2943
Size: 346 1120 699 549 229
Fragments arranged by size:
1120 699 549 346 229
BstZ17I GTA'TAC
Cuts at: 0 19 2943
Size: 19 2924
Bsu36I CC'TnA_GG
Cuts at: 0 1720 2868 2943
Size: 1720 1148 75
Fragments arranged by size:
1720 1148 75
BtsI GCAGTG_nn'
Cuts at: 0 2521 2669 2858 2943
Size: 2521 148 189 85
Fragments arranged by size:
2521 189 148 85
ClaI AT'CG_AT
Cuts at: 0 1551 2943
Size: 1551 1392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 71 106 2645 2680 2943
Size: 71 35 2539 35 263
Fragments arranged by size:
2539 263 71 35 35
DraI TTT'AAA
Cuts at: 0 981 2943
Size: 981 1962
EarI CTCTTCn'nnn_
Cuts at: 0 400 595 1998 2943
Size: 400 195 1403 945
Fragments arranged by size:
1403 945 400 195
EcoICRI GAG'CTC
Cuts at: 0 1039 1323 2013 2943
Size: 1039 284 690 930
Fragments arranged by size:
1039 930 690 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 853 893 1149 2209 2378 2492 2943
Size: 530 323 40 256 1060 169 114 451
Fragments arranged by size:
1060 530 451 323 256 169 114 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 368 1719 2943
Size: 368 1351 1224
Fragments arranged by size:
1351 1224 368
EcoO109I rG'GnC_Cy
Cuts at: 0 420 2943
Size: 420 2523
EcoRI G'AATT_C
Cuts at: 0 2539 2943
Size: 2539 404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 729 2943
Size: 729 2214
HaeII r_GCGC'y
Cuts at: 0 1524 2943
Size: 1524 1419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 615 647 866 898 976 1008
Size: 336 32 247 32 219 32 78 32
Cuts at: 1008 1903 1935 1973 2005 2257 2289 2779 2811
Size: 895 32 38 32 252 32 490 32
Cuts at: 2811 2943
Size: 132
Fragments arranged by size:
895 490 336 252 247 219 132 78
38 32 32 32 32 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 264 295 1154 1747 2943
Size: 264 31 859 593 1196
Fragments arranged by size:
1196 859 593 264 31
KpnI G_GTAC'C
Cuts at: 0 2919 2943
Size: 2919 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1014 2943
Size: 71 943 1929
Fragments arranged by size:
1929 943 71
MslI CAynn'nnrTG
Cuts at: 0 2176 2943
Size: 2176 767
MspA1I CmG'CkG
Cuts at: 0 397 831 1296 1539 2793 2943
Size: 397 434 465 243 1254 150
Fragments arranged by size:
1254 465 434 397 243 150
NsiI A_TGCA'T
Cuts at: 0 314 2943
Size: 314 2629
NspI r_CATG'y
Cuts at: 0 180 504 2943
Size: 180 324 2439
Fragments arranged by size:
2439 324 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 1687 2943
Size: 1687 1256
PfoI T'CCnGG_A
Cuts at: 0 2896 2943
Size: 2896 47
PpuMI rG'GwC_Cy
Cuts at: 0 420 2943
Size: 420 2523
PshAI GACnn'nnGTC
Cuts at: 0 2771 2943
Size: 2771 172
PstI C_TGCA'G
Cuts at: 0 2664 2943
Size: 2664 279
PvuII CAG'CTG
Cuts at: 0 397 831 1296 1539 2793 2943
Size: 397 434 465 243 1254 150
Fragments arranged by size:
1254 465 434 397 243 150
SacI G_AGCT'C
Cuts at: 0 1041 1325 2015 2943
Size: 1041 284 690 928
Fragments arranged by size:
1041 928 690 284
ScaI AGT'ACT
Cuts at: 0 2441 2943
Size: 2441 502
SfcI C'TryA_G
Cuts at: 0 560 1470 2239 2660 2783 2943
Size: 560 910 769 421 123 160
Fragments arranged by size:
910 769 560 421 160 123
SmlI C'TyrA_G
Cuts at: 0 1651 1729 1950 2943
Size: 1651 78 221 993
Fragments arranged by size:
1651 993 221 78
SnaBI TAC'GTA
Cuts at: 0 2152 2943
Size: 2152 791
SpeI A'CTAG_T
Cuts at: 0 1089 2943
Size: 1089 1854
SspI AAT'ATT
Cuts at: 0 1695 2943
Size: 1695 1248
StuI AGG'CCT
Cuts at: 0 2399 2943
Size: 2399 544
StyI C'CwwG_G
Cuts at: 0 141 2295 2943
Size: 141 2154 648
Fragments arranged by size:
2154 648 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 140 357 1773 2129 2434 2943
Size: 140 217 1416 356 305 509
Fragments arranged by size:
1416 509 356 305 217 140
TatI w'GTAC_w
Cuts at: 0 2439 2759 2851 2943
Size: 2439 320 92 92
Fragments arranged by size:
2439 320 92 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 678 710 1606 1638 1734 1766
Size: 122 32 524 32 896 32 96 32
Cuts at: 1766 2699 2731 2943
Size: 933 32 212
Fragments arranged by size:
933 896 524 212 122 96 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2062 2943
Size: 2062 881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 732 2342 2943
Size: 162 570 1610 601
Fragments arranged by size:
1610 601 570 162
XmnI GAAnn'nnTTC
Cuts at: 0 841 2943
Size: 841 2102
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AlfI AlwNI ApoI
BaeI BanI BanII BbsI BfrBI BglII BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I
NsiI NspI PflMI PfoI PpuMI PshAI PstI PvuII
SacI ScaI SfcI SmlI SnaBI SpeI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmgBI BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmBI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BtgI BtgZI
DraIII DrdI EaeI EagI EciI EcoRV FseI FspI
FspAI HincII HpaI KasI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI XbaI XhoI XmaI ZraI