Restriction map for
pFN21AE4256
(Linear) (Six-base) MAPSORT of: KIEE4256.seq Check: 2408 from: 1 to: 3075
KIEE4256.orf
With 182 enzymes: SgfI *
July 3, 2012 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1012 3075
Size: 1012 2063
AccI GT'mk_AC
Cuts at: 0 3056 3075
Size: 3056 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 891 1944 2295 3075
Size: 46 845 1053 351 780
Fragments arranged by size:
1053 845 780 351 46
AfeI AGC'GCT
Cuts at: 0 2172 3075
Size: 2172 903
AhdI GACnn_n'nnGTC
Cuts at: 0 2040 3075
Size: 2040 1035
AleI CACnn'nnGTG
Cuts at: 0 1006 3075
Size: 1006 2069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3010 3075
Size: 3010 65
AlwNI CAG_nnn'CTG
Cuts at: 0 435 1447 1467 2418 2712 3003 3021 3075
Size: 435 1012 20 951 294 291 18 54
Fragments arranged by size:
1012 951 435 294 291 54 20 18
ApaI G_GGCC'C
Cuts at: 0 2117 3075
Size: 2117 958
ApaLI G'TGCA_C
Cuts at: 0 456 493 3046 3075
Size: 456 37 2553 29
Fragments arranged by size:
2553 456 37 29
ApoI r'AATT_y
Cuts at: 0 1453 3075
Size: 1453 1622
AvaI C'yCGr_G
Cuts at: 0 226 274 314 1083 1218 1436 3068 3075
Size: 226 48 40 769 135 218 1632 7
Fragments arranged by size:
1632 769 226 218 135 48 40 7
BamHI G'GATC_C
Cuts at: 0 3006 3075
Size: 3006 69
BanI G'GyrC_C
Cuts at: 0 377 1087 1673 1771 1888 3075
Size: 377 710 586 98 117 1187
Fragments arranged by size:
1187 710 586 377 117 98
BanII G_rGCy'C
Cuts at: 0 830 974 1789 1933 2117 3075
Size: 830 144 815 144 184 958
Fragments arranged by size:
958 830 815 184 144 144
BbeI G_GCGC'C
Cuts at: 0 381 1892 3075
Size: 381 1511 1183
Fragments arranged by size:
1511 1183 381
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 2488 3075
Size: 34 2454 587
Fragments arranged by size:
2454 587 34
BbvCI CC'TCA_GC
Cuts at: 0 791 1903 2206 2839 3075
Size: 791 1112 303 633 236
Fragments arranged by size:
1112 791 633 303 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 940 974 2643 2677 3075
Size: 940 34 1669 34 398
Fragments arranged by size:
1669 940 398 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 424 538 758 1105 1522 1846 2121 2496
Size: 424 114 220 347 417 324 275 375
Cuts at: 2496 3075
Size: 579
Fragments arranged by size:
579 424 417 375 347 324 275 220
114
BlpI GC'TnA_GC
Cuts at: 0 88 1879 2125 2174 3075
Size: 88 1791 246 49 901
Fragments arranged by size:
1791 901 246 88 49
Bme1580I G_kGCm'C
Cuts at: 0 405 460 497 1084 1776 2117 2214 3050
Size: 405 55 37 587 692 341 97 836
Cuts at: 3050 3075
Size: 25
Fragments arranged by size:
836 692 587 405 341 97 55 37
25
BmgBI CAC'GTC
Cuts at: 0 309 2037 3075
Size: 309 1728 1038
Fragments arranged by size:
1728 1038 309
BmrI ACTGGGnnnn_n'
Cuts at: 0 348 2224 3075
Size: 348 1876 851
Fragments arranged by size:
1876 851 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 301 820 988 1003 1933 2068 2782
Size: 55 246 519 168 15 930 135 714
Cuts at: 2782 2968 3075
Size: 186 107
Fragments arranged by size:
930 714 519 246 186 168 135 107
55 15
Bpu10I CC'TnA_GC
Cuts at: 0 436 791 1903 2206 2839 3075
Size: 436 355 1112 303 633 236
Fragments arranged by size:
1112 633 436 355 303 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2368 2911 3075
Size: 2368 543 164
Fragments arranged by size:
2368 543 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 1044 3075
Size: 1044 2031
BsaAI yAC'GTr
Cuts at: 0 2187 3075
Size: 2187 888
BsaBI GATnn'nnATC
Cuts at: 0 808 3075
Size: 808 2267
BsaHI Gr'CG_yC
Cuts at: 0 378 821 836 1068 1145 1570 1825 1889
Size: 378 443 15 232 77 425 255 64
Cuts at: 1889 3075
Size: 1186
Fragments arranged by size:
1186 443 425 378 255 232 77 64
15
BsaWI w'CCGG_w
Cuts at: 0 1421 1533 2477 3075
Size: 1421 112 944 598
Fragments arranged by size:
1421 944 598 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 1024 1054 2146 2176 2299 2329
Size: 160 30 834 30 1092 30 123 30
Cuts at: 2329 2755 2785 3049 3075
Size: 426 30 264 26
Fragments arranged by size:
1092 834 426 264 160 123 30 30
30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 733 1891 1923 2065 2341 2344 2356
Size: 298 435 1158 32 142 276 3 12
Cuts at: 2356 3075
Size: 719
Fragments arranged by size:
1158 719 435 298 276 142 32 12
3
BseYI C'CCAG_C
Cuts at: 0 200 500 962 1594 1630 2461 3075
Size: 200 300 462 632 36 831 614
Fragments arranged by size:
831 632 614 462 300 200 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 493 2218 2311 3016 3075
Size: 430 63 1725 93 705 59
Fragments arranged by size:
1725 705 430 93 63 59
BsiEI CG_ry'CG
Cuts at: 0 342 2982 3075
Size: 342 2640 93
Fragments arranged by size:
2640 342 93
BsiHKAI G_wGCw'C
Cuts at: 0 323 460 497 830 974 1307 2180 2830
Size: 323 137 37 333 144 333 873 650
Cuts at: 2830 3050 3075
Size: 220 25
Fragments arranged by size:
873 650 333 333 323 220 144 137
37 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 299 1816 3075
Size: 299 1517 1259
Fragments arranged by size:
1517 1259 299
Bsp1286I G_dGCh'C
Cuts at: 0 323 405 460 497 830 974 1084 1307
Size: 323 82 55 37 333 144 110 223
Cuts at: 1307 1776 1789 1933 2117 2180 2214 2830 3050
Size: 469 13 144 184 63 34 616 220
Cuts at: 3050 3075
Size: 25
Fragments arranged by size:
616 469 333 323 223 220 184 144
144 110 82 63 55 37 34 25
13
BspEI T'CCGG_A
Cuts at: 0 1421 1533 2477 3075
Size: 1421 112 944 598
Fragments arranged by size:
1421 944 598 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1012 3029 3075
Size: 1012 2017 46
Fragments arranged by size:
2017 1012 46
BsrBI CCG'CTC
Cuts at: 0 83 3075
Size: 83 2992
BsrFI r'CCGG_y
Cuts at: 0 341 565 1697 2490 3075
Size: 341 224 1132 793 585
Fragments arranged by size:
1132 793 585 341 224
BsrGI T'GTAC_A
Cuts at: 0 551 3075
Size: 551 2524
BssHII G'CGCG_C
Cuts at: 0 521 595 3075
Size: 521 74 2480
Fragments arranged by size:
2480 521 74
BssSI C'ACGA_G
Cuts at: 0 75 670 1942 3075
Size: 75 595 1272 1133
Fragments arranged by size:
1272 1133 595 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1059 3021 3075
Size: 1059 1962 54
Fragments arranged by size:
1962 1059 54
BstEII G'GTnAC_C
Cuts at: 0 2349 3075
Size: 2349 726
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 1008 2101 2443 3075
Size: 738 270 1093 342 632
Fragments arranged by size:
1093 738 632 342 270
BstYI r'GATC_y
Cuts at: 0 722 2054 2474 2502 2741 2819 2879 3006
Size: 722 1332 420 28 239 78 60 127
Cuts at: 3006 3075
Size: 69
Fragments arranged by size:
1332 722 420 239 127 78 69 60
28
Bsu36I CC'TnA_GG
Cuts at: 0 1996 3075
Size: 1996 1079
BtgI C'CryG_G
Cuts at: 0 147 265 1193 1322 1550 1920 1977 2198
Size: 147 118 928 129 228 370 57 221
Cuts at: 2198 2735 3075
Size: 537 340
Fragments arranged by size:
928 537 370 340 228 221 147 129
118 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1621 3075
Size: 1621 1454
BtsI GCAGTG_nn'
Cuts at: 0 1799 2106 2436 2811 3010 3075
Size: 1799 307 330 375 199 65
Fragments arranged by size:
1799 375 330 307 199 65
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2495 2530 3075
Size: 2495 35 545
Fragments arranged by size:
2495 545 35
EaeI y'GGCC_r
Cuts at: 0 2 242 339 383 509 740 998 1286
Size: 2 240 97 44 126 231 258 288
Cuts at: 1286 1980 2060 2492 2979 3075
Size: 694 80 432 487 96
Fragments arranged by size:
694 487 432 288 258 240 231 126
97 96 80 44 2
EagI C'GGCC_G
Cuts at: 0 339 2979 3075
Size: 339 2640 96
Fragments arranged by size:
2640 339 96
EarI CTCTTCn'nnn_
Cuts at: 0 135 256 3075
Size: 135 121 2819
Fragments arranged by size:
2819 135 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 351 776 3075
Size: 351 425 2299
Fragments arranged by size:
2299 425 351
EcoICRI GAG'CTC
Cuts at: 0 828 972 3075
Size: 828 144 2103
Fragments arranged by size:
2103 828 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 55 301 820 891 988 1003 1933
Size: 46 9 246 519 71 97 15 930
Cuts at: 1933 1944 2068 2295 2782 2968 3075
Size: 11 124 227 487 186 107
Fragments arranged by size:
930 519 487 246 227 186 124 107
97 71 46 15 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 715 1422 1942 2062 2152 3075
Size: 715 707 520 120 90 923
Fragments arranged by size:
923 715 707 520 120 90
EcoO109I rG'GnC_Cy
Cuts at: 0 231 795 926 1464 2113 2145 2258 2940
Size: 231 564 131 538 649 32 113 682
Cuts at: 2940 3000 3018 3075
Size: 60 18 57
Fragments arranged by size:
682 649 564 538 231 131 113 60
57 32 18
FspI TGC'GCA
Cuts at: 0 664 3075
Size: 664 2411
FspAI rTGC'GCAy
Cuts at: 0 664 3075
Size: 664 2411
HaeII r_GCGC'y
Cuts at: 0 124 335 381 435 482 508 544 566
Size: 124 211 46 54 47 26 36 22
Cuts at: 566 1892 2174 3075
Size: 1326 282 901
Fragments arranged by size:
1326 901 282 211 124 54 47 46
36 26 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 1024 1056 2026 2058 2235 2267
Size: 238 32 754 32 970 32 177 32
Cuts at: 2267 2299 2331 2694 2726 2755 2787 3075
Size: 32 32 363 32 29 32 288
Fragments arranged by size:
970 754 363 288 238 177 32 32
32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 1545 3075
Size: 1545 1530
HpaI GTT'AAC
Cuts at: 0 1545 3075
Size: 1545 1530
KasI G'GCGC_C
Cuts at: 0 377 1888 3075
Size: 377 1511 1187
Fragments arranged by size:
1511 1187 377
MfeI C'AATT_G
Cuts at: 0 1753 3075
Size: 1753 1322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 3075
Size: 134 2941
MscI TGG'CCA
Cuts at: 0 742 1000 1288 3075
Size: 742 258 288 1787
Fragments arranged by size:
1787 742 288 258
MslI CAynn'nnrTG
Cuts at: 0 1006 1744 2197 2441 3075
Size: 1006 738 453 244 634
Fragments arranged by size:
1006 738 634 453 244
MspA1I CmG'CkG
Cuts at: 0 149 200 267 500 635 966 1331 1836
Size: 149 51 67 233 135 331 365 505
Cuts at: 1836 1972 2776 2793 3075
Size: 136 804 17 282
Fragments arranged by size:
804 505 365 331 282 233 149 136
135 67 51 17
NaeI GCC'GGC
Cuts at: 0 343 567 1699 2492 3075
Size: 343 224 1132 793 583
Fragments arranged by size:
1132 793 583 343 224
NarI GG'CG_CC
Cuts at: 0 378 1889 3075
Size: 378 1511 1186
Fragments arranged by size:
1511 1186 378
NcoI C'CATG_G
Cuts at: 0 1193 1550 2198 2735 3075
Size: 1193 357 648 537 340
Fragments arranged by size:
1193 648 537 357 340
NgoMIV G'CCGG_C
Cuts at: 0 341 565 1697 2490 3075
Size: 341 224 1132 793 585
Fragments arranged by size:
1132 793 585 341 224
NspI r_CATG'y
Cuts at: 0 1062 1482 1911 3075
Size: 1062 420 429 1164
Fragments arranged by size:
1164 1062 429 420
PasI CC'CwG_GG
Cuts at: 0 930 3075
Size: 930 2145
PflMI CCAn_nnn'nTGG
Cuts at: 0 1133 1467 1926 2418 3075
Size: 1133 334 459 492 657
Fragments arranged by size:
1133 657 492 459 334
PfoI T'CCnGG_A
Cuts at: 0 2759 3075
Size: 2759 316
PpuMI rG'GwC_Cy
Cuts at: 0 231 926 1464 2258 3018 3075
Size: 231 695 538 794 760 57
Fragments arranged by size:
794 760 695 538 231 57
PspOMI G'GGCC_C
Cuts at: 0 2113 3075
Size: 2113 962
PstI C_TGCA'G
Cuts at: 0 1835 2807 3017 3026 3075
Size: 1835 972 210 9 49
Fragments arranged by size:
1835 972 210 49 9
PvuII CAG'CTG
Cuts at: 0 200 500 966 1836 1972 2776 2793 3075
Size: 200 300 466 870 136 804 17 282
Fragments arranged by size:
870 804 466 300 282 200 136 17
SacI G_AGCT'C
Cuts at: 0 830 974 3075
Size: 830 144 2101
Fragments arranged by size:
2101 830 144
SacII CC_GC'GG
Cuts at: 0 150 268 3075
Size: 150 118 2807
Fragments arranged by size:
2807 150 118
SanDI GG'GwC_CC
Cuts at: 0 926 3075
Size: 926 2149
SexAI A'CCwGG_T
Cuts at: 0 710 3075
Size: 710 2365
SfcI C'TryA_G
Cuts at: 0 1831 2149 2166 2803 3013 3022 3075
Size: 1831 318 17 637 210 9 53
Fragments arranged by size:
1831 637 318 210 53 17 9
SfoI GGC'GCC
Cuts at: 0 379 1890 3075
Size: 379 1511 1185
Fragments arranged by size:
1511 1185 379
SgrAI Cr'CCGG_yG
Cuts at: 0 565 3075
Size: 565 2510
SmaI CCC'GGG
Cuts at: 0 316 1085 3070 3075
Size: 316 769 1985 5
Fragments arranged by size:
1985 769 316 5
SmlI C'TyrA_G
Cuts at: 0 2383 2926 3075
Size: 2383 543 149
Fragments arranged by size:
2383 543 149
SphI G_CATG'C
Cuts at: 0 1482 1911 3075
Size: 1482 429 1164
Fragments arranged by size:
1482 1164 429
StuI AGG'CCT
Cuts at: 0 1525 3075
Size: 1525 1550
StyI C'CwwG_G
Cuts at: 0 47 1193 1526 1550 2141 2198 2735 3075
Size: 47 1146 333 24 591 57 537 340
Fragments arranged by size:
1146 591 537 340 333 57 47 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 2368 2449 3075
Size: 313 2055 81 626
Fragments arranged by size:
2055 626 313 81
TatI w'GTAC_w
Cuts at: 0 551 2162 2333 2714 3075
Size: 551 1611 171 381 361
Fragments arranged by size:
1611 551 381 361 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2204 2236 2564 2596 3075
Size: 2204 32 328 32 479
Fragments arranged by size:
2204 479 328 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1036 2404 3075
Size: 1036 1368 671
Fragments arranged by size:
1368 1036 671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 754 1524 2757 3075
Size: 754 770 1233 318
Fragments arranged by size:
1233 770 754 318
XmaI C'CCGG_G
Cuts at: 0 314 1083 3068 3075
Size: 314 769 1985 7
Fragments arranged by size:
1985 769 314 7
XmnI GAAnn'nnTTC
Cuts at: 0 1957 3075
Size: 1957 1118
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HincII HpaI KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SexAI SfcI SfoI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AloI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII
BmtI BplI BsiWI BsmI BspHI BsrDI BstBI BstZ17I
ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI
HindIII KpnI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI
SgfI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
ZraI