Restriction map for
pFN21AE4254
(Linear) (Six-base) MAPSORT of: KIEE4254.seq Check: 4070 from: 1 to: 3027
KIEE4254.orf
With 182 enzymes: SgfI *
March 22, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 0 1558 1721 2201 3027
Size: 1558 163 480 826
Fragments arranged by size:
1558 826 480 163
AccI GT'mk_AC
Cuts at: 0 1289 3027
Size: 1289 1738
AclI AA'CG_TT
Cuts at: 0 2767 3027
Size: 2767 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 898 1707 3027
Size: 254 644 809 1320
Fragments arranged by size:
1320 809 644 254
AflII C'TTAA_G
Cuts at: 0 297 3027
Size: 297 2730
AflIII A'CryG_T
Cuts at: 0 548 674 1506 3027
Size: 548 126 832 1521
Fragments arranged by size:
1521 832 548 126
AleI CACnn'nnGTG
Cuts at: 0 217 3027
Size: 217 2810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2909 3027
Size: 2909 118
AlwNI CAG_nnn'CTG
Cuts at: 0 91 1062 1669 1758 1839 2730 2817 3027
Size: 91 971 607 89 81 891 87 210
Fragments arranged by size:
971 891 607 210 91 89 87 81
ApaI G_GGCC'C
Cuts at: 0 2571 3027
Size: 2571 456
AvaI C'yCGr_G
Cuts at: 0 1990 2314 2958 3027
Size: 1990 324 644 69
Fragments arranged by size:
1990 644 324 69
BamHI G'GATC_C
Cuts at: 0 2759 3027
Size: 2759 268
BanI G'GyrC_C
Cuts at: 0 2064 2182 2704 3027
Size: 2064 118 522 323
Fragments arranged by size:
2064 522 323 118
BanII G_rGCy'C
Cuts at: 0 7 1570 2255 2571 2719 2959 3027
Size: 7 1563 685 316 148 240 68
Fragments arranged by size:
1563 685 316 240 148 68 7
BbeI G_GCGC'C
Cuts at: 0 2186 3027
Size: 2186 841
BbsI GAAGACnn'nnnn_
Cuts at: 0 1288 1460 1713 1721 2122 3027
Size: 1288 172 253 8 401 905
Fragments arranged by size:
1288 905 401 253 172 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1570 1604 2203 2237 3027
Size: 1570 34 599 34 790
Fragments arranged by size:
1570 790 599 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 581 3027
Size: 581 2446
BglI GCCn_nnn'nGGC
Cuts at: 0 200 2016 2856 3027
Size: 200 1816 840 171
Fragments arranged by size:
1816 840 200 171
BglII A'GATC_T
Cuts at: 0 1116 3027
Size: 1116 1911
BlpI GC'TnA_GC
Cuts at: 0 2302 2365 3027
Size: 2302 63 662
Fragments arranged by size:
2302 662 63
Bme1580I G_kGCm'C
Cuts at: 0 1794 2069 2571 2637 2707 3027
Size: 1794 275 502 66 70 320
Fragments arranged by size:
1794 502 320 275 70 66
BmgBI CAC'GTC
Cuts at: 0 864 2952 3027
Size: 864 2088 75
Fragments arranged by size:
2088 864 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 920 1768 2042 3027
Size: 920 848 274 985
Fragments arranged by size:
985 920 848 274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 1995 3027
Size: 952 1043 1032
Fragments arranged by size:
1043 1032 952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1935 2155 2278 2558 2852 2893 3027
Size: 829 1106 220 123 280 294 41 134
Fragments arranged by size:
1106 829 294 280 220 134 123 41
Bpu10I CC'TnA_GC
Cuts at: 0 1571 3001 3027
Size: 1571 1430 26
Fragments arranged by size:
1571 1430 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 2769 2849 3027
Size: 922 1847 80 178
Fragments arranged by size:
1847 922 178 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 22 903 1512 2547 2651 2797 2828 2906
Size: 22 881 609 1035 104 146 31 78
Cuts at: 2906 3010 3027
Size: 104 17
Fragments arranged by size:
1035 881 609 146 104 104 78 31
22 17
BsaAI yAC'GTr
Cuts at: 0 675 3027
Size: 675 2352
BsaBI GATnn'nnATC
Cuts at: 0 592 3027
Size: 592 2435
BsaHI Gr'CG_yC
Cuts at: 0 1555 1718 2183 2198 2339 3027
Size: 1555 163 465 15 141 688
Fragments arranged by size:
1555 688 465 163 141 15
BsaWI w'CCGG_w
Cuts at: 0 1042 1223 3027
Size: 1042 181 1804
Fragments arranged by size:
1804 1042 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 422 452 1024 1054 3027
Size: 422 30 572 30 1973
Fragments arranged by size:
1973 572 422 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1009 1875 2074 2888 3027
Size: 1009 866 199 814 139
Fragments arranged by size:
1009 866 814 199 139
BseYI C'CCAG_C
Cuts at: 0 490 1674 1945 1956 2127 2730 2739 3027
Size: 490 1184 271 11 171 603 9 288
Fragments arranged by size:
1184 603 490 288 271 171 11 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 388 1394 3027
Size: 118 270 1006 1633
Fragments arranged by size:
1633 1006 270 118
BsiHKAI G_wGCw'C
Cuts at: 0 224 723 3027
Size: 224 499 2304
Fragments arranged by size:
2304 499 224
BsmI GAATG_Cn'
Cuts at: 0 453 3027
Size: 453 2574
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2205 3027
Size: 2205 822
Bsp1286I G_dGCh'C
Cuts at: 0 7 224 723 1570 1794 2069 2255 2571
Size: 7 217 499 847 224 275 186 316
Cuts at: 2571 2637 2707 2719 2959 3027
Size: 66 70 12 240 68
Fragments arranged by size:
847 499 316 275 240 224 217 186
70 68 66 12 7
BspEI T'CCGG_A
Cuts at: 0 1042 1223 3027
Size: 1042 181 1804
Fragments arranged by size:
1804 1042 181
BsrBI CCG'CTC
Cuts at: 0 1040 2208 2301 2364 2783 3027
Size: 1040 1168 93 63 419 244
Fragments arranged by size:
1168 1040 419 244 93 63
BsrDI GCAATG_nn'
Cuts at: 0 1358 3027
Size: 1358 1669
BsrFI r'CCGG_y
Cuts at: 0 1457 2179 2589 3027
Size: 1457 722 410 438
Fragments arranged by size:
1457 722 438 410
BsrGI T'GTAC_A
Cuts at: 0 677 3027
Size: 677 2350
BssSI C'ACGA_G
Cuts at: 0 2328 2712 3027
Size: 2328 384 315
Fragments arranged by size:
2328 384 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1062 1650 3027
Size: 1062 588 1377
Fragments arranged by size:
1377 1062 588
BstYI r'GATC_y
Cuts at: 0 87 1116 1842 2759 3027
Size: 87 1029 726 917 268
Fragments arranged by size:
1029 917 726 268 87
Bsu36I CC'TnA_GG
Cuts at: 0 92 1804 2824 3027
Size: 92 1712 1020 203
Fragments arranged by size:
1712 1020 203 92
BtgI C'CryG_G
Cuts at: 0 633 1866 2699 3027
Size: 633 1233 833 328
Fragments arranged by size:
1233 833 633 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 661 1518 3027
Size: 661 857 1509
Fragments arranged by size:
1509 857 661
BtsI GCAGTG_nn'
Cuts at: 0 1194 1544 1747 2340 2881 3016 3027
Size: 1194 350 203 593 541 135 11
Fragments arranged by size:
1194 593 541 350 203 135 11
DraIII CAC_nnn'GTG
Cuts at: 0 2194 3027
Size: 2194 833
DrdI GACnn_nn'nnGTC
Cuts at: 0 1413 3027
Size: 1413 1614
EaeI y'GGCC_r
Cuts at: 0 201 923 1274 1462 1937 2162 2688 3027
Size: 201 722 351 188 475 225 526 339
Fragments arranged by size:
722 526 475 351 339 225 201 188
EarI CTCTTCn'nnn_
Cuts at: 0 274 876 1093 1151 1385 3027
Size: 274 602 217 58 234 1642
Fragments arranged by size:
1642 602 274 234 217 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2652 3027
Size: 2652 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 829 898 1707 1935 2155 2278 2558
Size: 254 575 69 809 228 220 123 280
Cuts at: 2558 2852 2893 3027
Size: 294 41 134
Fragments arranged by size:
809 575 294 280 254 228 220 134
123 69 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 63 425 1924 3027
Size: 63 362 1499 1103
Fragments arranged by size:
1499 1103 362 63
EcoO109I rG'GnC_Cy
Cuts at: 0 1437 1666 1782 1820 2018 2278 2567 2568
Size: 1437 229 116 38 198 260 289 1
Cuts at: 2568 2734 2858 3027
Size: 166 124 169
Fragments arranged by size:
1437 289 260 229 198 169 166 124
116 38 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 303 3027
Size: 303 2724
HaeII r_GCGC'y
Cuts at: 0 2186 3027
Size: 2186 841
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 422 454 853 885 1827 1859 2946 2978
Size: 422 32 399 32 942 32 1087 32
Cuts at: 2978 3027
Size: 49
Fragments arranged by size:
1087 942 422 399 49 32 32 32
32
HincII GTy'rAC
Cuts at: 0 543 3027
Size: 543 2484
HindIII A'AGCT_T
Cuts at: 0 1888 3027
Size: 1888 1139
HpaI GTT'AAC
Cuts at: 0 543 3027
Size: 543 2484
KasI G'GCGC_C
Cuts at: 0 2182 3027
Size: 2182 845
MscI TGG'CCA
Cuts at: 0 203 925 1939 2690 3027
Size: 203 722 1014 751 337
Fragments arranged by size:
1014 751 722 337 203
MslI CAynn'nnrTG
Cuts at: 0 217 685 1537 3027
Size: 217 468 852 1490
Fragments arranged by size:
1490 852 468 217
MspA1I CmG'CkG
Cuts at: 0 1080 1398 1868 1960 2730 3027
Size: 1080 318 470 92 770 297
Fragments arranged by size:
1080 770 470 318 297 92
NaeI GCC'GGC
Cuts at: 0 2181 2591 3027
Size: 2181 410 436
Fragments arranged by size:
2181 436 410
NarI GG'CG_CC
Cuts at: 0 2183 3027
Size: 2183 844
NcoI C'CATG_G
Cuts at: 0 2699 3027
Size: 2699 328
NgoMIV G'CCGG_C
Cuts at: 0 2179 2589 3027
Size: 2179 410 438
Fragments arranged by size:
2179 438 410
NspI r_CATG'y
Cuts at: 0 552 1510 3027
Size: 552 958 1517
Fragments arranged by size:
1517 958 552
PasI CC'CwG_GG
Cuts at: 0 2583 3027
Size: 2583 444
PciI A'CATG_T
Cuts at: 0 548 1506 3027
Size: 548 958 1521
Fragments arranged by size:
1521 958 548
PflMI CCAn_nnn'nTGG
Cuts at: 0 665 3027
Size: 665 2362
PfoI T'CCnGG_A
Cuts at: 0 7 110 2354 3027
Size: 7 103 2244 673
Fragments arranged by size:
2244 673 103 7
PmlI CAC'GTG
Cuts at: 0 675 3027
Size: 675 2352
PpuMI rG'GwC_Cy
Cuts at: 0 1437 1782 1820 2734 3027
Size: 1437 345 38 914 293
Fragments arranged by size:
1437 914 345 293 38
PsiI TTA'TAA
Cuts at: 0 329 3027
Size: 329 2698
PspOMI G'GGCC_C
Cuts at: 0 2567 3027
Size: 2567 460
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 572 604 3027
Size: 572 32 2423
Fragments arranged by size:
2423 572 32
PstI C_TGCA'G
Cuts at: 0 1067 1085 1754 2216 2325 2460 2888 3027
Size: 1067 18 669 462 109 135 428 139
Fragments arranged by size:
1067 669 462 428 139 135 109 18
PvuII CAG'CTG
Cuts at: 0 1080 1398 1960 3027
Size: 1080 318 562 1067
Fragments arranged by size:
1080 1067 562 318
SacII CC_GC'GG
Cuts at: 0 1869 3027
Size: 1869 1158
SanDI GG'GwC_CC
Cuts at: 0 2734 3027
Size: 2734 293
SapI GCTCTTCn'nnn_
Cuts at: 0 274 1093 1385 3027
Size: 274 819 292 1642
Fragments arranged by size:
1642 819 292 274
SbfI CC_TGCA'GG
Cuts at: 0 2460 3027
Size: 2460 567
SfcI C'TryA_G
Cuts at: 0 120 139 1063 1081 1750 2212 2321 2456
Size: 120 19 924 18 669 462 109 135
Cuts at: 2456 2722 2884 3027
Size: 266 162 143
Fragments arranged by size:
924 669 462 266 162 143 135 120
109 19 18
SfoI GGC'GCC
Cuts at: 0 2184 3027
Size: 2184 843
SmaI CCC'GGG
Cuts at: 0 2316 3027
Size: 2316 711
SmlI C'TyrA_G
Cuts at: 0 297 937 2784 2864 3027
Size: 297 640 1847 80 163
Fragments arranged by size:
1847 640 297 163 80
SspI AAT'ATT
Cuts at: 0 523 3027
Size: 523 2504
StuI AGG'CCT
Cuts at: 0 2829 2870 3027
Size: 2829 41 157
Fragments arranged by size:
2829 157 41
StyI C'CwwG_G
Cuts at: 0 1433 2106 2274 2699 3027
Size: 1433 673 168 425 328
Fragments arranged by size:
1433 673 425 328 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2481 2541 2722 3027
Size: 2481 60 181 305
Fragments arranged by size:
2481 305 181 60
TatI w'GTAC_w
Cuts at: 0 662 677 3027
Size: 662 15 2350
Fragments arranged by size:
2350 662 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 1024 1056 1218 1250 3027
Size: 364 32 628 32 162 32 1777
Fragments arranged by size:
1777 628 364 162 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2076 2535 2814 3027
Size: 2076 459 279 213
Fragments arranged by size:
2076 459 279 213
XmaI C'CCGG_G
Cuts at: 0 2314 3027
Size: 2314 713
ZraI GAC'GTC
Cuts at: 0 1556 1719 2199 3027
Size: 1556 163 480 828
Fragments arranged by size:
1556 828 480 163
Enzymes that do cut:
AatII AccI AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII HpaI KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PsiI PspOMI PsrI PstI PvuII SacII
SanDI SapI SbfI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AgeI AhdI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI
BmtI BsiEI BsiWI BspHI BspMI BssHII BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PspXI PvuI RsrII SacI SalI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI XmnI