Restriction map for
pFN21AE4248
(Linear) (Six-base) MAPSORT of: KIEE4248.seq Check: 6391 from: 1 to: 2832
KIEE4248.orf
With 182 enzymes: SgfI *
March 22, 2012 17:38 ..
AccI GT'mk_AC
Cuts at: 0 2762 2832
Size: 2762 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 902 1249 1916 1999 2832
Size: 21 881 347 667 83 833
Fragments arranged by size:
881 833 667 347 83 21
AflIII A'CryG_T
Cuts at: 0 1787 2832
Size: 1787 1045
AgeI A'CCGG_T
Cuts at: 0 69 2832
Size: 69 2763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1819 2832
Size: 1819 1013
AlwNI CAG_nnn'CTG
Cuts at: 0 1431 2061 2685 2832
Size: 1431 630 624 147
Fragments arranged by size:
1431 630 624 147
ApaI G_GGCC'C
Cuts at: 0 573 951 1344 1380 2832
Size: 573 378 393 36 1452
Fragments arranged by size:
1452 573 393 378 36
ApaLI G'TGCA_C
Cuts at: 0 982 2832
Size: 982 1850
ApoI r'AATT_y
Cuts at: 0 87 562 583 774 2394 2832
Size: 87 475 21 191 1620 438
Fragments arranged by size:
1620 475 438 191 87 21
AseI AT'TA_AT
Cuts at: 0 962 1094 2832
Size: 962 132 1738
Fragments arranged by size:
1738 962 132
AvaI C'yCGr_G
Cuts at: 0 362 1359 1712 2832
Size: 362 997 353 1120
Fragments arranged by size:
1120 997 362 353
BanI G'GyrC_C
Cuts at: 0 166 1566 2832
Size: 166 1400 1266
Fragments arranged by size:
1400 1266 166
BanII G_rGCy'C
Cuts at: 0 573 787 951 1344 1380 1861 2318 2832
Size: 573 214 164 393 36 481 457 514
Fragments arranged by size:
573 514 481 457 393 214 164 36
BbeI G_GCGC'C
Cuts at: 0 170 1570 2832
Size: 170 1400 1262
Fragments arranged by size:
1400 1262 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 358 1904 2061 2822 2832
Size: 358 1546 157 761 10
Fragments arranged by size:
1546 761 358 157 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1587 1621 1713 1747 2832
Size: 1587 34 92 34 1085
Fragments arranged by size:
1587 1085 92 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1292 1418 2832
Size: 1292 126 1414
Fragments arranged by size:
1414 1292 126
BglII A'GATC_T
Cuts at: 0 2606 2832
Size: 2606 226
BlpI GC'TnA_GC
Cuts at: 0 397 743 1432 2425 2832
Size: 397 346 689 993 407
Fragments arranged by size:
993 689 407 397 346
Bme1580I G_kGCm'C
Cuts at: 0 363 573 951 986 1321 1344 1380 1964
Size: 363 210 378 35 335 23 36 584
Cuts at: 1964 2832
Size: 868
Fragments arranged by size:
868 584 378 363 335 210 36 35
23
BmgBI CAC'GTC
Cuts at: 0 348 2832
Size: 348 2484
BmrI ACTGGGnnnn_n'
Cuts at: 0 2262 2832
Size: 2262 570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1508 2226 2832
Size: 1508 718 606
Fragments arranged by size:
1508 718 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 801 946 1330 1492 1549 2832
Size: 102 699 145 384 162 57 1283
Fragments arranged by size:
1283 699 384 162 145 102 57
Bpu10I CC'TnA_GC
Cuts at: 0 627 2832
Size: 627 2205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 562 1296 1939 2002 2832
Size: 2 560 734 643 63 830
Fragments arranged by size:
830 734 643 560 63 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 1552 2568 2832
Size: 1552 1016 264
Fragments arranged by size:
1552 1016 264
BsaAI yAC'GTr
Cuts at: 0 1755 1788 1965 2832
Size: 1755 33 177 867
Fragments arranged by size:
1755 867 177 33
BsaHI Gr'CG_yC
Cuts at: 0 167 913 1567 2832
Size: 167 746 654 1265
Fragments arranged by size:
1265 746 654 167
BsaWI w'CCGG_w
Cuts at: 0 69 1481 2480 2832
Size: 69 1412 999 352
Fragments arranged by size:
1412 999 352 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 1014 1044 2832
Size: 370 30 614 30 1788
Fragments arranged by size:
1788 614 370 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 472 475 1449 2656 2832
Size: 406 66 3 974 1207 176
Fragments arranged by size:
1207 974 406 176 66 3
BseYI C'CCAG_C
Cuts at: 0 138 485 789 1314 1387 1631 2061 2832
Size: 138 347 304 525 73 244 430 771
Fragments arranged by size:
771 525 430 347 304 244 138 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 951 1765 2710 2832
Size: 144 807 814 945 122
Fragments arranged by size:
945 814 807 144 122
BsiEI CG_ry'CG
Cuts at: 0 1018 1287 1291 1482 2832
Size: 1018 269 4 191 1350
Fragments arranged by size:
1350 1018 269 191 4
BsiHKAI G_wGCw'C
Cuts at: 0 787 986 1024 1861 2318 2379 2832
Size: 787 199 38 837 457 61 453
Fragments arranged by size:
837 787 457 453 199 61 38
BsmI GAATG_Cn'
Cuts at: 0 894 2832
Size: 894 1938
Bsp1286I G_dGCh'C
Cuts at: 0 363 573 787 951 986 1024 1321 1344
Size: 363 210 214 164 35 38 297 23
Cuts at: 1344 1380 1861 1964 2318 2379 2832
Size: 36 481 103 354 61 453
Fragments arranged by size:
481 453 363 354 297 214 210 164
103 61 38 36 35 23
BspHI T'CATG_A
Cuts at: 0 1667 1826 2120 2832
Size: 1667 159 294 712
Fragments arranged by size:
1667 712 294 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2036 2832
Size: 2036 796
BsrBI CCG'CTC
Cuts at: 0 1053 1280 1649 2832
Size: 1053 227 369 1183
Fragments arranged by size:
1183 1053 369 227
BsrDI GCAATG_nn'
Cuts at: 0 659 2832
Size: 659 2173
BsrFI r'CCGG_y
Cuts at: 0 69 410 1286 1421 2832
Size: 69 341 876 135 1411
Fragments arranged by size:
1411 876 341 135 69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2037 2832
Size: 2037 795
BstYI r'GATC_y
Cuts at: 0 497 2606 2832
Size: 497 2109 226
Fragments arranged by size:
2109 497 226
Bsu36I CC'TnA_GG
Cuts at: 0 1349 2449 2639 2832
Size: 1349 1100 190 193
Fragments arranged by size:
1349 1100 193 190
BtgI C'CryG_G
Cuts at: 0 161 171 1413 1562 2832
Size: 161 10 1242 149 1270
Fragments arranged by size:
1270 1242 161 149 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2312 2832
Size: 2312 520
BtsI GCAGTG_nn'
Cuts at: 0 132 2461 2832
Size: 132 2329 371
Fragments arranged by size:
2329 371 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 306 341 2832
Size: 306 35 2491
Fragments arranged by size:
2491 306 35
EaeI y'GGCC_r
Cuts at: 0 1015 1121 1284 1288 1994 2832
Size: 1015 106 163 4 706 838
Fragments arranged by size:
1015 838 706 163 106 4
EagI C'GGCC_G
Cuts at: 0 1015 1284 1288 2832
Size: 1015 269 4 1544
Fragments arranged by size:
1544 1015 269 4
EarI CTCTTCn'nnn_
Cuts at: 0 335 2665 2832
Size: 335 2330 167
Fragments arranged by size:
2330 335 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 538 1525 2734 2832
Size: 538 987 1209 98
Fragments arranged by size:
1209 987 538 98
EcoICRI GAG'CTC
Cuts at: 0 785 1859 2316 2832
Size: 785 1074 457 516
Fragments arranged by size:
1074 785 516 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 102 801 902 946 1249 1330 1492
Size: 21 81 699 101 44 303 81 162
Cuts at: 1492 1549 1916 1999 2832
Size: 57 367 83 833
Fragments arranged by size:
833 699 367 303 162 101 83 81
81 57 44 21
EcoO109I rG'GnC_Cy
Cuts at: 0 474 569 570 947 948 993 1076 1340
Size: 474 95 1 377 1 45 83 264
Cuts at: 1340 1341 1376 1377 2634 2832
Size: 1 35 1 1257 198
Fragments arranged by size:
1257 474 377 264 198 95 83 45
35 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1959 2832
Size: 1959 873
FseI GG_CCGG'CC
Cuts at: 0 1290 2832
Size: 1290 1542
HaeII r_GCGC'y
Cuts at: 0 170 1570 2231 2832
Size: 170 1400 661 601
Fragments arranged by size:
1400 661 601 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 2567 2599 2832
Size: 370 32 2165 32 233
Fragments arranged by size:
2165 370 233 32 32
HincII GTy'rAC
Cuts at: 0 267 1161 2541 2667 2832
Size: 267 894 1380 126 165
Fragments arranged by size:
1380 894 267 165 126
HindIII A'AGCT_T
Cuts at: 0 60 2832
Size: 60 2772
KasI G'GCGC_C
Cuts at: 0 166 1566 2832
Size: 166 1400 1266
Fragments arranged by size:
1400 1266 166
MfeI C'AATT_G
Cuts at: 0 215 2832
Size: 215 2617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 413 716 866 1487 2832
Size: 90 323 303 150 621 1345
Fragments arranged by size:
1345 621 323 303 150 90
MscI TGG'CCA
Cuts at: 0 1123 2832
Size: 1123 1709
MslI CAynn'nnrTG
Cuts at: 0 320 1801 2125 2197 2832
Size: 320 1481 324 72 635
Fragments arranged by size:
1481 635 324 320 72
MspA1I CmG'CkG
Cuts at: 0 173 292 444 793 1324 1428 1635 2685
Size: 173 119 152 349 531 104 207 1050
Cuts at: 2685 2832
Size: 147
Fragments arranged by size:
1050 531 349 207 173 152 147 119
104
NaeI GCC'GGC
Cuts at: 0 1288 1423 2832
Size: 1288 135 1409
Fragments arranged by size:
1409 1288 135
NarI GG'CG_CC
Cuts at: 0 167 1567 2832
Size: 167 1400 1265
Fragments arranged by size:
1400 1265 167
NcoI C'CATG_G
Cuts at: 0 161 1413 1562 2832
Size: 161 1252 149 1270
Fragments arranged by size:
1270 1252 161 149
NdeI CA'TA_TG
Cuts at: 0 433 2370 2832
Size: 433 1937 462
Fragments arranged by size:
1937 462 433
NgoMIV G'CCGG_C
Cuts at: 0 1286 1421 2832
Size: 1286 135 1411
Fragments arranged by size:
1411 1286 135
NspI r_CATG'y
Cuts at: 0 1149 1491 1806 2280 2832
Size: 1149 342 315 474 552
Fragments arranged by size:
1149 552 474 342 315
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 2832
Size: 306 2526
PmlI CAC'GTG
Cuts at: 0 1788 1965 2832
Size: 1788 177 867
Fragments arranged by size:
1788 867 177
PpuMI rG'GwC_Cy
Cuts at: 0 474 993 2634 2832
Size: 474 519 1641 198
Fragments arranged by size:
1641 519 474 198
PshAI GACnn'nnGTC
Cuts at: 0 262 1834 2257 2832
Size: 262 1572 423 575
Fragments arranged by size:
1572 575 423 262
PsiI TTA'TAA
Cuts at: 0 883 2832
Size: 883 1949
PspOMI G'GGCC_C
Cuts at: 0 569 947 1340 1376 2832
Size: 569 378 393 36 1456
Fragments arranged by size:
1456 569 393 378 36
PstI C_TGCA'G
Cuts at: 0 443 482 1640 1769 1811 2033 2832
Size: 443 39 1158 129 42 222 799
Fragments arranged by size:
1158 799 443 222 129 42 39
PvuII CAG'CTG
Cuts at: 0 444 793 1324 1635 2685 2832
Size: 444 349 531 311 1050 147
Fragments arranged by size:
1050 531 444 349 311 147
SacI G_AGCT'C
Cuts at: 0 787 1861 2318 2832
Size: 787 1074 457 514
Fragments arranged by size:
1074 787 514 457
SacII CC_GC'GG
Cuts at: 0 174 2832
Size: 174 2658
SanDI GG'GwC_CC
Cuts at: 0 2634 2832
Size: 2634 198
SbfI CC_TGCA'GG
Cuts at: 0 482 1769 2832
Size: 482 1287 1063
Fragments arranged by size:
1287 1063 482
SfcI C'TryA_G
Cuts at: 0 439 478 1636 1765 1807 2029 2832
Size: 439 39 1158 129 42 222 803
Fragments arranged by size:
1158 803 439 222 129 42 39
SfoI GGC'GCC
Cuts at: 0 168 1568 2832
Size: 168 1400 1264
Fragments arranged by size:
1400 1264 168
SmaI CCC'GGG
Cuts at: 0 364 1361 1714 2832
Size: 364 997 353 1118
Fragments arranged by size:
1118 997 364 353
SmlI C'TyrA_G
Cuts at: 0 17 577 1275 1954 2017 2832
Size: 17 560 698 679 63 815
Fragments arranged by size:
815 698 679 560 63 17
SphI G_CATG'C
Cuts at: 0 1149 1491 2832
Size: 1149 342 1341
Fragments arranged by size:
1341 1149 342
StuI AGG'CCT
Cuts at: 0 831 1007 2832
Size: 831 176 1825
Fragments arranged by size:
1825 831 176
StyI C'CwwG_G
Cuts at: 0 161 1413 1562 2832
Size: 161 1252 149 1270
Fragments arranged by size:
1270 1252 161 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 439 1049 1086 2832
Size: 439 610 37 1746
Fragments arranged by size:
1746 610 439 37
TatI w'GTAC_w
Cuts at: 0 2303 2645 2832
Size: 2303 342 187
Fragments arranged by size:
2303 342 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1014 1046 2832
Size: 1014 32 1786
Fragments arranged by size:
1786 1014 32
Tth111I GACn'n_nGTC
Cuts at: 0 847 1059 2832
Size: 847 212 1773
Fragments arranged by size:
1773 847 212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 997 1171 1524 2493 2832
Size: 997 174 353 969 339
Fragments arranged by size:
997 969 353 339 174
XmaI C'CCGG_G
Cuts at: 0 362 1359 1712 2832
Size: 362 997 353 1120
Fragments arranged by size:
1120 997 362 353
Enzymes that do cut:
AccI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI
ApoI AseI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FseI HaeII Hin4I HincII HindIII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PflMI PmlI PpuMI PshAI PsiI
PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AloI AscI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BmtI BsaBI BsiWI BsmBI BspEI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI DraI
DraIII DrdI EcoNI EcoRI EcoRV FspI FspAI HpaI
KpnI MluI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PspXI PsrI PvuI RsrII
SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI